Nocardioides panaciterrulae sp. nov., isolated from soil of a ginseng field, with ginsenoside converting activity

A Gram-positive, coccoid to rod-shaped, non-spore-forming bacterium, designated Gsoil 958^T, was isolated from soil of a ginseng field located in Pocheon province in South Korea. This bacterium was characterized in order to determine its taxonomic position by using a polyphasic approach. Strain Gsoil 958^T was observed to grow well at 25–30 °C and at pH 7.0 on R2A and nutrient agar without NaCl supplementation. Strain Gsoil 958^T was determined to have β-glucosidase activity and the ability to transform ginsenoside Rb₁ (one of the dominant active components of ginseng) to F₂ via gypenoside XVII and Rd. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 958^T was shown to belong to the family Nocardioidaceae and related most closely to Nocardioides koreensis MSL-09^T (97.6 % 16S rRNA gene sequence similarity), Nocardioides aquiterrae GW-9^T (97.0 %), and Nocardioides sediminis MSL-01^T (97.0 %). The sequence similarities with other validly named species within the genus Nocardioides were less than 96.8 %. Strain Gsoil 958^T was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H₄) as the predominant menaquinone, and iso-C_16:0, iso-C_16:1 H, iso-C_14:0, iso-C_15:0 were identified as the major fatty acids. The G + C content of genomic DNA was determined to be 70.8 mol %. The chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain Gsoil 958^T to the genus Nocardioides. The results of both physiological and biochemical tests allowed for differentiation of strain Gsoil 958^T from the recognized Nocardioides species. Therefore, strain Gsoil 958^T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides panaciterrulae sp. nov. is proposed, with the type strain Gsoil 958^T (KACC 14271^T = KCTC 19471^T = DSM 21350^T).     

Lacinutrix jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment and emended description of the genus Lacinutrix

A Gram-negative, strictly aerobic, non-motile, rod-shaped and psychrophilic bacterial strain, PAMC 27137^T, was isolated from the marine sediment of the Ross Sea, Antarctica. Strain PAMC 27137^T was observed to grow at 4–10 °C, at pH 6.5–7.5 and in the presence of 2.5–4.0 % (w/v) sea salts. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PAMC 27137^T belongs to the genus Lacinutrix showing the high similarities with Lacinutrix mariniflava JCM 13824^T (97.6 %) and Lacinutrix algicola JCM 13825^T (97.1 %). Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27137^T is clearly distinguished from the most closely related Lacinutrix species. The major fatty acids (>5 %) were identified as iso-C_15:1 G (19.9 %), iso-C_15:0 (19.3 %), iso-C_17:0 3-OH (11.3 %), summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1 ω9c as defined by MIDI, 9.1 %), iso-C_15:0 3-OH (7.5 %), and anteiso-C_15:1 A (5.8 %). The polar lipids were found to consist of phosphatidylethanolamine, an unidentified aminolipid, an unidentified aminophospholipid, and five unidentified phospholipids. The major respiratory quinone was identified as MK-6. The genomic DNA G+C content was determined to be 32.1 mol%. Based on the data from this polyphasic taxonomic study, strain PAMC 27137^T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix jangbogonensis sp. nov. is proposed. The type strain is PAMC 27137^T (=KCTC 32573^T=JCM 19883^T).     

Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India

While studying the microbial diversity of hot springs of North-east India we isolated a strain AK31^T from the Jakrem hot spring of Meghalaya. The strain formed light yellow colonies on nutrient agar and was Gram negative, non spore-forming rods, motile with single polar flagellum. The strain was positive for oxidase and catalase and hydrolysed starch and weakly urea. The predominant cellular fatty acids were C16:0 (34.8 %), C17:0 cyclo (27.1 %), C16:1 ω7c and/or iso-C15:0 2OH (summed feature 3) (9.6 %), C10:0 3OH (8.0 %), C12:0 (5.8 %), C14:0 (5.3 %) and C18:1 ω7c (5.3 %). Strain AK31^T contained ubiquinone-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and one unidentified glycolipid as the polar lipids. The G + C content of the DNA of the strain AK31^T was 66.7 mol%. The 16S rRNA gene sequence analysis indicated that strain AK31^T was member of the genus Caldimonas and closely related to Caldimonas manganoxidans JCM 10698^T and Caldimonas taiwanensis On1^T with 96.9 % similarity and with Aquincola tertiaricarbonis L10^T and Azohydromonas australica IAM 12664^T with 96.5 and 96.4 % similarity respectively. Phylogenetic analyses indicated that the strain AK31^T clustered with C. manganoxidans JCM 10698^T and C. taiwanensis On1^T with a phylogenetic distance of 3.25 %. Based on data from the current polyphasic study, strain AK31^T is proposed as a novel species of the genus Caldimonas, for which the name Caldimonas meghalayensis sp. nov. is proposed. The type strain of C. meghalayensis is AK31^T (= MTCC 11703^T = JCM 18786^T).     

<Emphasis Type="Italic">Microbacterium rhizosphaerae</Emphasis> sp. nov., isolated from a Ginseng field,South Korea

A novel Gram-stain positive, aerobic, short rod-shaped, non-motile bacterium, designated strain CHO1^T, was isolated from rhizosphere soil from a ginseng agriculture field. Strain CHO1^T was observed to form yellow colonies on R2A agar medium. The cell wall peptidoglycan was found to contain alanine, glycine, glutamic acid, d-ornithine and serine. The cell wall sugars were identified as galactose, mannose, rhamnose and ribose. Strain CHO1^T was found to contain MK-11, MK-12, MK-13 as the predominant menaquinones and anteiso-C_15:0, iso-C_16:0, and anteiso-C_17:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, an unidentified phospholipid and three unidentified glycolipids were found to be present in strain CHO1^T. Based on 16S rRNA gene sequence analysis, strain CHO1^T was found to be closely related to Microbacterium mangrovi DSM 28240^T (97.81 % similarity), Microbacterium immunditiarum JCM 14034^T (97.45 %), Microbacterium oryzae JCM 16837^T (97.33 %) and Microbacterium ulmi KCTC 19363^T (97.10 %) and to other species of the genus Microbacterium. The DNA G+C content of CHO1^T was determined to be 70.1 mol %. The DNA–DNA hybridization values of CHO1^T with M. mangrovi DSM 28240^T, M. immunditiarum JCM 14034^T, M. oryzae JCM 16837^T and M. ulmi KCTC 19363^T were 46.7 ± 2, 32.4 ± 2, 32.0 ± 2 and 29.2 ± 2 %, respectively. On the basis of genotypic, phenotypic and phylogenetic properties, it is concluded that strain CHO1^T represents a novel species within the genus Microbacterium, for which the name Microbacterium rhizosphaerae sp. nov. is proposed. The type strain of M. rhizosphaerae is CHO1^T (= KEMB 7306-513^T = JCM 31396^T).     

Microlunatus cavernae sp. nov., a novel actinobacterium isolated from Alu ancient cave, Yunnan, south-west China

A Gram-positive, coccoid, non-endospore-forming actinobacterium, designated YIM C01117^T, was isolated from a soil sample collected from Alu ancient cave, Yunnan province, south-west China. Based on the 16S rRNA gene sequence analysis, strain YIM C01117^T was shown to belong to the genus Microlunatus, with highest sequence similarity of 97.4 % to Microlunatus soli DSM 21800^T. The whole genomic DNA relatedness as shown by the DNA–DNA hybridization study between YIM C01117^T and M. soli DSM 21800^T had a low value (47 ± 2 %). Strain YIM C01117^T was determined to contain LL-diaminopimelic acid with Gly, Glu and Ala amino acids (A3γ′ type) in the cell wall. Whole-cell hydrolysates were found to contain glucose, galactose, mannose and ribose. The major polar lipids were determined to be phosphatidylglycerol and diphosphatidylglycerol. The predominant menaquinone system present is MK-9(H₄), while the major fatty acids were identified to be anteiso-C_15:0 (24.1 %), iso-C_16:0 (22.3 %) and iso-C_15:0 (11.4 %). The G+C content of the genomic DNA was determined to be 65.9 mol%. The chemotaxonomic and genotypic data support the affiliation of the strain YIM C01117^T to the genus Microlunatus. The results of physiological and biochemical tests allow strain YIM C01117^T to be differentiated phenotypically from recognized Microlunatus species. Strain YIM C01117^T is therefore considered to represent a novel species of the genus Microlunatus, for which the name Microlunatus cavernae sp. nov. is proposed. The type strain is YIM C01117^T (= DSM 26248^T = JCM 18536^T).     

Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria

A novel halophilic actinomycete strain, H23^T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23^T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23^T had 16S rRNA gene sequence similarities ranging from 97.8 % (Actinopolyspora xinjiangensis TRM 40136^T) to 94.8 % (Actinopolyspora mortivallis DSM 44261^T). The strain grew optimally at pH 6.0–7.0, 28–32 °C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23^T had MK-10 (H₄) and MK-9 (H₄) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C_17:0 (37.4 %), iso-C_17:0 (14.8 %), iso-C_15:0 (14.2 %), and iso-C_16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23^T (=DSM 45501^T = CCUG 63368^T = MTCC 11562^T).     

Aliiglaciecola coringensis sp. nov., isolated from a water sample collected from mangrove forest in Coringa,Andhra Pradesh,India

A Gram-negative, rod shaped, motile, aerobic bacterium, designated as strain AK49^T was isolated from a water sample from a mangrove forest in Coringa village, Andhra Pradesh, India. Strain AK49^T was observed to form yellow coloured, smooth, circular, convex colonies on marine agar, with entire margins. Cells of strain AK49^T are 0.5–1.0 µm wide and 1.5–3.5 µm long. Growth was observed at 25–37 °C (optimum 30 °C), 2–6 % NaCl (optimum 2 %) and pH 6–8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain AK49^T is closely related to two species recently reclassified as members of the genus Aliiglaciecola: Aliiglaciecola lipolytica JCM 15139^T (sequence similarity 95.43 %) and Aliiglaciecola litoralis JCM 15896^T (sequence similarity 96.91 %). The major cellular fatty acids of strain AK49^T were found to include C_16:0, C_18:1ω7c and summed feature 3 (C_16:1ω7c/C_15:0 iso-2-OH). The polar lipid content of cell membrane was found to include phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified lipid and an unidentified glycolipid. The genomic DNA G+C content of strain AK49^T was determined to be 41.9 mol%. Based on the taxonomic methods, including chemotaxonomic, phenotypic and phylogenetic approaches, strain AK49^T is described here as a novel species belonging to the genus Aliiglaciecola, for which the name Aliiglaciecola coringensis sp. nov. is proposed. The type strain of Aliiglaciecola coringensis sp. nov. is AK49^T (=MTCC 12003^T = JCM19197^T).     

Echinicola shivajiensis sp. nov., a novel bacterium of the family “Cyclobacteriaceae” isolated from brackish water pond

Strain AK12^T, an orange pigmented Gram-negative, rod shaped, non-motile bacterium, was isolated from a mud sample collected from a brackish water pond at Rampur of West Bengal, India. The strain was positive for oxidase, catalase and phosphatase. The predominant fatty acids were iso-C_15:0 (42.7%), iso-C_17:0 3OH (13.2%), C_16:1ω7c/C_16:1ω6c (summed feature 3) (8.0%), iso-C_17:1 I/anteiso-C_17:1 B (summed feature 4) (6.1%) and iso-C_17:1ω9c/C_16:0 10-methyl (summed feature 9) (9.4%). Strain AK12^T contained MK-7 as the major respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids as the polar lipids. The G + C content of DNA of the strain AK12^T was 46.2 mol%. The 16S rRNA gene sequence analysis indicated that strain AK12^T was member of the genus Echinicola and closely related to Echinicola vietnamensis, Echinicola pacifica and Echinicola jeungdonensis with pair-wise sequence similarity of 96.8, 96.3 and 96.0% respectively. Phylogenetic analyses indicated that the strain AK12^T clustered with E. vietnamensis and together with E. pacifica and E. jeungdonensis with a phylogenetic distance of 5.1, 6.3 and 6.6% (94.9, 93.7 and 93.4% similarity) respectively. Based on data from the current polyphasic study, strain AK12^T is proposed as a novel species of the genus Echinicola, for which the name Echinicola shivajiensis sp. nov. is proposed. The type strain of E. shivajiensis is AK12^T (= MTCC 11083^T = JCM 17847^T).     

Geojedonia litorea gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coastal seawater

A Gram-negative, non-flagellated, non-gliding and rod-shaped, coccoid or filamentous bacterial strain, designated YCS-16^T, was isolated from coastal seawater from a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain YCS-16^T was observed to grow optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain YCS-16^T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Bizionia echini (96.1 %), Formosa spongicola (95.8 %), Bizionia algoritergicola (95.5 %) and Psychroserpens mesophilus (95.4 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain YCS-16^T joined the cluster comprising the type strains of Psychroserpens species. Strain YCS-16^T was found to contain MK-6 as the predominant menaquinone and iso-C_17:0 3-OH, iso-C_15:1 G and iso-C_15:0 as the major fatty acids. The major polar lipids detected in strain YCS-16^T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain YCS-16^T was determined to be 35.7 mol%. The phylogenetic analysis, chemotaxonomic data and other phenotypic properties revealed that strain YCS-16^T constitutes a new genus and species within the family Flavobacteriaceae, for which the name Geojedonia litorea gen. nov., sp. nov. is proposed. The type strain of Geojedonia litorea is YCS-16^T (=KCTC 32260^T = CCUG 63682^T).     

Sphingobacterium pakistanensis sp. nov., a novel plant growth promoting rhizobacteria isolated from rhizosphere of Vigna mungo

The taxonomic status of a bacterium, strain NCCP-246^T, isolated from rhizosphere of Vigna mungo, was determined using a polyphasic taxonomic approach. The strain NCCP-246^T can grow at 16–37 °C (optimum 32 °C), at pH ranges of 6–8 (optimum growth occurs at pH 7) and in 0–4 % (w/v) NaCl. Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP-246^T belonged to genus Sphingobacterium. Strain NCCP-246^T showed highest similarity to the type strain of Sphingobacterium canadense CR11^T (97.67 %) and less than 97 % with other species of the genus. The DNA–DNA relatedness value of strain NCCP-246^T with S. canadense CR11^T and Sphingobacterium thalpophilum JCM 21153^T was 55 and 44.4 %, respectively. The chemotaxonomic data revealed the major menaquinone as MK-7 and dominant cellular fatty acids were summed feature 3 [C_16:1 ω7c/C_16:1 ω6c] (37.07 %), iso-C_15:0 (28.03 %), C_16:0 (11.85 %), C_17:0 cyclo (8.84 %) and C_14:0 (2.42 %). The G+C content of the strain was 39.2 mol%. On the basis of DNA–DNA hybridization, phylogenetic analyses, physiological and, biochemical data, strain NCCP-246^T can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species, for which the name, Sphingobacterium pakistanensis sp. nov. is proposed with the type strain NCCP-246^T (= JCM18974 ^T = KCTC 23914^T).     

Ochrovirga pacifica gen. nov., sp. nov., A Novel Agar-Lytic Marine Bacterium of the Family Flavobacteriaceae Isolated From A Seaweed

A strain designated as S85^T was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, and non-motile and formed yellow colonies on the SWY agar (0.2 % yeast extract and 1.5 % agar in seawater) and Marine agar 2216. The strain grew at pH 5–9 (optimum, pH 8), at 15–40 °C (optimum, 25–28 °C), and with 1–9 % (w/v) NaCl (optimum, 3 %). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85^T was related to Lutibacter litoralis CL-TF09^T and Maritimimonas rapanae A31^T with 91.4 % and with 90.5 % similarity, respectively. The dominant fatty acids were iso-C_15:0, iso-C_15:0 3-OH and iso-C_17:0 3-OH, C_16:0 3-OH and summed feature 3 (C_16:1 ω7c and/or iso-C_15:0 2-OH). The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol %. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85^T stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov. The type strain of O. pacifica is S85^T (=KCCM 90106 =JCM 18327^T).     

Flavobacterium kyungheensis sp. nov., isolated from soil of a ginseng field

A Gram-staining negative, strictly aerobic, motile by gliding, non-spore-forming, pale yellow pigmented and rod-shaped bacterium designated strain THG-107^T was isolated from soil of a ginseng field on Ganghwa Island in the Republic of Korea and its taxonomic position was investigated by using a polyphasic study. Growth of strain THG-107^T was found to occur at 4–37 °C (optimum, 20–30 °C), at pH 5.5–10 (optimum, pH 7.0) and in the presence of 0–1 % (w/v) NaCl (optimum, absence) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain THG-107^T was shown to belong to the family Flavobacteriaceae and was related to Flavobacterium denitrificans ED5^T (99.1 % similarity). The G+C content of the genomic DNA was determined to be 34.2 mol%. These results are consistent with characteristics of members of the genus Flavobacterium. The only isoprenoid quinone detected in strain THG-107^T was menaquinone-6 (MK-6) and the major polyamine was identified as homospermidine (82.9 %). The major polar lipid detected was phosphatidylethanolamine and the major cellular fatty acids were identified as iso-C_15:0 (26.3 %), iso-C_17:0 3OH (12.6 %) and summed feature 3 (comprising C_16:1 ω7c and/or C_16:1 ω6c; 11.6 %). Flexirubin-type pigments were found to be present. Strain THG-107^T has β-glucosidase activity to convert ginsenosides Rb₁ and Rd into Gyp17 and F₂. DNA-DNA hybridization with F. denitrificans ED5^T was 52 %. Strain THG-107^T could be distinguished from F. denitrificans ED5^T and the other species of the genus Flavobacterium by its phylogenetic and genetic distinctiveness and by several phenotypic properties. Therefore, strain THG-107^T is considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium kyungheensis sp. nov. is proposed (type strain THG-107^T = KACC 16219^T = LMG 26575^T).     

Pontibacter humi sp. nov., Isolated from Mountain Soil

A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated as SWU8^T, was isolated from a mountain soil collected in Seoul Women’s University campus at South Korea. Phylogenic analysis, using 16S rRNA gene sequence of the new isolate, showed that strain SWU8^T belongs to the genus Pontibacter. The highest sequence similarities were 96.2 % with Pontibacter saemangeumensis GCM0142^T, 95.5 % with Pontibacter toksunensis ZLD-7^T, 95.3 % with Pontibacter roseus DSM 17521^T, and 95.1 % with Pontibacter odishensis JC130^T. Chemotaxonomic data showed that the most abundant fatty acids were summed feature 4 (comprising iso-C_17:1 I/anteiso-C_17:1 B; 26.9 %), iso-C_15:0 (25.6 %), and iso-C_17:0 3OH (10.6 %), and major polar lipid was phosphatidylethanolamine. The DNA G+C content of strain SWU8^T was 48.5 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain SWU8^T presents a novel species of the genus Pontibacter, for which the name Pontibacter humi sp. nov. is proposed. The type strain is SWU8^T (=KEMC 9004-131^T = JCM 19178^T).     

Tenacibaculum halocynthiae sp. nov., a member of the family Flavobacteriaceae isolated from sea squirt Halocynthia roretzi

A Gram-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated P-R2A1-2^T, was isolated from sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. It grew optimally at 25–28 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that the strain fell within the clade comprising Tenacibaculum species. Strain P-R2A1-2^T exhibited the highest 16S rRNA gene sequence similarity values of 97.6, 97.2 and 97.0 % to Tenacibaculum aestuarii SMK-4^T, T. lutimaris TF-26^T and T. aiptasiae a4^T, respectively, and of 94.5–96.8 % to the type strains of the other Tenacibaculum species. Strain P-R2A1-2^T contained MK-6 as the predominant menaquinone and C_16:1 ω7c and/or iso-C_15:0 2-OH, iso-C_15:0 3-OH and iso-C_15:0 as the major fatty acids. The DNA G + C content of strain P-R2A1-2^T was 30.7 mol % and its DNA–DNA relatedness values with the type strains of T. aestuarii, T. lutimaris and T. aiptasiae were 17 ± 4.2, 21 ± 6.1 and 16 ± 5.2 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separate from other Tenacibaculum species. On the basis of the data presented, strain P-R2A1-2^T is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum halocynthiae sp. nov. is proposed. The type strain is P-R2A1-2^T (=KCTC 32262^T = CCUG 63681^T).     

Flavobacterium qiangtangensis sp. nov., Isolated from Qiangtang Basin in Qinghai-Tibetan Plateau,China

A flexirubin-type yellow-pigmented, non-gliding, non-flagellated, gram-negative bacterium strain, designated F3^T, was isolated from a drilling core sample of the Qiangtang basin, Qinghai-Tibetan plateau, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain F3^T belongs to the genus Flavobacterium, with the highest 16S rRNA gene sequence similarity to the Flavobacterium noncentrifugens CGMCC 1.10076^T (94.92 %). Strain F3^T grew optimally at temperature about 20 °C, at pH about 7.0–8.0, at NaCl concentration 0 % (w/v). The DNA G+C content of the isolate was 35.5 mol%. The major polar lipid was phosphatidylethanolamine, predominant cellular fatty acids of the strain was iso-C_15:0 (22.02 %), while the major menaquinone was menaquinone 6. Due to the phenotypic and genetic distinctiveness and several other characteristic studied in this article, we consider F3^T as a novel species of the genus Flavobacterium, and propose to name it Flavobacterium qiangtangensis sp. nov. The type strain is F3^T (=CGMCC 1.12706^T = JCM 19739^T).     

Nocardioides soli sp. nov., a bacterium isolated from a mountain soil

Strain SR-1^T, a Gram-positive, strictly-aerobic, short-rod shaped, non-motile bacterium, was isolated from a mountain soil collected in Seoul Women’s University in South Korea. Growth occurred between 15 and 37 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and with 0–2 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SR-1^T belongs to the genus Nocardioides and is closely related to Nocardioides simplex KCTC 9106^T (96.8 %), Nocardioides caeni MN8^T (96.7 %), Nocardioides aromaticivorans H-1^T (96.6 %), and Nocardioides kongijuensis A2-4^T (96.6 %). Chemotaxonomic data revealed that strain SR-1^T possesses MK-8(H₄) as predominant menaquinone, ll-2,6-diaminopimelic acid as the diagnostic diamino acid, phosphatidylglycerol and diphosphatidylglycerol as predominant polar lipids and iso-C_16:0, 10-methyl-C_18:0, and C_18:1 ω9c are major fatty acids. The DNA G+C content of the strain SR-1^T was 72.4 mol%. Based on polyphasic evidence, strain SR-1^T (= KEMC 9004-134^T = JCM 19684^T) should be classified as the type strain of a novel Nocardioides species, for which the name Nocardioides soli sp. nov. is proposed.     

<Emphasis Type="Italic">Streptomyces ginkgonis</Emphasis> sp. nov., an endophyte from <Emphasis Type="Italic">Ginkgo biloba</Emphasis>

A novel endophytic actinomycete strain, designated KM-1-2^T, was isolated from seeds of Ginkgo biloba at Yangling, China. A polyphasic approach was used to study the taxonomy of strain KM-1-2^T and it was found to show a range of phylogenetic and chemotaxonomic properties consistent with those of members of the genus Streptomyces. The diamino acid of the cell wall peptidoglycan was identified as LL-diaminopimelic acid. No diagnostic sugars were detected in whole cell hydrolysates. The predominant menaquinones were identified as MK-9(H₆) and MK-9(H₈). The diagnostic phospholipids were found to be phosphatidylethanolamine and phosphatidylcholine. The DNA G + C content of the novel strain was determined to be 72.9 mol%. The predominant cellular fatty acids (> 10.0?%) were identified as iso-C_14?:?0, iso-C_16?:?0, C_16?:?0 and C_17?:?0 cyclo. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain is closely related to Streptomyces carpaticus JCM 6915^T (99.3%), Streptomyces harbinensis DSM 42076^T (98.9%) and Streptomyces cheonanensis JCM 14549^T (98.5%). DNA-DNA hybridizations with these three close relatives gave similarity values of 39.1 ± 1.9, 35.8 ± 2.3, and 47.4 ± 2.7%, respectively, which indicated that strain KM-1-2^T represents a novel species of the genus Streptomyces. This is consistent with the morphological, physiological and chemotaxonomic data. Cumulatively, these data suggest that strain KM-1-2^T represents a novel Streptomyces species, for which the name Streptomyces ginkgonis sp. nov. is proposed, with the type strain KM-1-2^T (= CCTCC AA2016004^T = KCTC 39801^T).     

Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater

A novel Gram-negative, rod shaped, motile, non-sporing strictly aerobic bacterium, designated strain AK5^T, was isolated from a sea water sample collected near Visakhapatnam coast, Bay of Bengal, India. Colonies on marine agar were circular, 3–4 mm in diameter, creamish and rose with entire margin. Growth occurred at 10–40°C, 0.5–12% (w/v) NaCl and pH of 7–11. Strain AK5^T was oxidase and catalase positive. The fatty acids were dominated by iso-branched saturated and unsaturated fatty acids with a high abundance of iso-C_15:0, iso-C_17:0 and summed feature 9 (as defined by MIDI). Q8 was found to be the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unidentified phospholipids as polar lipids. The DNA G+C content of strain AK5^T was 54.7 ± 0.2 mol%. Phylogenetic analysis revealed that strain AK5^T was a member of the genus Aliidiomarina and closely related to Aliidiomarina taiwanensis with a phylogenetic distance of 5.3% (94.7% 16S rRNA gene sequence similarity) and clustered with the same species. Results from the polyphasic taxonomy study support the conclusion that strain AK5^T represents a novel Aliidiomarina species, for which the name Aliidiomarina haloalkalitolerans sp. nov. is proposed. The type strain of A. haloalkalitolerans is AK5^T (= MTCC 11064^T = JCM 17359^T).     

Niabella thaonhiensis sp. nov., Isolated From the Forest Soil of Kyonggi University in Korea

Strain NHI-24^T was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24^T is closely related to members of the genus Niabella: N. drilacis E90^T (97.7 %), N. tibetensis 15-4^T (96.7 %), N. aurantiaca R2A15-11^T (96.5 %), N. hirudinis E96^T (95.8 %), N. soli JS13-8^T (94.7 %), N. ginsengisoli NBRC106414^T (94.4 %), and N. yanshanensis CCBAU 05354^T (94.2 %). Growth temperatures range widely, from 15 to 37 °C, with 30 °C as the optimum. Salt tolerance ranges from 0 to 2 %. The strain grows at pH 6.5–11.0, with an optimal range of pH 7.0–9.5. Cells produce flexirubin-type pigments, and the predominant menaquinone is MK-7. The major fatty acids of strain NHI-24^T are iso-C_15:0 (36.72 %), iso-C_15:1 G (20.8 %), and summed feature 3 (C_16:1 ω7c/C_16:1 ω6c; 15.2 %). DNA–DNA hybridization values between strain NHI-24^T and members of the genus Niabella range from 37 to 53 %. Based on these results, it is proposed that strain NHI-24^T represents a novel species of the genus Niabella with the name Niabella thaonhiensis (= KACC 17215^T = KEMB 9005-018^T = JCM 18864^T).     

Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium

Two Gram-positive, rod-shaped moderately halophilic bacterial strains, designated AD7-25^T and AB-11, were isolated from Aiding and Manasi salt lakes in Xinjiang of China, respectively. The strains were found to be able to grow at NaCl concentrations of 0–21 % (w/v), with optimum growth occurring at 6–8 % (w/v) NaCl. The optimal temperature and pH for growth were determined to be 33–37 °C and pH 7.0–7.5. Cells of the strains are motile by means of polar flagella. Both strains can produce ellipsoidal spores. The major cellular fatty acids were identified as anteiso-C_15:0, iso-C_15:0, iso-C_14:0, anteiso-C_17:0 and iso-C_16:0. The diamino acid in the peptidoglycan and the major quinone system were determined to be meso-diaminopimelic acid (meso-DAP) and MK-7, respectively. The DNA G+C contents of stains AD7-25^T and AB-11 were 39.8 and 40.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that these two novel strains are closely related to the genus Oceanobacillus showing 90–99.5 % similarity with respect to type strains. These two novel strains were most closely related to Oceanobacillus oncorhynchi subsp. incaldanensis DSM 16557^T (99.1 and 99.5 %), followed by O. oncorhynchi subsp. oncorhynchi JCM 12661^T (99.1 and 99.4 %), Oceanobacillus neutriphilus CGMCC 1.7693^T (97.0 and 97.5 %), Oceanobacillus sojae JCM 15792^T (97.6 and 98.0 %) and Oceanobacillus locisalsi KCTC 13253^T (96.5 and 96.9 %). The DNA–DNA hybridization data indicated that DNA relatedness between strains AD7-25^T and AB-11 was 91.0 %, and the genomic homology of representative strain AD7-25^T with O. oncorhynchi subsp. incaldanensis DSM 16557^T, O. oncorhynchi subsp. oncorhynchi JCM 12661^T, O. neutriphilus CGMCC 1.7693^T, O. sojae JCM 15792^T and O. locisalsi KCTC 13253^T were 41, 39, 20, 23 and 17 %, respectively. On the basis of phenotypic and phylogenetic distinctiveness, strains AD7-25^T and AB-11 should be assigned to the genus Oceanobacillus as a new species, for which the name Oceanobacillus aidingensis sp. nov. was proposed. The type strain is AD7-25^T (=CGMCC 1.9106 ^T = NBRC 105904^T).