CO2浓度倍增对城市银杏（Ginkgo biloba）叶片膜脂过氧化与抗氧化酶活性的影响

以生长在沈阳市区内的银杏为试材,使用开顶箱模拟法对倍增CO₂浓度（700μmolmol^-1）和正常空气CO₂浓度(≈350μmolmol^-1）条件下银杏生长参数,超氧阴离子自由基（O₂^-•）产生速率,丙二醛（MDA）含量,抗坏血酸（ASA）含量,超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）及谷胱甘肽还原酶（GR）活性动态变化进行分析,探讨高浓度CO₂对银杏膜脂过氧化与抗氧化酶活性的影响。结果表明,在短期(60d)内CO₂浓度倍增使银杏细胞内O₂^-•产生速率与H₂O₂含量减少,而ASA含量与SOD、APX、GR活性升高。与对照相比,大多数测定显示出显著差别。但较长期（70d以上）CO₂浓度倍增处理则使试验结果发生逆转,活性氧O₂^-•产生速率略有升高,SOD、APX、GR活性略有下降,ASA含量仍略高于对照（但与对照相比差异并不显著）,长期CO₂浓度倍增处理可能使试验结果发生逆转。     

浓度倍增对城市油松抗氧化酶活性的影响

利用OTC（开顶箱）法模拟未来CO₂浓度,于CO₂倍增浓度（700 μmol·mol^-1）和正常空气CO₂浓度(≈350 μmol·mol^-1）条件下,测定了沈阳市区油松（Pinus tabulaeformis）针叶超氧自由基（O^·₂）产生速率、过氧化氢（H₂O₂）含量、超氧化物歧化酶（SOD）与抗坏血酸-谷胱甘肽循环（ASA-GSH cycle）主要酶活性动态变化,探讨高浓度CO₂对油松抗氧化酶活性的影响。结果表明：在短期(60 d)内CO₂浓度倍增使油松超氧自由基（O₂^·）产生速率与过氧化氢（H₂O₂）含量减少,而SOD、抗坏血酸过氧化物酶（APX）、单脱氢抗坏血酸还原酶（MDAR）、脱氢抗坏血酸还原酶（DHAR）、谷胱甘肽还原酶（GR）活性升高;植株抗氧化能力增强,对活性氧清除能力提高;但长期（70 d以上）CO₂浓度倍增处理则可能使试验结果发生逆转。     

新型真菌源激活蛋白诱导水稻抗病性及其生理机制

为明确新型真菌源激活蛋白对水稻抗病性的诱导作用及其生理机制,研究了激活蛋白对水稻稻瘟病和白叶枯病的诱导抗病性,监测了激活蛋白处理后水稻过氧化物酶（POD）、多酚氧化酶（PPO）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性及过氧化氢（H₂O₂）含量变化。结果表明,1～6 μg·mL^-1激活蛋白对稻瘟病和白叶枯病的诱抗效果分别为45.2%～71.4%和47.6%～66.3%,以6 μg·mL^-1激活蛋白的诱抗效果最好。与对照相比,2 μg·mL^-1激活蛋白处理水稻后3～15 d内不同程度诱导了防御酶POD、PPO和SOD活性,抑制CAT活性,提高H₂O₂含量。新型真菌源激活蛋白能够诱导水稻产生对稻瘟病和白叶枯病的抗病性,其诱导抗性机制与水稻体内的活性氧代谢密切相关。     

臭氧浓度升高对油松抗氧化系统活性的影响

以生长在开顶箱内的油松为试材,对高浓度臭氧(80 nmol·mol^-1)条件下油松(Pinus tabulaeformis)针叶中超氧阴离子自由基(O₂^·)产生速率、过氧化氢(H₂O₂)含量、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶、谷胱甘肽还原酶活性与抗坏血酸(ASA)含量进行测定.结果表明:高浓度臭氧使O₂^·产生速率提高,H₂O₂ 和MDA含量增加.ASA含量与SOD、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶、谷胱甘肽还原酶活性在高浓度臭氧熏蒸的前期升高,随后下降并低于对照.说明生长季前期,油松抗氧化系统对高浓度臭氧存在适应性反应,但不能抵抗长期臭氧胁迫带来的氧化伤害.     

近地层高臭氧浓度对水稻生长发育影响研究进展

臭氧（O₃）被认为是最主要的空气污染物之一.目前地球对流层大气中平均O₃浓度已经从工业革命前的38 nl·L^-1（25～45 nl·L^-1,夏季每天8 h平均）上升到2000年的50 nl·L^-1,悲观估计到2100年近地层O₃浓度将上升到80 nl·L^-1.水稻是世界上最重要的粮食作物,准确评估近地层O₃浓度升高对水稻生长发育的影响具有重要意义.本文从叶片伤害特征、光合作用、水分关系、生育期、物质生产与分配、叶片膜保护系统、籽粒产量及产量构成因素等方面,系统收集和整理了气室条件下（包括封闭气室、开放式气室）高O₃浓度对水稻生长发育影响的研究进展,并对该领域有待深入研究的方向进行了展望.     

Na2CO3胁迫下星星草幼苗活性氧及保护酶活性的变化

对低浓度Na₂CO₃胁迫下星星草幼苗相对电导率、O^-₂产生速率、H₂O₂含量以及保护酶CAT、SOD和POD活性的研究结果表明,低盐胁迫1 d后,星星草幼苗细胞膜的通透性、O^-₂产生速率、H₂O₂含量及保护酶活性都随着盐胁迫的加剧而升高,其具体的变化规律与盐胁迫强度和幼苗细胞膜的受损伤程度密切相关,但相关关系的性质上具有差异。     

外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响

采用营养液水培的方法,研究了外源一氧化氮（Nitric oxide, NO）对50mmol•L^-1NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响。结果表明：10~400μmol•L^-1 NO供体硝普钠（Sodium nitroprusside, SNP）能显著缓解NaCl胁迫对黄瓜植株造成的伤害,100μmol•L^-1 SNP缓解效果最好,可提高幼苗的生长量,增强幼苗叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）活性,提高了叶片叶绿素和脯氨酸（Pro）含量、净光合速率（Pn）、蒸腾速率（Tr）及气孔导度（Gs）;降低了叶片丙二醛（MDA）和过氧化氢（H₂O₂）的含量、超氧阴离子（O^•-₂）的产生速率、质膜透性和胞间二氧化碳浓度（Ci）。     

外源H2O2处理对唐古特白刺愈伤组织脯氨酸代谢的影响

以唐古特白刺（Nitraria tangutorum Bobr.）愈伤组织为材料,研究外源H₂O₂（2和10 μmol·L^-1）处理下其脯氨酸含量及相关代谢酶活性的变化,试图从细胞水平揭示H₂O₂影响脯氨酸代谢的生理机制。结果显示,2和10 μmol·L^-1 H₂O₂处理24 h使唐古特白刺愈伤组织脯氨酸含量分别变为对照的112%和92%,而处理72 h后,脯氨酸含量增加为对照的141%和119%;与对照相比,外源H₂O₂处理诱导愈伤组织脯氨酸脱氢酶活性降低,而谷氨酸激酶活性升高,但鸟氨酸转氨酶活性无显著变化;此外,H₂O₂处理使唐古特白刺愈伤组织内源性H₂O₂含量升高。结果表明,外源H₂O₂诱导了唐古特白刺愈伤组织H₂O₂含量的增高和脯氨酸的积累,且H₂O₂处理下脯氨酸脱氢酶活性的降低及谷氨酸激酶的升高与愈伤组织脯氨酸的积累有关。     

硝普钠缓解镧引起的黑麦草幼苗生长抑制和根系氧化损伤

采用水培方法,研究了外源一氧化氮（NO）供体硝普钠（SNP）对300 μmol·L^-1 LaCl₃胁迫下黑麦草幼苗生长和根系活性氧代谢的影响。结果表明：在LaCl₃胁迫下,喷施50 μmol·L^-1 SNP能够抑制镧（La）从黑麦草根系向地上部的转运,缓解La对幼苗生长的抑制作用;提高幼苗根系超氧化物歧化酶和抗坏血酸过氧化物酶活性,降低过氧化物酶活性及谷胱甘肽、脯氨酸、H₂O₂、丙二醛含量、超氧阴离子产生速率和质膜相对透性,对过氧化氢酶活性和抗坏血酸含量无显著影响。表明NO可通过活性氧代谢的调节,缓解高浓度La胁迫对黑麦草幼苗生长的抑制作用。     

EFFECTS OF CALCIUM NITRATE STRESS ON ASCORBATE-GLUTATHIONE CYCLE METABOLISM IN LEAVES OF HYDROPONICALLY-GROWN GRAFTED EGGPLANT SEEDLINGS

 以日本引进的设施专用耐盐茄(Solanum melongena)品种‘Torvum Vigor’为砧木, 栽培茄(S. torvum)品种‘苏崎茄’为接穗, 用营养液栽培, 对80 mmol&;#8226;L^–1 Ca(NO₃)₂胁迫下茄子嫁接苗和自根苗叶片抗坏血酸-谷胱甘肽循环系统中抗氧化酶活性和抗氧化物及H₂O₂含量进行比较。结果表明, Ca(NO₃)₂胁迫下茄子幼苗叶片H₂O₂含量有所增加, 但嫁接苗叶片H₂O₂含量显著低于自根苗。Ca(NO₃)₂胁迫下嫁接苗叶片抗氧化酶(APX、DHAR和GR)活性、AsA和GSH再生率、氧化还原力(AsA/DHA值和GSH/GSSG值)均显著高于自根苗。综上所述, Ca(NO₃)₂胁迫下嫁接苗保持良好的AsA-GSH循环效率, 清除H₂O₂效率较高, 细胞受氧化损伤程度较轻, 表现出较强的耐盐性。     

Influences of addition of different krilium in saline-sodic soil on the seed germination and growth of cabbage

China is one of the largest countries with huge amount of saline-sodic soil. How to ameliorate these lands is a hotspot in China. Through measurement of soil salinity, pH value and seed germination and growth status of cabbage seedlings, 17 kinds of krilium were added to experimented soil to evaluate their effects on amelioration of heavy saline-sodic soil. Firstly, among these 17 kinds of krilium, 11 (HPMA, gypsum, HEDP, T225, PAPEMP, MA-AA, ATMP, AA/AMPS, PAA, charcoal and BHMTPMPA) were effective agents and could effectively induce seed germination without any germination phenomena in the control saline-sodic soil. HPMA was the best one in ameliorating saline-sodic soil, which has been manifested by various indices of seed germination. Secondly, addition of gypsum together with other organic agents could strongly increase the ameliorative effectiveness if compared with relatively weak agents, while decrease seed germination if compared with the stronger agent of the 2 mixed agents, showing that the synergetic effect between gypsum and other kinds of tested krilium was not evident. Thirdly, optimum dosages for separate addition of HPMA and gypsum were 42.8 L m^?3 and 25 kg m^?3, while mixed-addition of these 2 agents at the same dosages did not cause plus effect in soil improvement. Finally, field application of HPMA could enhance the growth of cabbage seedlings at the first month, but began to wilt thereafter. Thus, instead of one-time addition, several-time addition of HPMA at the suitable dosage in field practice could strengthen the effectiveness of soil amelioration.     

盐碱地土壤改良剂施用对种子萌发和生长的影响

我国是盐碱地大国,如何改良盐碱地一直是研究热点。选择17种盐碱地改良剂施加于重度盐碱土,以白菜种子萌发指标和改良土壤电导率及pH值为评价指标,筛选出适宜盐碱地改良的土壤改良剂及其比例配比,为盐碱地治理改良提供理论依据。结果表明,（1）与对照盐碱土相比,11种有效改良剂（聚马来酸酐、石膏、羟基乙叉二膦酸、丙烯酸-丙烯酸羟丙酯共聚物、多氨基多醚基甲叉膦酸、聚马来酸酐-丙烯酸共聚物、氨基三甲叉膦酸、丙烯酸-2-丙烯酰胺-2-甲基丙磺酸多元共聚、聚丙烯酸、木炭、双1,6-亚己基三胺五甲叉膦酸）不仅使白菜种子发芽,对降低pH有显著作用,对电导率降低影响不明显;其中聚马来酸酐（HPMA）的改良效果最佳,种子发芽各项指标均较高,具有较强的种子生活力,其发芽率、发芽指数、活力指数的活力指标分别达到72.5%,36.2,1.2;种子发芽率,发芽势和发芽指数与土壤pH值有密切关系（p〈0.05）,但与土壤电导率无关（p〉0.05）,从而说明不同土壤改良剂的效果主要显现在调节pH的功能上。（2）在施加钙源石膏辅助下,9种改良剂（聚马来酸酐,聚丙烯酸,木炭,木醋液,乙二胺四乙酸,732型阳离子交换吸附树脂（H＋型）,柠檬酸,柠檬酸钙,氨三乙酸）改良效果有明显提升,改良剂改良后的盐碱土在种子发芽率、发芽指数、活力指数等指标均有所上升,说明外施钙源有助于其它改良剂效果的发挥。（3）不同浓度梯度筛选中,42.8L/m^3聚马来酸酐、25kg/m^3石膏改良效果最佳,能够有效降低土壤电导率和pH值,改良后盐碱土的种子发芽率、发芽势、发芽指数、活力指数均最接近壤土种子发芽率、发芽指数等各项指标,但二者单独施用改良盐碱土其发芽率高于二者混合施用的发芽率,不存在协同效应,故二者不宜混合施用。同时,野外进行改良应该考虑多次少量施用或增加改良剂浓度的方法来增强改良效果。     

Addition of HPMA affects seed germination, plant growth and properties of heavy saline-alkali soil in northeastern China: comparison with other agents and determination of the mechanism

China has a large area of inland saline-alkali land, equivalent to 40% of the total cultivated land in the country. The principal features of these lands are high salt content, high pH, and poor soil structure with low water infiltration and poor drainage. These conditions effectively prevent the exploitation of such land for agriculture. In this study, we have compared 17 soil conditioning agents for their abilities to promote seed germination and growth under both laboratory and field conditions. One of these, Hydrolyzed Polymaleic Anhydride (HPMA), was identified as a highly effective agent for soil improvement. Laboratory germination experiments and laboratory and field cultivation of a variety of plants both showed that addition of HPMA could significantly increase the germination percentage and plant growth rate. Distinct from other Ca-carrier agents such as gypsum, HPMA increases the dissolution of CaCO₃, which is abundant in the calcareous saline-alkali soils. This allows Ca²⁺ in soil solution to displace the over-abundant Na⁺ in the soil colloids. This process greatly improves soil properties such as the bulk density, which decreased, and the capillary soil rise height of water and soil water infiltration rate, which increased. Direct SEM and AFM imagery showed flocculent soil precipitation (soil aggregates) after HPMA addition, and a looser structure of those aggregates. The addition of HPMA also reduced the soil pH and EC. These changes in soil chemical and physical properties are a likely explanation for the soil improvement effected by HPMA. The high content of insoluble CaCO₃ in saline-alkali land such as that in northeastern China (up to 13%) favors the further exploration of HPMA as an ameliorative agent.     

不同寄主植物对截形叶螨生长发育及繁殖的影响

在28℃下用叶盘法研究了５种寄主植物对截形叶螨生长发育及繁殖的影响。结果表明,在不同寄主植物上,截形叶螨各螨态历期、单雌产卵量、日均产卵量、产卵期、净增殖率、周限增长率、内禀增长率、世代平均周期和种群加倍时间等均有明显差异; 雌螨完成一代所需时间长短依次为9.3天（黄瓜）、9.3天（菜豆）、9.6天（大豆）、11.0天（茄子）和11.6天（玉米）,产卵期依次为9.9天（茄子）、11.2天（黄瓜）、12.9天（菜豆）、15.8天（玉米）和17.9天（大豆）;单雌产卵量和净增殖率在大豆上最高,分别为115.0和51.7,而在茄子上最低,分别为38.0和11.7。幼、若螨期存活率大小依次为93.6%（大豆）、91.7%（玉米）、89.9%（黄瓜）、84.3%（菜豆）和61.6%（茄子）,存活曲线均为Ⅰ型。大豆是其最嗜食寄主植物,其次是玉米,再次为黄瓜和菜豆,对茄子的嗜食性最差。     

Effect of alkaline and saline substrates on ABA contents,distribution and transport in plant roots

The distribution of the phytohormone abscisic acid (ABA) between plant and soil and within plants growing on an alkaline substrate has been studied in order to separate the true effect of high soil pH from any effects that might be a result of the high salinity normally observed in alkaline soils. Leaves of a range of plants grown in an alkaline and saline solid substrate (municipal solid waste incinerator bottom slag) exhibited higher ABA levels than leaves of control plants. In contrast, roots of most plants grown on alkaline and saline substrates, particularly those without an exodermis (various species of Fabaceae), had slightly lower than or comparable ABA contents to control roots. However, in corn roots (Zea maysL. cv. Garant FAO 240) which possess a well-developed exodermis, alkaline and saline conditions in the rhizosphere did not reduce the endogenous ABA concentration, because the leaching of ABA from corn roots into the rhizosphere was lower than that from Vicia faba (variety Dreifache Weisse) roots. ABA efflux from corn and Vicia roots into the soil solution was observed only during the first days of the experiments and thereafter became substantially decreased. Because the leaching of ABA from Vicia faba roots into the rhizosphere was higher than that from corn roots, the leaves of Vicia plants grown in alkaline soil at low salinity no longer exhibited an elevated ABA concentration. However, whilst the roots of corn plants grown on desalted slag retained ABA levels that were higher than those of the control, the ABA content of leaves was not significant higher than the controls. For this reason, root ABA retention must be enough to induce tolerance to alkalinity in corn plants and there is no need to implicate changes in ABA concentrations in the aerial parts of the plant as having a role in this tolerance. In alkaline soil substrates, considerable portions of the ABA synthesised in the roots leached out into the soil solution of the rhizosphere according to the anion trap concept. An exodermis substantially reduces this leakage. The transient nature of ABA efflux into the rhizosphere was a result of the fact that the salt stress itself was only a transient phenomenon due to a washout of salt by irrigation. The results match predictions of mathematical models describing the effect of alkaline pH on the distribution of abscisic acid within plants and between roots and the rhizosphere. Species that can retain root ABA in the face of its tendency to leach into the more alkaline compartment are able to tolerate these normally harmful sites. This revised version was published online in June 2006 with corrections to the Cover Date.     

NaCl-and Gibberellic Acid-induced Changes in the Content of Auxin and the Activities of Cellulase and Pectin Lyase During Leaf Growth in Rice (Oryza sativa)

The interactive effect of NaCl salinity and gibberellic acidin the activities of cellulase and pectin lyase, and on thecontent of auxin and chlorophyll, has been determined duringleaf growth (fifth from base) in rice. The linear growth, chlorophyllcontent, activity of cellulase, and the auxin level of leaveswere markedly decreased when plants were exposed to salt stress(12 dS m^–1). However pectin lyase activity did not registerany significant alteration in the leaves of salt-stressed plantscompared with the control. Treatment of plants with gibberellicacid (GA₃) (10 ppm) increased the leaf growth and chlorophyllcontent with a concomitant rise in the activity of cellulaseunder stressed as well as non-stressed conditions. A markedincrease in the content of auxin was discernible in the leavesof salt-stressed plants treated with GA₃ compared with non-treatedsalinized ones. An appreciable increment in the activity ofpectin lyase in response to GA₃ administration was detectedonly in the leaves of non-stressed plants. These results indicatethat enhancement of cellulase activity and the augmentationof endogenous auxin content may be involved in the stimulationof rice leaf growth by GA₃ under saline conditions. Oryza sativa, rice, leaf growth, NaCl salinity, gibberellic acid, cellulase, pectin lyase, auxin     

盐土和沙土对新疆常见一年生盐生植物生长和体内矿质组成的影响

选用古尔班通古特沙漠南缘荒漠-绿洲交错带常见的一年生盐生植物盐角草、刺毛碱蓬、叉毛蓬、猪毛菜和碱地肤为材料,比较了它们在原状盐土和沙土中的生长及体内矿质元素组成的差异。结果表明：① 原状盐土0—100 cm各土层的pH值低于沙土,但电导率和含水量明显高于沙土;② 原状盐土中生长的植物干重是沙土中生长植物干重的7—118倍,后者的根冠比是前者的2—6倍。③ 体现肉质化程度的地上部含水率为52%—81%,中低耐盐植物含水率在两种土壤中差异显著,强耐盐植物差异不显著;④ 5种一年生盐生植物地上部氮浓度为11—34 g/kg,在有效氮含量高的盐土上植物氮浓度也高;磷浓度为1—4 g/kg,在有效磷高的盐土上植物磷浓度也较高（盐角草除外）;但沙土中的植物地上部钾浓度明显高于盐土中植株的地上部钾浓度,这与两种土壤在0—60 cm土层中的钾浓度差异相反;⑤ 尽管原状盐土0—100 cm土层中的水溶性钙、镁、钠、氯、硫浓度显著高于沙土,盐土与沙土中生长的植物地上部钠、水溶性氯和硫的浓度比值远远低于土壤中的相应元素浓度的比值,甚至盐土中的植株钙、镁浓度等于或显著低于沙土中生长的植物。表明盐土不仅影响一年生盐生植物的生长,也显著影响这些植物对矿质元素的吸收和累积。一年生盐生植物能够选择性吸收不同生境中的矿质元素。本研究期望为进一步深入研究盐生植物耐盐的适应机制提供依据,也可为植物修复盐碱土的品种选择提供参考。     

Effects of different treatments of fly ash and mining soil on growth and antioxidant protection of Indian wild rice

The aim of the present study was investigation of the effects of fly ash and mining soil on growth and antioxidant protection of two cultivars of Indian wild rice (Oryza nivara and Oryza rufipogon) for possible phytoremediation and restoration of metal-contaminated site. In this study, Indian wild rice showed significant changes in germination, growth, and biochemical parameters after exposure to different ratio of fly ash and mining soil with garden soil. There was significant reduction of germination, fresh weight, dry weight, leaf chlorophyll content, leaf area, Special Analysis Device Chlorophyll (SPAD) Index, proteins, and activities of antioxidant enzymes in both cultivars of the wild rice grown in 100% fly ash and mining soil compared to the plants grown in 100% garden soil. Results from this study showed that in both cultivars of wild rice, all growth and antioxidant parameters increased when grown in 50% fly ash and mining soil. Taken together, Indian wild rice has the capacity to tolerate 50% of fly ash and mining soil, and can be considered as a good candidate for possible phytoremediation of contaminated soils.     

Effects of Chemicals Often Regarded as Germination Stimulants on Seed Conditioning and Germination of Witchweed (Striga asiatica)

Gibberellin A₃ (GA₃), kinetin and zeatin promoted germinationof conditioned witchweed (Striga asiatica [L.] Kuntze) seedssimilar to dl-strigol and a natural stimulant from corn rootexudates. GA₃ promoted germination only with scarified seeds.However, these regulators, as well as dl-strigol and naturalstimulants, inhibited the conditioning of seeds and their subsequentgermination. On the other hand, abscisic acid induced 74% germinationof conditioned seeds but had no effect on seed conditioning.Other chemicals such as sucrose, NaNO₃, vitamin B₁, L-methionine,nicotinamide, indoleacetic acid and colchicine had no apparenteffect on seed conditioning, while sucrose, L-methionine andinositol induced about 30 % germination of conditioned seeds.Seeds generally required either a longer conditioning time ora higher terminal dl-strigol concentration to eliminate anyinhibition of seed conditioning induced by a compound. dl-Strigol, germination stimulation, parasitic plants, seed conditioning, seed germination, Striga asiatica (L.) Kuntze, weed control, growth regulating substances     

山东地区盐碱土花生种子际土壤微生物群落结构的研究

【目的】以不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤为实验对象,探讨花生种子在吸水膨胀与萌发过程中,不同类型盐碱土对种子际土壤微生物多样性变化的影响。【方法】采集不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤,通过对各样品中细菌的16S r RNA基因的V3-V4区进行PCR扩增,利用Illumina Hiseq高通量测序技术对12份V3-V4高变区PCR产物进行测序,并对测序数据进行生物信息学分析。【结果】(1)盐碱土壤的种子际细菌群落多样性高于非盐碱土壤,且以东营青坨滨海盐土种子际土壤细菌群落多样性较高。(2)不同类型土壤样本微生物群落结构在纲水平存在明显差异。4种土壤类型种子际土壤细菌共分属于6个菌纲,分别为Proteobacteria、Actinobacteria、Actinobacteria、Bacteroidetes、Acidobacteria和Firmicutes菌纲,并均以Proteobacteria和Actinobacteria菌纲为主要菌纲。全样本菌落结构分析结果表明,4种类型土壤中不同吸胀时间内种子际微生物菌落在门、属水平上的类型和丰度差异最为显著(P0.05)。(3)beta多样性分析和各样本遗传距离(phylogenetic distances)聚类树图分析表明,4个土壤类型的12个土壤样本种子际土壤中微生物群落均可聚为2大类。【结论】土壤含盐量越高其种子际土壤细菌群落多样性较高。不同类型土壤样本微生物群落结构在纲水平存在明显差异,以Proteobacteria和Actinobacteria菌纲为主要菌纲。种子吸胀萌发时间影响种子际微生物菌落在门、属水平上的类型和丰度,但对相同土壤类型样本间遗传距离无影响。