共查询到20条相似文献,搜索用时 312 毫秒
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Gongti Lai Shiren Song Yunxiao Liu Peining Fu Jiang Xiang Jiang Lu 《Journal of Phytopathology》2019,167(2):65-74
Based on previous cloning of VpRPW8‐e, we obtained a 1,126 bp VpRPW8‐e promoter sequence in this study. A large number of TATA‐boxes, CAAT‐boxes, and other cis‐acting elements were predicted including light‐responsive elements, hormone‐responsive elements, stress‐responsive elements, and growth‐ and development‐associated elements within the promoter sequence. To further investigate the function of this promoter, we examined its activity in response to biotic and abiotic stress. The VpRPW8‐e promoter was strongly activated by Plasmopara viticola infection, and activation also occurred when the orientation of the promoter was reversed, although to a lesser extent. Deletion analysis showed that the ?1,126 to ?475 bp region of VpRPW8‐e promoter had high activity. A promoter fragment 5′ deleted to ?475 bp (P?475) was activated in response to heat and cold stress, and even more strongly in response to Phytophthora capsici and salicylic acid (SA). Furthermore, Transgenic Nicotiana benthamiana were generated, VpRPW8‐e driven by P?475 enhanced resistance to Ph. capsici in N. benthamiana. Based on these results, the ?475 bp region was deduced to be an indispensable part of the VpRPW8‐e promoter. VpRPW8‐e promoter is involved in pathogen‐ and stress‐inducible expression. 相似文献
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ETHYLENE RESPONSE FACTOR 96 positively regulates Arabidopsis resistance to necrotrophic pathogens by direct binding to GCC elements of jasmonate – and ethylene‐responsive defence genes 下载免费PDF全文
Jérémy Catinot Jing‐Bo Huang Pin‐Yao Huang Min‐Yuan Tseng Ying‐Lan Chen Shin‐Yuan Gu Wan‐Sheng Lo Long‐Chi Wang Yet‐Ran Chen Laurent Zimmerli 《Plant, cell & environment》2015,38(12):2721-2734
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A library of synthetic transcription activator‐like effector‐activated promoters for coordinated orthogonal gene expression in plants 下载免费PDF全文
Kathleen Brückner Petra Schäfer Ernst Weber Ramona Grützner Sylvestre Marillonnet Alain Tissier 《The Plant journal : for cell and molecular biology》2015,82(4):707-716
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Y. Zhu Y. Li S. Zhang X. Zhang J. Yao Q. Luo F. Sun X. Wang 《Plant biology (Stuttgart, Germany)》2019,21(4):571-584
- The prevention of Botrytis cinerea infection and the study of grape seedlessness are very important for grape industries. Finding correlated regulatory genes is an important approach towards understanding their molecular mechanisms.
- Ethylene responsive factor (ERF) gene family play critical roles in defence networks and the growth of plants. To date, no large‐scale study of the ERF proteins associated with pathogen defence and ovule development has been performed in grape (Vitis vinifera L.). In the present study, we identified 113 ERF genes (VvERF) and named them based on their chromosome locations. The ERF genes could be divided into 11 groups based on a multiple sequence alignment and a phylogenetic comparison with homologues from Arabidopsis thaliana. Synteny analysis and Ka/Ks ratio calculation suggested that segmental and tandem duplications contributed to the expansion of the ERF gene family. The evolutionary relationships between the VvERF genes were investigated by exon–intron structure characterisation, and an analysis of the cis‐acting regulatory elements in their promoters suggested potential regulation after stress or hormone treatments.
- Expression profiling after infection with the fungus, B. cinerea, indicated that ERF genes function in responses to pathogen attack. In addition, the expression levels of most ERF genes were much higher during ovule development in seedless grapes, suggesting a role in ovule abortion related to seedlessness.
- Taken together, these results indicate that VvERF proteins are involved in responses to Botrytis cinerea infection and in grape ovule development. This information may help guide strategies to improve grape production.
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F. Shokouhifar M. R. Zamani M. Motallebi A. Mousavi M. A. Malboobi 《Biologia Plantarum》2011,55(4):689-695
In this study, we selected two known pathogen-inducible cis-acting elements, F and E17, to construct synthetic pathogen-inducible promoters for analysis in transformed canola (Brassica napus L.). The synthetic promoter approach was used, which involved the insertion of dimers and combining two cis-acting elements (E17 and F) upstream of the minimal CaMV 35S promoter. Canola plants were transformed by three constructs,
pGEE, pGFF, pGFFEE containing synthetic promoters (SP), SP-EE, SP-FF and SP-FFEE, respectively. Analyses of histochemical
and fluorometric GUS expression indicated that synthetic promoters responded to fungal elicitors and phytohormone treatments.
The SP-FF promoter showed high responses against methyl jasmonate and Sclerotinia sclerotiorum, while SP-EE demonstrated inducibility only in response to salicylic acid and Rhizoctonia solani. The SP-EE promoter similar to SP-FFEE, did not respond to S. sclerotiorum and methyl jasmonate. However, SP-FFEE was highly induced by R. solani elicitors and showed that the level of GUS expression was greater than that by either of E17 or F elements alone. These three
synthetic promoters did not activate the expression of the reporter gene in response to cold, heat, UV and wounding. 相似文献
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c‐fos gene has a close relationship with the osteoblasts. Mechanical signal effect on osteoblasts would change the expression level of c‐fos. Authors introduce the signal pathways of four cis‐response elements on the promoter of c‐fos, that is, CRE (cAMP responsive element), FAP‐1 (Fbs‐AP‐1 site), SRE (serum response element), and SIE (sis‐inducible element), as the regulatory mechanism for c‐fos gene expression following various stimuli. J. Cell. Biochem. 106: 764–768, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
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