用ELISA法测定抗人心肌肌球蛋白轻链(CMLC)单克隆抗体的K_D值

人心肌肌球蛋白由轻链（ｃａｒｄｉａｃｍｙｏｓｉｎｌｉｇｈｔｃｈａｉｎｓ,ＣＭＬＣ）和重链（ｃａｒｄｉａｃｍｙｏｓｉｎｈｅａｖｙｃｈａｉｎｓ,ＣＭＨＣ）组成．当心肌细胞遭到破坏时,ＣＭＬＣ释放入血,可致血中ＣＭＬＣ浓度增高．血中ＣＭＬＣ的测定是一种高度...     

集约高产过程中土壤有机质动态初探

集约高产过程中土壤有机质动态初探崔玉亭,韩纯儒（北京农业大学,１０００９４）ＤｙｎａｍｉｃｓｏｆＳｏｉｌＯｒｇａｎｉｃＭａｔｔｅｒ（ＳＯＭ）ｉｎｔｈｅＰｒｏｃｅｓｓｏｆＡｇｒｉｃｕｌｔｕｒａｌＩｎｔｅｎｓｉｆｉｃａｔｉｏｎ￥．ＣｕｉＹｕｔｉｎｇ;ＨａｎＣｈｕｎｒｕ（ＢｅｉｊｉｎｇＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ,１０００９４）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（２）：３７—３８．Ｉｎｔｈｉｓｐａｐｅｒ,ｔｈｅｂａｌａｎｃｅｏｆｓｏｉｌｏｒｇａｎｉｃｍａｔｔｅｒ（ＳＯＭ）ｉｎｔｈｅｐｒｏｃｅｓｓｏｆａｇｒｉｃｕｌｔｕｒａｌｉｎｔｅｎｓｉｆｉｃａｔｉｏｎｉｎＣｈａｎｇｚｈｏｕｒｅｇｉｏｎｉｓｓｔｕｄｉｅｄａｎｄａｍｅｄｉｕｍ－ａｎｄｌｏｎｇ－ｔｅｒｍｐｒｅｄｉｃｔｉｏｎｏｆｉｔｓｄｙｎａｍｉｃｓｉｓｍａｄｅ。Ｔｈｅｒｅ－ｓｕｌｔｓｓｈｏｗｔｈａｔｄｕｒｉｎｇｔｈｉｓｐｒｏｃｅｓｓ,ｔｈｅｂａｌａｎｃｅｏｆＳＯＭｉｓｇｅｔｔｉｎｇｂｅｔｔｅｒ,ａｎｄａｆｔｅｒａｍｅｄｉｕｍｏｒｌｏｎｇｐｅ－ｒｉｏｄ（ｅ．ｇ,１０—２０ｙｅａｔｓ）,ｔｈｅＳＯＭｃｏｎｔｅｎｔｃａｎｇｅｔｔ     

pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用

观察ｐｐ６０ｃ－ｓｒｃ在血管紧张素Ⅱ（ＡｎｇⅡ）诱导血管平滑肌细胞（ＶＳＭＣｓ）内丝裂原活化蛋白激酶（ＭＡＰＫ）激活中的作用,以了解ＡｎｇⅡ促ＶＳＭＣｓ增殖的信号转导过程。将合成的反义ｃ－ｓｒｃ寡脱氧核苷酸（ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅ－ｏｔｉｄｅｓ,ＯＤＮｓ）以脂质体包裹转染培养的大鼠ＶＳＭＣｓ,用Ｗｅｓｔｅｒｎ印迹测得细胞裂解液中ｐｐ６０ｃ－ｓｒｃ含量明显下降,免疫沉淀方法测得ｐｐ６０ｃ－ｓ     

Dynamic Changes of β Tubulin during the Resumption of Meiosis of Mouse Oocyte

ＤｙｎａｍｉｃＣｈａｎｇｅｓｏｆβＴｕｂｕｌｉｎｄｕｒｉｎｇｔｈｅＲｅｓｕｍｐｔｉｏｎｏｆＭｅｉｏｓｉｓｏｆＭｏｕｓｅＯｏｃｙｔｅＬＩＵＨｕｉ;（刘辉）ＣＨＥＮＤａ－ｙｕａｎ;（陈大元）（ＳｔａｔｅＫｅｙＬａｏｂｏｒａｔｏｒｙｏｆＲｅｐｒｏｄｕｃｔｉ...     

杯状病毒的分类和分子生物学研究进展

杯状病毒的分类和分子生物学研究进展毛春生,金宁一,殷震（解放军农牧大学研究所,长春１３００６２）ＣｌａｓｓｉｆｉｃａｔｉｏｎａｎｄＭｏｌｅｃｕｌａｒＢｉｏｌｏｇｙｏｆＣａｌｉｃｉｖｉｒｕｓＭａｏＣｈｕｎｓｈｅｎｇ;ＪｉｎＮｉｎｇｙｉ;ＹｉｎＺｈｅｎ（...     

Regulation of C－Fos mRNA Expression in Sertoli Cells by cAMP，Ca~（＋＋），and protein Kinase C－mediated Pathways

ＲｅｇｕｌａｔｉｏｎｏｆＣ－ＦｏｓｍＲＮＡＥｘｐｒｅｓｓｉｏｎｉｎＳｅｒｔｏｌｉＣｅｌｌｓｂｙｃＡＭＰ，Ｃａ~（＋＋），ａｎｄｐｒｏｔｅｉｎＫｉｎａｓｅＣ－ｍｅｄｉａｔｅｄＰａｔｈｗａｙｓ￥ＪＩＡＭｅｎｇ－ｃｈｕｎ；（贾孟春）；ＮｅｅｌａｋａｎｔａＲ?..     

祁连山植被水文效应的多层次Fuzzy综合评判

祁连山植被水文效应的多层次Ｆｕｚｚｙ综合评判车克钧,傅辉恩（甘肃省张掖祁连山水源林研究所７３４０００）Ｍｕｌｔｉｐｌｅ－ＬｅｖｅｌＦｕｚｚｙＣｏｍｐｒｅｈｅｎｓｉｖｅＥｖａｌｕａｔｉｏｎｏｎＨｙｄｒｏｌｏｇｉｃａｌＦｕｎｃｔｉｏｎｓｏｆＶｅｇｅｔａｔｉｏｎｓｏｎｔｈｅＱｉｌｉａｎＭｏｕｎｔａｉｎｓ￥．ＣｈｅＫｅｊｕｎ;ＦｕＨｕｉｅｎ（ＱｉｌｉａｎＭｏｕｎｔａｉｎｓＩｎｓｔｉｔｕｔｅｏｆＦｏｒｅｓｔｆｏｒＷａｔｅｒＳｏｕｒｃｅＣｏｎｓｅｒｖａｔｉｏｎ,Ｚｈａｎｇｙｅ,ＧａｎｓｕＰｒｏｖｉｎｃｅ７３４０００）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（３）：３１－３５．ＢｙｍｅａｎｓｏｆｍｕＩｔｉｐｌｅ－ｌｅｖｅｌｆｕｚｚｙｃｏｍｐｒｅｈｅｎｓｉｖｅｅｖａｌｕａｔｉｏｎ,ｔｈｅｈｙｄｒｏｌｏｇｉｃａｌｆｕｎｃｔｉｏｎｓｏｆｆｏｕｒｖｅｇｅｔａｔｉｏｎｔｙｐｅｓａｒｅａｎａｌｙｚｅｄ．ＰｉｃｅａｃｒａｓｓｉｆｏｌｉａｆｏｒｅｓｔｉｓｓｈｏｗｎｔｏｂｅｔｈｅｂｅｓｔｔｙｐｅｆｏｒｗａｔｅｒｃｏｎｓｅｒｖａｔｉｏｎｏｎＱｉｌｉａｎｍｏｕｎｔａｉｎｓ,Ｓａｂｉｎａｐｒｚｅｗｌｓｋｉｉｆｏｒｅｓｔａ     

竹节花黄斑驳病毒启动了在棉花维管组织中是一个强启动子

用竹节花黄斑驳病毒（Ｃｏｍｍｅｌｉｎａ Ｙｅｌｌｏｗ Ｍｏｔｔｌｅ Ｖｉｒｕｓ,ＣｏＹＭＶ）启动子－ｇｕｓ嵌合基因和ＣａＭＶ３５Ｓ－ｇｕｓ嵌合基因通过根癌土壤杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ ｔｕｍｅｆａｃｉｅｎｓ（Ｓｍｉｔｈ ｅｔ Ｔｏｗｎｓｅｎｄ）Ｃｏｎｎ）ＬＢＡ４４０４介导分别转入棉花（Ｇｏｓｓｙｐｉｕｍ ｈｉｒｓｕｔｕｍ Ｌ．ｃｖ．Ｊｉｎｇｍｉａｎ７,Ｊ７Ｇ）,诱导再生了棉花转基因植株。Ｇ     

宁南山区灌溉农业立体复合种植生态系统生产力的研究

宁南山区灌溉农业立体复合种植生态系统生产力的研究杜守宇田恩平,王占山（宁夏农业技术推广总站,银川７５０００１）（固原县农业技术推广中心,７５６０００）ＳｔｕｄｙｏｎＰｒｏｄｕｃｔｉｖｉｔｙｏｆＩｒｒｉｇａｔｅｄＳｔｅｒｅｏｓｃｏｐｉｃＭｕｌｔｉｐｌｅ－ＣｒｏｐｐｉｎｇＥｃｏｓｙｓｔｅｍｓｉｎＭｏｕｎｔａｉｎｏｕｓＡｒｅａｏｆＳｏｕｔｈｅｒｎＮｉｎｇｘｉａ￥ＤｕＳｈｏｕｙｕ（ＣｅｌｌｅｒａｌＳｔａｔｉｏｎｏｆＡｇｒｉｃｕｌｔｕｒａｌＴｅｃｈｎｉｑｕｅＥｘｔｅｎｓｉｏｎｏｆＮｉｎｇｘｉａ,Ｙｉｎｃｈｕａｎ７５０００１）,ＴｉａｎＥｎｐｉｎｇ,ＷａｎｇＺｈａｎｓｈａｎ（ＡｇｒｉｃｕｌｔｕｒａｌＴｅｃｈｎｉｑｕｅＥｘｔｅｎｓｉｏｎＣｅｎｔｅｒｏｆＣｕｙｕａｎｃｏｕｎｔｙ,７５６０００）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３．１２（６）：１１－１６．ＴｈｅｖａｒｉｏｕｓｃｒｏｐｐｉｎｇｅｃｏｓｙｓｔｅｍｓａｒｅａｓｓｅｓｓｅｄｃｏｍｐｒｅｈｅｎｓｉｖｅｌｙｆｏｒｂｅｎｅｆｉｔｂｙｔｈｅａｎａｌｙｓｉｓｏｆＨｉｅｒａｒｃｈｉｃａｌｐｒｏｃｅｄｕｒｅａｎｄｔｈｅｅｘｐｅｃｔｅｄｂｅｎｅｆｉｃｉａｌｖａ     

贵州蓑藓属一新种

ＴｈｅｒｅａｒｅｓｐｅｃｉｅｓｏｆＭａｃｒｏｍｉｔｒｉｕｍｉｎＧｕｉｚｈｏｕｐｒｏｖｉｎｃｅＣｈｉｎａ .Ａｍｏｎｇｔｈｅｍ ,2ｓｐｅｃｉｅｓａｒｅｅｎ ｄｅｍｉｃｓｐｅｃｉｅｓ ,Ｍ .ｃａｖａｌｅｒｉｅｒＣａｒｄ＆Ｔｈｅｒ .ａｎｄＭ .ｆｏｒｔｕｎａｔｉｉＣａｒｄ＆Ｔｈｅｒ .Ｄｕｒｉｎｇａｒｅｃｅｎｔｒｅ -ｅｘａｍｉｎａｔｉｏｎｏｆｓｏｍｅＭａｃｒｏｍｉｔｒｉｕｍｓｐｅｃｉｍｅｎｓｆｒｏｍｔｈｉｓｒｅｇｉｏｎ .ＡｔａｘｏｎｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｓｐｅｃｉｅｓｏｆｔｈｅＭ .ｆｅｒｒｉｅｉＣａｒ…     

氟监测植物的筛选及监测指标的研究

本文报道用开顶式熏气装置筛选大气氟监测植物,研究监测植物在氟污染情况下的反应,以确立监测指标和监测定量化。结果表明,梅、唐菖蒲、赤胫散、火炭母、水杉、金荞麦、雪松、苹果等植物是良好的氟监测植物,其中赤胫散、火炭母是国内筛选出来的监测植物,可用来监测大气氟污染。受害浓度(阈值)、症状出现时间、受害叶面积％和叶片含氟量可作为植物的监测指标,生理反应指标作为参考。     

Peculiarities of fluoride accumulation in the plants of meadow biotopes of Northern Bukovyna

The performed studies of fluoride content in the system “soil-plant” have revealed the regularities of fluoride absorption by herbaceous plants of meadow biotopes of Northern Bukovyna. The ecological groups of plants with different level of fluoride absorption were determined according to the complex of criteria. A plant-concentrator Chamaerion angustifolium (L.) Holub was singled out.     

美洲斑潜蝇的寄主植物种类、适合度及其为害性的评价

在山西省调查结果,美洲斑潜蝇的寄生有２９种栽培植物,１１种野生植物,它们分属１６个科,其中以葫芦科,茄科和豆科的种类居多。应用选择系数法和适合性指数法定量测定了１６种常见寄生对美洲斑潜蝇的适合度,建立了选择强度与适合度估测模。根据适合度将美洲斑潜蝇的寄生植物划分为３个类型,即适宜寄主、较适宜寄主和次要寄主,在上述研究的基础上,测定了该在不同寄主上的虫道面积和蛹的重量,明确了不同寄主植物对害虫发育的     

Hydrogen fluoride effects on plasma membrane composition,ATPase activity and cell structure in needles of eastern white pine (Pinus strobus) seedlings

Eastern white pine (Pinus strobus L.) seedlings were grown in controlled environment growth cabinets and fumigated with 0.4 and 1.6 g m^–3 hydrogen fluoride for 2–28 days. Plasma membranes were isolated from needles of treated and control seedlings and their chemical composition and ATPase activity examined to determine early effects of hydrogen fluoride action. In plants treated for 2 days with both fluoride levels, ratios of plasma membrane free sterols:phospholipids and sterols:proteins were drastically higher than ratios in control plants. Seedlings treated with hydrogen fluoride for 8 days contained plasma membranes with elevated phospholipid:protein and sterol:protein ratios and their plasma membrane ATPase activity was higher than that of control plants. Prolonged, 28-day hydrogen fluoride treatment with 1.6 g m^–3 level was the only treatment which produced a drastic inhibition of plasma membrane ATPase activity. During the initial stages of hydrogen fluoride treatment, treated cells did not show alterations of ultrastructure which were previously shown in cells of plants treated with soil applied sodium fluoride. The results of the present study indicate that the plasma membranes may be among the initial sites of hydrogen fluoride injury to plants as well as initial sites of defense reaction.     

Removal of Fluoride and Arsenic by a Hybrid Constructed Wetland System

A pilot‐scale hybrid wetland system was constructed for the removal of fluoride and arsenic from synthetic wastewater. After five months of operation, the fluoride and arsenic removal rate were at the value of 65 % and 90 %, respectively. Through calculation, the accumulation of fluoride in plants only accounted for 1.63 % of the accumulation in substrates, and the accumulation of arsenic in plants accounted for 3.3 % of that in substrates. Both the accumulation of fluoride and arsenic in plants were much higher in roots than that in leaves. And for substrates, the accumulation in the first layer was higher than the second layer. The changes of microbial community in the substrate of the wetland during the operation were also analyzed to investigate the effects of operating condition on the microbial community and to study the role of microorganism on the removal of fluoride and arsenic. The results showed that the relative abundance of Firmicutes reduced, while the relative abundance of Proteobacteria increased, indicating that the fluoride and arsenic in solution had a great influence on the microbial community. Findings of this study suggest that the hybrid constructed wetland system may be a promising process for the removal of fluoride and arsenic from synthetic wastewater.     

肇庆市区道路绿化带植物景观评价研究

采用描述因子法,选择若干对道路植物景观效果贡献较大的指标,建立了整体指标评价体系。采用景观综合评价指数法模型,通过对指标的量化,对肇庆市道路植物景观进行了评价与分析。结果表明,肇庆市道路植物景观整体建设水平较好,55%的道路植物景观评价结果在Ⅱ级以上,仅有5%的道路在Ⅳ级以下。在所评价的8个因子中得分最高的是观赏时序,其次是观赏特性,这与所选的树种有很大关系;得分最低的是物种多样性,这与城市道路植物景观的高度人工化有关。针对评价结果提出了植物景观要从生态的可持续发展上体现它的价值、因地制宜地增加植物多样性及建立科学的绿地系统管理机制等建议。     

转基因植物环境安全评价策略

构建完善的转基因植物环境安全评价技术体系是保障转基因生物产业健康发展的重要组成部分。本文综述了转基因植物环境安全评价技术发展历程与趋势,归纳了转基因植物环境安全评价的思路与内容。转基因植物环境安全评价应分为潜在风险分析、风险假设验证、风险特征描述等3个步骤,并采用逐层评价模式;安全评价应贯穿转基因植物新品种研发与产业化全程,包括应用前预测、研发中筛选、推广前评价、推广后监测。此外,基于科学性和个案分析原则,本文对复合性状、非生物胁迫抗性等新型转基因植物环境安全评价策略进行了探讨。     

佛山市几种绿地植物氟化物时空累积特征

对佛山市的防护绿地、公园绿地、生产绿地及附属绿地等城市绿地系统中的大叶榕、细叶榕、芒果、白兰、垂叶榕和尖叶杜英的叶片和各绿地系统中的大气氟化物含量进行了测定,并对其相互关系进行了研究。结果表明:四季中各绿地系统的大气氟化物含量均值的大小依次为防护绿地的工业区、交通区、附属绿地、生产绿地和公园绿地;工业区的大气氟化物浓度约为公园绿地的3.4倍,不同城市绿地系统的1、2年生植物叶片含氟量均随时间的延长而逐渐增加;大气氟化物与叶片含氟量之间存在密切相关性,反映了植物氟化物的空间累积特征,其中细叶榕和垂叶榕的1年生和2年生叶片、芒果的1年生叶片、白兰的2年生叶片中的氟化物浓度能较好地表征城市绿地系统中大气氟化物的浓度。     

Green fluorescent protein as a visual marker in somatic hybridization

Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants. It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.     

Investigation of the reaction of magnesium with p-nitro-o-carboxylazochromotropic acid and the determination of magnesium in plants and in water

The formation of a novel complex between magnesium and p-nitro-o-carboxylazochromotropic acid (NCBAC) has been observed which is sensitive and selective at pH 10.4 in the presence of ethylenediamine and fluoride. The constants of formation of this complex have been determined by a spectral correction technique, and the complexation reaction has been applied to the spectrophotometric determination of trace magnesium in plants and in water with satisfactory results.