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1.
Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers 总被引:43,自引:0,他引:43
T. Nagaoka Y. Ogihara 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):597-602
Inter-simple sequence repeat polymorphic DNA (ISSR) was evaluated for its applicability as a genetic marker system in wheat.
PCR was carried out with primers that annealed to simple sequence repeats. The resultant products were subjected to agarose-gel
electrophoresis, and the banding patterns were compared among six wheat accessions containing diploid, tetraploid, and hexaploid
members. Out of 100 examined, 33 primers produced distinguishable as well as polymorphic bands in each of the six accessions.
Although most of the primers that gave distinct bands (30 primers out of 33) contained dinucleotide repeats, each of the primers
with tri-, tetra-, and penta-nucleotide motifs also yielded discrete bands. Primers based on (AC)n repeats gave the most polymorphic bands. In total, 224 polymorphic bands were found in the comparison between Einkorn wheats
whereas, on the average, 120 polymorphic bands were detected between common wheats. ISSR primers produced several times more
information than RAPD markers. The extent of band polymorphism was similar to that of RFLP markers, and greater than that
of RAPDs. The genetic relationships of wheat accessions estimated by the polymorphism of ISSR markers were identical with
those inferred by RFLP and RAPD markers, indicating the reliability of ISSR markers for estimation of genotypes. These polymorphic
bands are potential candidates as novel markers for use in linkage-map construction in wheat. The characteristic features
of ISSR markers, i.e. polymorphism, generation of information and ease of handling, suggest their applicability to the analysis
of genotypes as well as to the construction of PCR-based genome maps of wheats.
Received: 15 September 1996 / Accepted: 25 October 1996 相似文献
2.
Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map 总被引:25,自引:0,他引:25
A. A. Sankar G. A. Moore 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):206-214
Inter-simple sequence repeat (ISSR) analysis was evaluated for its usefulness in generating markers to extend the genetic
linkage map of Citrus using a backcross population previously mapped with restriction fragment length polymorphism (RFLP), random amplified polymorphic
DNA (RAPD) and isozyme markers. ISSR markers were obtained through the simple technique of PCR followed by analysis on agarose
gels, using simple sequence repeat (SSR) primers. Optimization of reaction conditions was achieved for 50% of the SSR primers
screened, and the primers amplified reproducible polymorphic bands in the parents and progeny of the backcross population.
Mendelian segregation of the polymorphic bands was demonstrated, with an insignificant number of skewed loci. Most of the
SSR primers produced dominant loci; however co-dominance was observed with loci derived from three primers. A new genetic
map was produced by combining the segregation data for the ISSR markers and data for the RFLP, RAPD and isozyme markers from
the previous map and creating genetic linkages among all the markers using JoinMap 2.0 mapping software. The new map has an
improved distribution of markers along the linkage groups with fewer gaps, and marker order showed partial or complete conservation
in the linkage groups. The incorporation of ISSR markers into the genetic linkage map demonstrates that ISSR markers are suitable
for genetic mapping in Citrus.
Received: 3 February 2000 / Accepted: 12 May 2000 相似文献
3.
I. Eujayl M. Baum W. Powell W. Erskine E. Pehu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):83-89
A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred
lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of
6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod
indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion.
More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion
than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the
most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification
of markers linked to quantitative trait loci in this population.
Received: 6 November 1997 / Accepted: 10 February 1998 相似文献
4.
Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population 总被引:18,自引:0,他引:18
M. Maheswaran P. K. Subudhi S. Nandi J. C. Xu A. Parco D. C. Yang N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):39-45
We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution
and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs
of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed
over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers
to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with
AFLP markers they will be very useful for marker-assisted backcrossing.
Received: 11 April 1996 / Accepted: 14 June 1996 相似文献
5.
Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides 总被引:11,自引:0,他引:11
G. L. Sun T. Fahima A. B. Korol T. Turpeinen A. Grama Y. I. Ronin E. Nevo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):622-628
The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers
flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations
revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic
RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating
F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum-likelihood technique it was found that the order OPB13
1420
–Yr15–Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between
OPB13
1420
–Yr15–Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for
the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.
Received: 27 February 1997/Accepted: 7 March 1997 相似文献
6.
Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers 总被引:12,自引:0,他引:12
C. Maliepaard F. H. Alston G. van Arkel L. M. Brown E. Chevreau F. Dunemann K. M. Evans S. Gardiner P. Guilford A. W. van Heusden J. Janse F. Laurens J. R. Lynn A. G. Manganaris A. P. M. den Nijs N. Periam E. Rikkerink P. Roche C. Ryder S. Sansavini H. Schmidt S. Tartarini J. J. Verhaegh M. Vrielink-van Ginkel G. J. King 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):60-73
Linkage maps for the apple cultivars ‘Prima’ and ‘Fiesta’ were constructed using RFLP, RAPD, isozyme, AFLP, SCAR and microsatellite
markers in a ‘Prima’בFiesta’ progeny of 152 individuals. Seventeen linkage groups, putatively corresponding to the seventeen
haploid apple chromosomes, were obtained for each parent. These maps were aligned using 67 multi-allelic markers that were
heterozygous in both parents. A large number of duplicate RFLP loci was observed and, in several instances, linked RFLP markers
in one linkage group showed corresponding linkage in another linkage group. Distorted segregation was observed mainly in two
regions of the genome, especially in the male parent alleles. Map positions were provided for resistance genes to scab and
rosy leaf curling aphid (Vf and Sd
1, respectively) for the fruit acidity gene Ma and for the self-incompatibility locus S. The high marker density and large number of mapped codominant RFLPs and some microsatellite markers make this map an ideal
reference map for use in other progenies also and a valuable tool for the mapping of quantitative trait loci.
Received: 17 November 1997 / Accepted: 9 December 1997 相似文献
7.
Combined mapping of AFLP and RFLP markers in barley 总被引:56,自引:0,他引:56
Jörg Becker Pieter Vos Martin Kuiper Francesco Salamini Manfred Heun 《Molecular & general genetics : MGG》1995,249(1):65-73
AFLP marker technology allows efficient DNA fingerprinting and the analysis of large numbers of polymorphic restriction fragments on polyacrylamide gels. Using the doubled haploids from the F1 of the cross Proctor × Nudinka, 118 AFLP markers were mapped onto a barley (Hordeum vulgare L.) RFLP map, also including five microsatellite and four protein marker loci. The AFLP markers mapped to all parts of the barley chromosomes and filled in the gaps on barley chromosomes 2L, 4L and 6 in which no RFLP loci had been mapped. Interestingly, the AFLP markers seldom interrupted RFLP clusters, but grouped next to them. The combined map covers 1873 cM, with a total of 282 markers. The merging of AFLP and RFLP markers increased the total map length; 402 cM were added to the map at the tips of chromosomes or in regions corresponding to earlier gaps. Another 375 cM resulted from mapping AFLP markers near to RFLP clusters or in between non-clustered RFLP markers. 相似文献
8.
Utility of RAPD markers in identifying genetic linkages to genes of economic interest in peach 总被引:7,自引:0,他引:7
M. L. Warburton V. L. Becerra-Velásquez J. C. Goffreda F. A. Bliss 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(5-6):920-925
The identification of molecular markers linked to economically important traits for use in crop improvement is very important in long-lived perennial species. Three-hundred-and-sixty RAPD primers were used with bulked segregant analysis to identify markers linked to loci of specific interest in peach [(Prunus persica) L. Batch] and peach x almond [(Prunus dulcis) Batch] crosses. The traits analyzed included flesh color, adhesion, and texture; pollen fertility; plant stature; and three isozyme loci. The Mendelian behavior of the RAPD loci was established, and RAPD markers were mapped relative to the loci controlling flesh color, adhesion, and texture, and the isozyme loci Mdh-1, 6Pgd-2 and Aat-1, as well as the existing RFLP genetic linkage map constructed previously using a peach x almond F2 population. This technique has facilitated rapid identification of RAPD and RFLP markers that are linked to the traits under study. Loci controlling these traits mapped predominantly to linkage groups 2 and 3 of the peach genetic linkage map. Linkages to genes with both dominant and co-dominant alleles were identified, but linkages to dominant genes were more difficult to find. In several crosses, RAPD marker bands proved to be allelic. One co-dominant RAPD formed a heteroduplex band in heterozygous individuals and in mixtures of alternate homozygotes. The Mendelian behavior of the RAPD loci studied was established and the results suggest that RAPD markers will be useful for plant improvement in peach. 相似文献
9.
Identification of RAPD markers linked to a locus involved in quantitative resistance to TYLCV in tomato by bulked segregant analysis 总被引:8,自引:0,他引:8
V. Chagué J. C. Mercier M. Guénard A. de Courcel F. Vedel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):671-677
In tomato, Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a quantitative
trait locus (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Virus. F4 lines were distributed into two pools, each consisting of the most resistant and of the most susceptible individuals, respectively.
Both pools were screened using 600 random primers. Four RAPD markers were found to be linked to a QTL responsible for up to
27.7% of the resistance. These markers, localized in the same linkage group within a distance of 17.3 cM, were mapped to chromosome
6 on the tomato RFLP map.
Received: 21 August 1996 / Accepted: 4 April 1997 相似文献
10.
V. Laporte D. Merdinoglu P. Saumitou-Laprade G. Butterlin P. Vernet J. Cuguen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):989-996
The present study shows that the recently described mitochondrial H haplotype is associated with cytoplasmic male-sterility
(CMS). This new source of CMS appears to be different from the mitotype E-associated CMS most frequently found in natural
populations. A mitotype H progeny with a sexual phenotype segregation was used to identify a gene restoring male fertility
(R1H ). Using bulk segregant analysis (BSA), nine RAPD markers linked to this restorer locus were detected and mapped. The comparison
with other Beta genetic maps shows that the closest RAPD marker, distant from R1H by 5.2 cM, belongs to the same linkage group as the monogermy locus. In order to determine the position of R1H more precisely, four RFLP loci within this linkage group were mapped in the segregating progeny. It thus became possible
to construct a linkage map of the region containing the RFLP, RAPD and R1H loci. The closest RFLP marker was located 1.7 cM away from R1H. However, a nuclear gene restoring the ‘Owen’ CMS which is currently used in sugar beet breeding is reportedly linked to
the monogermy locus, raising the question of a possible identity between the new CMS system and the ‘Owen’ CMS.
Received: 15 September 1997 / Accepted: 1 December 1997 相似文献
11.
Identification of molecular markers in soybean comparing RFLP,RAPD and AFLP DNA mapping techniques 总被引:16,自引:0,他引:16
Jhy-Jhu Lin Jonathan Kuo Jin Ma James A. Saunders Hunter S. Beard Margaret H. MacDonald William Kenworthy George N. Ude Benjamin F. Matthews 《Plant Molecular Biology Reporter》1996,14(2):156-169
Three different DNA mapping techniques—RFLP, RAPD and AFLP—were used on identical soybean germplasm to compare their ability
to identify markers in the development of a genetic linkage map. Polymorphisms present in fourteen different soybean cultivars
were demonstrated using all three techniques. AFLP, a novel PCR-based technique, was able to identify multiple polymorphic
bands in a denaturing gel using 60 of 64 primer pairs tested. AFLP relies on primers designed in part on sequences for endonuclease
restriction sites and on three selective nucleotides. The 60 diagnostic primer pairs tested for AFLP analysis each distinguished
on average six polymorphic bands. Using specific primers designed for soybean fromEco RI andMse I restriction site sequences and three selective nucleotides, as many as 12 polymorphic bands per primer could be obtained
with AFLP techniques. Only 35% of the RAPD reactions identified a polymorphic band using the same soybean cultivars, and in
those positive reactions, typically only one or two polymorphic bands per gel were found. Identification of polymorphic bands
using RFLP techniques was the most cumbersome, because Southern blotting and probe hybridization were required. Over 50% of
the soybean RFLP probes examined failed to distinguish even a single polymorphic band, and the RFLP probes that did distinguish
polymorphic bands seldom identified more than one polymorphic band. We conclude that, among the three techniques tested, AFLP
is the most useful. 相似文献
12.
Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping 总被引:11,自引:0,他引:11
S. Nandi P. K. Subudhi D. Senadhira N. L. Manigbas S. Sen-Mandi N. Huang 《Molecular & general genetics : MGG》1997,255(1):1-8
By combining the amplified fragment length polymorphism (AFLP) technique with selective genotyping, we constructed a linkage
map for rice and assigned each linkage group to a corresponding chromosome. The AFLP map, consisting of 202 AFLP markers,
was generated from 74 recombinant inbred lines (RIL) which were selected from both extremes of the population (250 lines)
with respect to the response to complete submergence. Map length was 1756 cM, with an average interval size of 8.5 cM. To
assign linkage groups to chromosomes, we used 50 previously mapped AFLP markers as anchor markers distributed over the 12
chromosomes. Other AFLP markers were then assigned to specific chromosomes based on their linkage to anchor markers. This
AFLP map is equivalent to the RFLP/AFLP map constructed previously as the anchors were in the same order in both maps. Furthermore,
tests with two restriction fragment length polymorphism (RFLP) markers and two sequence-tagged site (STS) markers showed that
they mapped in the expected positions. Using this AFLP map, a major gene for submergence tolerance was localized on chromosome
9. Quantitative trait loci (QTL) associated with submergence tolerance were detected on chromosomes 6, 7, 11, and 12. We conclude
that the combination of AFLP mapping and selective genotyping provides a much faster and easier approach to QTL identification
than the use of RFLP markers.
Received: 20 December 1996 / Accepted: 21 January 1997 相似文献
13.
Genetic linkage map in sour cherry using RFLP markers 总被引:6,自引:0,他引:6
D. Wang R. Karle T. S. Brettin A. F. Iezzoni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1217-1224
Restriction fragment length polymorphism (RFLP) linkage maps of two tetraploid sour cherry (Prunus cerasus L., 2n=4x=32) cultivars, Rheinische Schattenmorelle (RS) and Erdi Botermo (EB), were constructed from 86 progeny from the cross RS×EB.
The RS linkage map consists of 126 single-dose restriction fragment (SDRF, Wu et al. 1992) markers assigned to 19 linkage
groups covering 461.6 cM. The EB linkage map has 95 SDRF markers assigned to 16 linkage groups covering 279.2 cM. Fifty three
markers mapped in both parents were used as bridges between both maps and 13 sets of homologous linkage groups were identified.
Homoeologous relationships among the sour cherry linkage groups could not be determined because only 15 probes identified
duplicate loci. Fifty nine of the markers on the linkage maps were detected with probes used in other Prunus genetic linkage maps. Four of the sour cherry linkage groups may be homologous with four of the eight genetic linkage groups
identified in peach and almond. Twenty one fragments expected to segregate in a 1 : 1 ratio segregated in a 2 : 1 ratio. Three
of these fragments were used in the final map construction because they all mapped to the same linkage group. Six fragments
exhibited segregation consistent with the expectations of intergenomic pairing and/or recombination.
Received: 1 April 1998 / Accepted: 9 June 1998 相似文献
14.
Genetic variability of the wild diploid wheat Triticum urartu revealed by RFLP and RAPD markers 总被引:4,自引:0,他引:4
R. Castagna S. Gnocchi M. Perenzin M. Heun 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(3-4):424-430
Genetic variability among 49 accessions of Triticum urartu was estimated by RFLP and RAPD marker analyses, and the two data sets were compared. One T. timopheevii accession and two accessions of T. durum and T. aestivum, respectively, were included to identify T. urartu accessions closely related to these polyploid wheats. Twenty eight RFLP clones and 29 RAPD primers generated 451 and 155
polymorphic bands, respectively. The three accessions from Armenia clustered together and were well separated from all other
accessions, which showed less pronounced geographical patterns. Genetic similarity and co-phenetic values calculated with
RAPD markers were very similar to those calculated with RFLP markers for the intraspecific comparisons, but not for the interspecific
comparisons. The identification of individual T. urartu accessions which are more related to polyploid wheats than others was not possible.
Received: 14 May 1996 / Accepted: 13 September 1996 相似文献
15.
C. Rameau D. Dénoue F. Fraval K. Haurogné J. Josserand V. Laucou S. Batge I. C. Murfet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):916-928
Random amplified polymorphic DNA (RAPD) markers linked to two morphological markers ( fa and det), three ramosus genes (rms2, rms3 and rms4) and two genes conferring flowering response to photoperiod in pea (sn, dne) were selected by bulk segregant analysis on F2 populations. Two RAPD fragments were cloned and sequenced to generate the two SCAR markers V20 and S2 which are linked to
rms3 and dne, respectively. All these genes, except rms2, were previously located on the pea classical linkage map. Rms2 mapped to linkage group IB which contains the afila gene. Precise genetic maps of the regions containing the genes were obtained and compared to the RAPD map generated from
the recombinant inbred-lines population of the cross Térèse×K586. This cross was chosen because several mutants were obtained
from cultivars Térèse and Torsdag (K586 was derived from Torsdag). This collection of isogenic lines was used for the construction
of F2 mapping populations in which polymorphic RAPD markers were already known and mapped. Moreover, the well-known problem in
pea of variability in the linkage associations between crosses was avoided. This work contributes to the precise integration
between the classical map and the molecular maps existing in pea.
Received: 13 March 1998 / Accepted: 29 April 1998 相似文献
16.
Tenhola-Roininen T Kalendar R Schulman AH Tanhuanpää P 《Journal of applied genetics》2011,52(3):299-304
A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through
anther culture of F1 plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289
loci: amplified fragment length polymorphism (AFLP), microsatellite, random amplified polymorphic DNA (RAPD), retrotransposon-microsatellite
amplified polymorphism (REMAP), inter-retrotransposon amplified polymorphism (IRAP), inter-simple sequence repeat (ISSR) and
sequence-related amplified polymorphism (SRAP) markers, and extended altogether 732 cM (one locus in every 2.5 cM). All of
the seven rye chromosomes and four unplaced groups were formed. Distorted segregation of markers (P ≤ 0.05) was detected on all chromosomes. One major quantitative trait locus (QTL) affecting α-amylase activity was found,
which explained 16.1% of phenotypic variation. The QTL was localized on the long arm of chromosome 5R. Microsatellites SCM74,
RMS1115, and SCM77, nearest to the QTL, can be used for marker-assisted selection as a part of a rye breeding program to decrease
sprouting damage. 相似文献
17.
Y. Loarce G. Hueros E. Ferrer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(7):1112-1118
A genetic map of six chromosomes of rye, (all of the rye chromosomes except for 2R), was constructed using 77 RFLP and 12 RAPD markers. The map was developed using an F2 population of 54 plants from a cross between two inbred lines. A rye genomic library was constructed as a source of clones for RFLP mapping. Comparisons were made between the rye map and other rye and wheat maps by including additional probes previously mapped in those species. These comparisons allowed (1) chromosome arm orientation to the linkage groups to be given, (2) the corroboration of several evolutionary translocations between rye chromosomes and homoeologous chromosomes of wheat; (3) an increase in the number of available markers for target regions of rye that show colinearity with wheat. Inconsistencies in the location of markers between the wheat and rye maps were mostly detected by multi-copy probes. 相似文献
18.
Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers
MAMTA GUPTA BHAWNA VERMA NARESH KUMAR RAKESH K. CHAHOTA RAJEEV RATHOUR SHYAM K. SHARMA SABHYATA BHATIA TILAK R. SHARMA 《Journal of genetics》2012,91(3):279-287
Lentil (Lens culinaris ssp. culinaris), is a self-pollinating diploid (2n?=?2x?=?14), cool-season legume crop and is consumed worldwide as a rich source of protein (~24.0%), largely in vegetarian diets. Here we report development of a genetic linkage map of Lens using 114 F2 plants derived from the intersubspecific cross between L 830 and ILWL 77. RAPD (random amplified polymorphic DNA) primers revealed more polymorphism than ISSR (intersimple sequence repeat) and SSR (simple sequence repeat) markers. The highest proportion (30.72%) of segregation distortion was observed in RAPD markers. Of the 235 markers (34 SSR, 9 ISSR and 192 RAPD) used in the mapping study, 199 (28 SSRs, 9 ISSRs and 162 RAPDs) were mapped into 11 linkage groups (LGs), varying between 17.3 and 433.8 cM and covering 3843.4 cM, with an average marker spacing of 19.3 cM. Linkage analysis revealed nine major groups with 15 or more markers each and two small LGs with two markers each, and 36 unlinked markers. The study reported assigning of 11 new SSRs on the linkage map. Of the 66 markers with aberrant segregation, 14 were unlinked and the remaining 52 were mapped. ISSR and RAPD markers were found to be useful in map construction and saturation. The current map represents maximum coverage of lentil genome and could be used for identification of QTL regions linked to agronomic traits, and for marker-assisted selection in lentil. 相似文献
19.
Mohd Arif N. W. Zaidi Y. P. Singh Qazi Mohd Rizwanul Haq U. S. Singh 《Plant Molecular Biology Reporter》2009,27(4):488-495
Shisham (Dalbergia sissoo) is one of the most preferred timber tree species of South Asia. Two DNA-based molecular marker techniques, intersimple sequence
repeat (ISSR) and random amplified polymorphism DNA (RAPD), were compared to study the genetic diversity in this species.
A total of 30 polymorphic primers (15 ISSR and 15 random) were used. Amplification of genomic DNA of 22 genotypes, using ISSR
analysis, yielded 117 fragments, of which 64 were polymorphic. Number of amplified fragments with ISSR primers ranged from
five to ten and varied in size from 180 to 1,900 bp. Percentage polymorphism ranged from 0 to 87.5. The 15 RAPD primers produced
144 bands across 22 genotypes, of which 84 were polymorphic. The number of amplified bands varied from five to 13, with size
range from 180 to 2,400 bp. Percentage polymorphism ranged from 0 to 100, with an average of 58.3 across. RAPD markers were
relatively more efficient than the ISSR assay. The mental test between two Jaccard’s similarity matrices gave r ≥ 0.90, showing very good fit correlation in between ISSR- and RAPD-based similarities. Clustering of isolates remained more
or less the same in RAPD and combined data of RAPD and ISSR. The similarity coefficient ranged from 0.734 to 0.939, 0.563
to 0.946, and 0.648 to 0.920 with ISSR, RAPD, and combined dendrogram, respectively. 相似文献
20.
R. Freyre P. W. Skroch V. Geffroy A.-F. Adam-Blondon A. Shirmohamadali W. C. Johnson V. Llaca R. O. Nodari P. A. Pereira S.-M. Tsai J. Tohme M. Dron J. Nienhuis C. E. Vallejos P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):847-856
Three RFLP maps, as well as several RAPD maps have been developed in common bean (Phaseolus vulgaris L.). In order to align these maps, a core linkage map was established in the recombinant inbred population BAT93×Jalo EEP558
(BJ). This map has a total length of 1226 cM and comprises 563 markers, including some 120 RFLP and 430 RAPD markers, in addition
to a few isozyme and phenotypic marker loci. Among the RFLPs mapped were markers from the University of California, Davis
(established in the F2 of the BJ cross), University of Paris-Orsay, and University of Florida maps. These shared markers allowed us to establish
a correspondence between the linkage groups of these three RFLP linkage maps. In total, the general map location (i.e., the
linkage group membership and approximate location within linkage groups) has been determined for some 1070 markers. Approaches
to align this core map with other current or future maps are discussed.
Received: 10 March 1998 / Accepted: 22 April 1998 相似文献