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1.
Antisera raised against apparently homogeneous whiting parvalbumin III have been found to recognize two non cross-reacting molecular species of parvalbumins. Aliquots of these antisera have been separately absorbed with two distinct parvalbumins from a near-related fish species, namely haddock parvalbumins II and III, and also with the homologous antigen. The immunochemical reactivities of absorbed and non-absorbed antisera toward parvalbumins from nine Gadidae species have been systematically explored by immunoelectrophoresis. The observed cross-reactions lead to distinguish two groups among Gadidae parvalbumins. So far this discrimination can be correlated with differences in amino-acid compositions, peptide maps and sequences which are known to characterize several protein members from each of the two groups. Using the same anti-whiting antisera, a tenuous common antigenic reactivity is shown between Gadidae and some Cyprinidae parvalbumins. 相似文献
2.
Single skinned muscle fibres (frog) have been submitted to double Ouchterlony immunodiffusion assays with antibodies directed against the two species of frog parvalbumins. The antigenic material which diffuses out of each fibre contains the two parvalbumins. Their presence in each cell is thus demonstrated. The amount of parvalbumins having diffused out of the fibre has been quantified. It corresponds to the parvalbumin content of the cell. This implies that these proteins are freely soluble in the muscle sarcoplasm. 相似文献
3.
The component I isolated from carp red muscle has been characterized as a true parvalbumin, fairly different from carp parvalbumins described so far. The protein is antigenically related to the parvalbumin III from pike, which belongs to the so called parvalbumin lineage alpha. Immunological investigations on the location of the various carp parvalbumins reveal genuine variation in the pattern of these proteins according to organ and type of muscular tissue. 相似文献
4.
J.M. Gillis A. Piront Cécile Gosselin-Rey 《Biochimica et Biophysica Acta (BBA)/General Subjects》1979,585(3):444-450
Single skinned muscle fibres (frog) have been submitted to double Ouchterlony immunodiffusion assays with antibodies directed against the two species of frog parvalbumin. The antigenic material which diffuses out of each fibre contains the two parvalbumins. Their presence in each cell is thus demonstrated. The amount of parvalbumins having diffused out of the fibre has been quantified. It corresponds to the parvalbumin content of the cell. This implies that these proteins are freely soluble in the muscle sarcoplasm. 相似文献
5.
G Hamoir N Gerardin-Otthiers 《Comparative biochemistry and physiology. B, Comparative biochemistry》1979,64(1):17-23
1. The protein composition of the sarcoplasm of Champsocephalus gunnari white muscle has been examined by ultracentrifugation and starch-gel electrophoresis. 2. The extracts have been fractionated by several methods in order to compare them more closely to similar extracts of other fish species and to isolate creatine kinase and the parvalbumins IV and V. 3. The creatine kinase does not appear to differ from other fish creatine kinases. Both parvalbumins are also very similar to other parvalbumins except that they are more easily oxidized than all the parvalbumins described so far. 相似文献
6.
Structural proteins of dogfish skeletal muscle. 总被引:1,自引:0,他引:1
As part of a study on the evolutionary aspects of control mechanisms, a number of structural muscle components from the Pacific dogfish (Squalus acanthias) are described. These include troponin, tropomyosin, actin, and myosin. Troponin (mol wt 108.000) was resolved into its constitutive subunits, repeated by a 20,500 mol wt fragment which binds 2 mol of Ca2+/mol with a KDiss of 0.91 mum, and an inhibitory component of 30,000 and a 58,000 component which are necessary for the calcium sensitivity of actomyosin ATPase. Tropomyosin and actin share many properties with their counterparts from higher vertebrates. Proteins similar to parvalbumins, i.e., the low molecular weight calcium-binding proteins widely distributed in fish, amphibians, and mammalian muscle, could be generated from troponin and its calcium-binding subunit by limited proteolysis. The appearance of immunological cross-reactivity and other similar features suggested some identity, but differences in the amino acid analysis exclude the possiblity that parvalbumins occur as breakdown products of troponin. The close relationship between parvalbumins and the calcium-binding subunit brings additional evidence that these proteins have arisen through divergent evolution. 相似文献
7.
Allergenic characteristics of purified parvalbumins from different fish species have not been thoroughly investigated. We revealed that purified parvalbumins from nine different fish species have identical IgE-reactivities and high cross-reactivities. We also showed that fish allergenicity is associated with the parvalbumin content of the fish species, rather than species-specific differences in the molecular characteristics of the individual parvalbumin proteins. 相似文献
8.
江西省九岭山自然保护区鱼类资源概况 总被引:1,自引:1,他引:0
2007年10月对江西省九岭山自然保护区野生鱼类区系及资源进行了调查.共采集标本952尾,鉴定为39种,隶属于3目12科33属,多为山区溪流小型鱼类.以鲤科鱼类为主, 21种,占鱼类总物种数的53.85%.对各采样溪流进行多样性比较显示,青山站水系(H:3.2)多样性指数最高,和尚坪水系(H:1.9)多样性指数最低;各采样溪流的均匀性指数相差不大(E:0.7~0.9),且优势种群不明显(λ:0.1~0.3). 相似文献
9.
The ribosomal RNA gene expression in the genomes of evolutionary diploid (Scardinius erythrophthalmus, Leucaspius delineatus, Tinca tinca) and polyploid species (Cyprinus carpio, Carassius carassius, Carassius auratus gibelio, Carassius auratus auratus) of Cyprinidae has been investigated by means of a silver nitrate technique. The diploid species investigated exhibited only one pair of chromosomes with nucleolus organizers (NOR). Higher numbers of rRNA-expressing chromosomal sites in several evolutionary polyploid species (Carassins) gave evidence against a complete functional diploidization, at least with regard to the NOR bearing chromosomes in these species. The NORs displayed a heterochromatic brilliant chromomycin A3 fluorescence. No distamycin-A/DAPI-bright heterochromatic blocks were detected in the genomes of the Cyprinidae. 相似文献
10.
11.
E A Permyakov A V Ostrovsky E A Burstein P G Pleshanov C Gerday 《Archives of biochemistry and biophysics》1985,240(2):781-791
The binding of calcium to whiting (one tryptophan residue) and pike (one tyrosine residue) parvalbumins has been studied by means of kinetic and steady-state fluorescence techniques. The decay curves of the tryptophan and tyrosine fluorescence of the parvalbumins are best fitted by a sum of two exponents for any metal state of the proteins. The data can be interpreted as a nonexponential decay of the fluorescence of a single-type chromophore or in terms of equilibria between compact and relaxed conformers of the parvalbumins in each metal state. Fluorescence quenching by I-ions and effects of H2O/D2O substitution confirm the second interpretation. The constants of the equilibria have been evaluated. 相似文献
12.
《Fungal biology》2020,124(6):592-600
Parvalbumins play crucial physiological roles in neuromuscular systems of vertebrates, such as cell-cycle, development of neurons, contraction of muscles, and regulation of intracellular calcium. To perform these neuromuscular functions, parvalbumin may be in associated with other proteins including calbindin, carbonic anhydrase, and cytochrome oxidase. Humans may show an IgE-specific hypersensitivity to parvalbumins after consumption of some distinct fish species. While this protein is abundant in fish muscles, literature review of publications related to fish parvalbumins, do not point to the presence of parvalbumins in eukaryotic microbes. In this study, we propose that distantly related parvalbumins may be found in some non-fish species. Bioinformatics studies such as multiple sequence alignment (MSA), phylogenetic analysis as well as molecular-based experiments indicate that, at least two parvalbumins sequences (UniProt IDs: A0A178F775 and A0A178F7E4) with EF-hand domains and Ca2+-binding sites could be identified in Trichophyton violaceum, a pathogenic fungal species.It was determined that both genes consisted of a single exon and encoded for parvalbumin proteins possessing conserved amino acid motifs. Antigenicity prediction revealed antigenic sites located in both sides of the Ca2+-binding site of the first EF-hand domain. Our phylogenetic analysis revealed that one of parvalbumins (UniProt ID: 0A178F775) can be evolved to other parvalbumins in T. violaceum (UniProt ID: A0A178F7E4) and fish species through evolutionary phenomenon. To confirm our in-silico findings, we designed three primer pairs to detect one of the T. violaceum parvalbumins (UniProt ID: A0A178F7E4) by polymerase chain reaction (PCR); one primer pair showed a strong and specific band in agarose gel electrophoresis. To evaluate the specificity of the method, the primers were tested on extracted DNA from Trichophyton rubrum and T. mentagrophytes. The results demonstrated that the evaluated parvalbumin gene (UniProt ID: A0A178F7E4) was T. violaceum-specific and this pathogenic fungus can be differentiated from T. rubrum and T. mentagrophytes through identification of parvalbumin genes. Further studies are necessary to unravel the biochemical and physiological functions of parvalbumins in T. violaceum. 相似文献
13.
Crystal structure determination and refinement of pike 4.10 parvalbumin (minor component from Esox lucius) 总被引:1,自引:0,他引:1
The crystal and molecular structure of the minor component of pike parvalbumins has been determined at 1.93 A resolution by molecular replacement (1 A = 0.1 nm). The crystals are orthorhombic, space group P2(1)2(1)2 with a = 59.62 A, b = 59.83 A and c = 26.35 A. A location of the secondary cation binding site is proposed for this parvalbumin of the beta phylogenetic series. 相似文献
14.
Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers 总被引:3,自引:0,他引:3
The cyprinid genus Barbus, with more than 800 nominal species, is an apparently polyphyletic assemblage to which a number of unrelated species, groups and/or assemblages have been assigned. It includes species that exhibit three different ploidy levels: diploid, tetraploid and hexaploid. Several lineages of the family Cyprinidae constitute a major component of the African freshwater ichthyofauna, having about 500 species, and fishes assigned to the genus 'Barbus' have the most species on the continent. We used complete sequences of the mitochondrial cytochrome b gene in order to infer phylogenetic relationships between diploid, tetraploid and hexaploid species of 'Barbus' occurring in southern Africa, the only region where representatives of all of the three ploidy levels occur. The results indicate that most of the lineages are incorrectly classified in the genus 'Barbus'. The southern African tetraploids probably originated from southern African diploids. They constitute a monophyletic group distinct from tetraploids occurring in the Euro-Mediterranean region (Barbus sensu stricto). The 'small' African diploid species seem to be paraphyletic, while the 'large' African hexaploid barbs species are of a single, recent origin and form a monophyletic group. The evidence of multiple, independent origins of polyploidy occurring in the African cyprinine cyprinids thus provides a significant contribution to the knowledge on the systematic diversity of these fishes, and warrants a thorough taxonomic reorganization of the genus. 相似文献
15.
16.
One major parvalbumin each was isolated from the skeletal muscle of two
reptiles, a boa snake, Boa constrictor, and a map turtle, Graptemys
geographica, while two parvalbumins were isolated from an amphibian, the
salamander Amphiuma means. The amino acid sequences of all four
parvalbumins were determined from the sequences of their tryptic peptides,
which were ordered partially by homology to other parvalbumins.
Phylogenetic study of these and 16 other parvalbumin sequences revealed
that the turtle parvalbumin belongs to beta lineage, while the salamander
sequences belong, one each, to the alpha and beta lineages defined by
Goodman and Pechere (1977). Boa parvalbumin, however, while belonging to
the beta lineage, clusters within the fish in all reasonably parsimonious
trees. The most parsimonious trees show many parallel or back mutations in
the evolution of many parvalbumin residues, although the residues
responsible for Ca2+ binding are very well conserved. These most
parsimonious trees show an actinopterygian rather than a crossoptyrigian
origin of the tetrapods in both the alpha and beta groups. One of two
electric eel parvalbumins is evolving more than 10 times faster than its
paralogous partner, suggesting it may be on its way to becoming a
pseudogene. It is concluded that varying rates of amino acid replacement,
much homoplasy, considerable gene duplication, plus complicated lineages
make the set of parvalbumin sequences unsuitable for systematic study of
the origin of the tetrapods and other higher-taxa divergence, although it
may be suitable within a genus or family.
相似文献
17.
Merima Bublin Maria Kostadinova Julian E. Fuchs Daniela Ackerbauer Adolfo H. Moraes Fabio C. L. Almeida Nina Lengger Christine Hafner Christof Ebner Christian Radauer Klaus R. Liedl Ana Paula Valente Heimo Breiteneder 《PloS one》2015,10(11)
Fish allergy is associated with moderate to severe IgE-mediated reactions to the calcium binding parvalbumins present in fish muscle. Allergy to multiple fish species is caused by parvalbumin-specific cross-reactive IgE recognizing conserved epitopes. In this study, we aimed to produce cross-reactive single chain variable fragment (scFv) antibodies for the detection of parvalbumins in fish extracts and the identification of IgE epitopes. Parvalbumin-specific phage clones were isolated from the human ETH-2 phage display library by three rounds of biopanning either against cod parvalbumin or by sequential biopanning against cod (Gad m 1), carp (Cyp c 1) and rainbow trout (Onc m 1) parvalbumins. While biopanning against Gad m 1 resulted in the selection of clones specific exclusively for Gad m 1, the second approach resulted in the selection of clones cross-reacting with all three parvalbumins. Two clones, scFv-gco9 recognizing all three parvalbumins, and scFv-goo8 recognizing only Gad m 1 were expressed in the E. coli non-suppressor strain HB2151 and purified from the periplasm. scFv-gco9 showed highly selective binding to parvalbumins in processed fish products such as breaded cod sticks, fried carp and smoked trout in Western blots. In addition, the scFv-gco9-AP produced as alkaline phosphatase fusion protein, allowed a single-step detection of the parvalbumins. In competitive ELISA, scFv-gco9 was able to inhibit binding of IgE from fish allergic patients’ sera to all three β-parvalbumins by up to 80%, whereas inhibition by scFv-goo8 was up to 20%. 1H/15N HSQC NMR analysis of the rGad m 1:scFv-gco9 complex showed participation of amino acid residues conserved among these three parvalbumins explaining their cross-reactivity on a molecular level. In this study, we have demonstrated an approach for the selection of cross-reactive parvalbumin-specific antibodies that can be used for allergen detection and for mapping of conserved epitopes. 相似文献
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19.
哈拉哈河上游的鱼类区系和资源现状 总被引:1,自引:0,他引:1
哈拉哈河上游的鱼类采集到14种和亚种,分水于4目6科,其中鲤科8种,占57.1%。区系组成分归5个区系类群,其中属于全北区的北方山地,北方平原和北极淡水类群鱼类有10种,占71.4%,这与该河系具有适于冷水性鱼类栖存的生态条件密切相关。 相似文献
20.
The possible structural changes of the two major isotypes (PA1 and PA2) of parvalbumins from bullfrog (Rana catesbeiana) skeletal muscle caused by Ca2+ binding have been analyzed by microcalorimetric titrations. Titrations of the parvalbumins with Ca2+ have been made in both the absence and presence of Mg2+ at pH 7.0 and at 5, 15, and 25 degrees C. The reactions of the parvalbumins with Ca2+ are exothermic in both the presence and absence of Mg2+ and at every temperature. But the contributions of enthalpy and entropy changes are variable; Mg2+-Ca2+ exchange on PA1 at 25 degrees C is driven almost entirely by a favorable enthalpy change, whereas Ca2+ binding to PA2 at 5 degrees C is driven for the most part by a favorable entropy change. The magnitudes of the hydrophobic and internal vibrational contributions to the heat capacity and entropy changes of the parvalbumins on Ca2+ binding and Mg2+-Ca2+ exchange have been estimated by the empirical method of Sturtevant [Sturtevant, J. M. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 2236-2240]. Although PA1 (beta) and PA2 (alpha) belong to genetically different lineages, the parvalbumins indicate very similar conformational changes to each other on both Ca2+ binding and Mg2+-Ca2+ exchange. On Mg2+-Ca2+ exchange, the vibrational as well as hydrophobic entropy is slightly increased in a parallel manner. In contrast, on Ca2+ binding, the hydrophobic entropy increases but the vibrational entropy decreases. The increase in the hydrophobic entropy indicates the sequestering of nonpolar groups from the surface to the interior of molecules, while the changes in the vibrational entropy suggest that the overall structures are tightened on Ca2+ binding but loosened on Mg2+-Ca2+ exchange. 相似文献