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1.
Ayman El-Faham Abdel Moneim El Massry Adel Amer Yousry M. Gohar 《Letters in Peptide Science》2002,9(1):49-54
The synthesis of N-protected L-amino acid (3-benzylquinoxalin-2-yl) hydrazide derivatives is reported here. 3-Benzyl-2-hydrazinoquinoxaline was prepared and then coupled with N-Boc-L-amino acids including; Alanine, Valine, Leucine, Phenylalanine, Tyrosine, Serine and Proline in the presence of HBTU as a coupling reagent to provide the expected product with high yield and purity. The products were deprotected by p-toluenesulphonic acid in acetonitrile and then the tosylate salts were evaluated for antibacterial and antifungal activity. 相似文献
2.
Camarero Julio A. Adeva Alberto Muir Tom W. 《International journal of peptide research and therapeutics》2000,7(1):17-21
Summary This paper describes a practical new use of 3-mercaptopropionic acid as a highly versatile multidetachable linker for solid-phase
synthesis. Our approach is based on the stability of the alkylthioester functionality to optimized Boc-SPPS protocols and
HF treatment, as well as on the mild activation of the thioester functionality toward nucleophilic or reductive displacement.
This allows several C-terminal modifications to be introduced into a synthetic molecule during the cleavage step. We have
shown that unprotected peptides can be efficiently cleaved from a propyl thioester-polyethylene glycol-poly-(N,N-dimethylacrylamide) copolymer resin using a great variety of nucleophiles to give the corresponding C-terminally modified
peptides (esters, thioesters, carboxylic acids, thioacids, amides, hydroxamic acids, hydrazides, alcohols). The nucleophilic
cleavage reaction is both rapid and exceptionally clean in all the cases tested.
Abbreviations: HBTU,N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium hexafluorophosphateN-oxide); DIEA,N,N-diisopropylethylamine; DMF,N,N-dimethyl formamide; ES-MS, electrospray mass spectrometry; FAB-MS, fast atom bombardment mass spectrometry; HMBA, hydroxymethylbenzoic
acid; HPLC, high performance liquid chromatography; PBS: phosphate buffer saline; PEGA, polyethylene glycolpoly-(N,N-dimethylacrylamide); TFA, trifluoroacetic acid; SPPS, solid-phase peptide synthesis. Standard IUPAC single and triple letter
codes for amino acids are used throughout 相似文献
3.
Khattab Sh. N. El-Faham A. El-Massry A. M. Mansour E. M. E. Abd El-Rahman M. M. 《International journal of peptide research and therapeutics》2000,7(6):331-345
Summary The IR studies for the preactivation step of N-protected iminodiacetic acid with different coupling reagents (TCFH, TFFH,
HATU, HBTU, HSTU) were reported here and showed the formation of an anhydride as an active intermediate in case of TCFH and
TFFH. The formation of a mixture of an anhydride and an active ester (-OBt,-OAt or-OSu) were observed for HBTU, HATU or HSTU
coupling reagent. Dependent on the coupling conditions, acylation of N-protected iminodiacetic acid with amino acid ester
or amide derivatives in solution phase gave monoor di-substituted iminodiacetic acid derivatives. Coupling of N-protected
iminodiacetic acid with an amino acid or peptide attached to a solid support (PAL-PEG-PS or Wang resin) gave only the monosubstituted
iminodiacetic acid derivatives.
Abbreviations: HBTU, N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium hexafluorophosphate N-oxide; Boc,t-butyloxycarbonyl; DCC, N,N′-dicyclohexylcarbodiimide; DIC, N,N′-diisopropylcarbodiimide; DIEA, diisopropylethylamine; HATU,
N-[(dimethylamino)-1H-1,2,3,-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate N-oxide; DMF,
N,N-dimethylformamide; Bsmoc, 1,1-dioxobenzo[b]thiophene-2-ylmethoxycarbonyl; Fmoc, 9-fluorenylmethyloxycarbonyl; HOAt, l-hydroxy-7-azabenzotriazole;
HOBt, l-hydroxybenzotriazol; IDA, iminodiacetic acid; HSTU, O-(succinimidyl)-tetramethyluronium hexafluorophosphate; TCFH;
1,1,3,3-tetramethyl-2-chloroformamidinium hexafluorophosphate; TFFH, 1,1,3,3-tetramethyl-2-fluoroformamidinium hexafluorophosphate;
TMS-Cl, trimethylchlorosilane. Amino acids and peptides are abbreviated and designated following the rules of the IUPAC-IUB
Commission of Biochemical Nomenclature (J. Biol. Chem., 247 (1972) 997). 相似文献
4.
David S. Wishart Leslie H. Kondejewski Paul D. Semchuk Brian D. Sykes Robert S. Hodges 《Letters in Peptide Science》1996,3(1):53-60
Summary A simple method is described for the facile synthesis of gramicidin S and six other analogs, using standard solidphase synthetic technology and a single solution-phase cyclization step. The peptides were purified to homogeneity and characterized by plasma desorption time-of-flight mass spectrometry and NMR spectroscopy. Complete 1H NMR assignments for all seven peptides (in aqueous solution) are presented. Unlike previous approaches, the presented method is simple, automatable, rapid (less than three days), high-yielding, requires no side-chain protection during cyclization, and appears to be generally applicable to the preparation of a variety of related head-to-tail cyclic peptides.Abbreviations Boc
t-butyloxycarbonyl
- BOP
benzotriazoyl N-oxytris(dimethylamino)phosphonium hexafluorophosphate
- Bzl
benzyl
- DCC
N,N-dicyclohexylcarbodiimide
- DCM
dichloromethane
- DIEA
N,N-diisopropylethylamine
- DMF
N,N-dimethylformamide
- DQF-COSY
double-quantum-filtered correlation spectroscopy
- DSS
2,2-dimethyl-2-silapentane-5-sulfonate, sodium salt
- EDAC
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
- HBTU
2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyl uronium hexafluorophosphate
- HOBt
1-hydroxybenzotriazole
- 4-MeBzl
4-methylbenzyl
- NHS
N-hydroxysuccinimide
- NOESY
nuclear Overhauser effect spectroscopy
- PAM
phenylacetamidomethyl (resin)
- RP-HPLC
reversed-phase high-performance liquid chromatography
- TFA
trifluoroacetic acid
- TOCSY
total correlation spectroscopy
- Tos
tosyl
- Troc
2,2,2-trichloroethylcarbamate. 相似文献
5.
Vivek K. Sharma Sunil K. Singh Kapil Bohra Chandra Shekhar Reddy L. Vinod Khatri Carl E. Olsen 《Nucleosides, nucleotides & nucleic acids》2013,32(5):256-272
Three LNA-based mercaptoacetamido-linked nonionic nucleoside dimers TL-S-T, T-S-TL , and TL-S-TL have been synthesized by HOBT and HBTU catalyzed condensation of silyl-protected 2-S-(thymidin-5?′-yl)mercaptoacetic acid or 2-S-(2?′-O,4?′-C-methylenethymidin-5?′-yl)mercaptoacetic acid with 3?′-amino-3?′-deoxy-5?′-O-DMT-2?′-O,4?′-C-methylenethymidine or with 3?′-amino-3?′-deoxy-5?′-O-DMT-β-thymidine followed by desilylation of the protected dimers. The 3?′-O-phosphoramidite derivative of one of the nucleoside dimers was successfully prepared by condensation with [P(-Cl)(-OCH2CH2CN)-N(iPr)2}] in DCM in the presence of N,N-diisopropylethylamine (DIPEA), which is a building block for the preparation of mercaptoacetamido-linked oligonucleotides of therapeutic applications. 相似文献
6.
Jan Lukszo Dale Patterson Fernando Albericio Steven A. Kates 《Letters in Peptide Science》1996,3(3):157-166
Summary Several side reactions have been detected for cysteine-containing peptides. During the synthesis ofC-terminal cysteine peptides, a base-catalyzed elimination of the sulfhydryl-protected side-chain to afford the dehydroalanine derivative followed by a nucleophilic addition to the alkene was observed. MALDI-TOF analysis was a useful analytical technique to determine this phenomenon.Abbreviations Acm
acetamidomethyl
- Boc
tert-butyloxycarbonyl
-
t-Bu
tert-butyl
- DBU
1,8-diazabicyclo[5.4.0]undec-7-ene
- DIEA
N,N-diisopropylethylamine
- DMF
N,N-dimethylformamide
- Fmoc
9-fluorenylmethyloxycarbonyl
- HATU
N-[(dimethylamino)-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphateN-oxide
- HBTU
N-[(1H-benzotriazol-1-yl)(dimethylamino)methylene]-N-methylmethanaminium hexafluorophosphateN-oxide
- HOAt
1-hydroxy-7-azabenzotriazole
- HOBt
1-hydroxybenzotriazole
- HPLC
high-performance liquid chromatography
- MALDI-TOF
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- PAC
4-hydroxymethylphenoxyacetic acid handle
- PAL
5-(4-(9-fluorenylmethyloxycarbonyl)aminomethyl-3,5-dimethoxy-phenoxy)valeric acid handle
- PEG-PS
polyethylene glycol-polystyrene graft supports
- PS
polystyrene
- Reagent R
TFA/thioanisole/1,2-ethanedithiol/anisole (90:5:3:2)
- S-t-Bu
S-tert-butyl mercapto
- TFA
trifluoroacetic acid
- Trt
triphenylmethyl. Amino acid symbols denote thel-configuration, unless indicated otherwise 相似文献
7.
8.
Zsolt Bozsó Gergely Tóth Richard F. Murphy Sándor Lovas 《Letters in Peptide Science》2000,7(3):157-163
Coupling of amino acids to 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (Tic) and 1,2,3,4-tetrahydro-7-hydroxyisoquinoline-3-carboxylic acid (HOTic) is difficult.In model experiments, use of 1-hydroxy-7-azabenzotriazole(HOAt) in combination with either N,N-diisopropylcarbodiimide (DIC) or O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium (HATU) for activation waseffective in solving coupling difficulties. Based on thisfinding, HOTic was then incorporated into the 20–31 fragmentof human epidermal growth factor (hEGF).[Abu20,31,HOTic22]hEGF(20–31)-NH2was shown to be a `difficult sequence', but replacement of the Tyr at position 29 with HOTic facilitates the complete dodecapeptide synthesis. 相似文献
9.
Leif Christensen Richard Fitzpatrick Brian Gildea Kenneth H. Petersen Henrik F. Hansen Troels Koch Michael Egholm Ole Buchardt Peter E. Nielsen James Coull Rolf H. Berg 《Journal of peptide science》1995,1(3):175-183
Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by O-(benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate in combination with in situ neutralization of the resin allowed efficient coupling of all four Boc-protected PNA monomers within 30 min. HPLC analysis of the crude product obtained from a fully automated synthesis of the model PNA oligomer H-CGGACTAAGTCCATTGC-Gly-NH2, indicated an average yield per synthetic cycle of 97.1%. N1-benzyloxycarbonyl-N63-methylimidazole triflate substantially outperformed acetic anhydride as a capping reagent. The resin-bound PNAs were successfully cleaved by the ‘low–high’ trifluoromethanesulphonic acid procedure. 相似文献
10.
Synthesis of Imidazol-2-yl Amino Acids by Using Cells from Alkane-Oxidizing Bacteria 总被引:1,自引:1,他引:0 下载免费PDF全文
Sixty-one strains of alkane-oxidizing bacteria were tested for their ability to oxidize N-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide to imidazol-2-yl amino acids applicable for pharmaceutical purposes. After growth with n-alkane, 15 strains formed different imidazol-2-yl amino acids identified by chemical structure analysis (mass and nuclear magnetic resonance spectrometry). High yields of imidazol-2-yl amino acids were produced by the strains Gordonia rubropertincta SBUG 105, Gordonia terrae SBUG 253, Nocardia asteroides SBUG 175, Rhodococcus erythropolis SBUG 251, and Rhodococcus erythropolis SBUG 254. Biotransformation occurred via oxidation of the alkyl side chain and produced 1-acetylamino-4-phenylimidazol-2-yl-6-aminohexanoic acid and the butanoic acid derivative. In addition, the acetylamino group of these products and of the substrate was transformed to an amino group. The product pattern as well as the transformation pathway of N-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide differed in the various strains used. 相似文献
11.
Nikhil Sharma Namita Srivastava Ashutosh Kaushal Bhanuranjan Das Aditi Vashistha Dr. Lokender Kumar Dr. Rajnish Kumar Dr. Ashok Kumar Yadav 《化学与生物多样性》2023,20(9):e202300647
The development of bacterial resistance to chemical therapy poses a severe danger to efficacy of treating bacterial infections. One of the key factors for resistance to antimicrobial medications is growth of bacteria in biofilm. Quorum sensing (QS) inhibition was created as an alternative treatment by developing novel anti-biofilm medicines. Cell-cell communication is impeded by QS inhibition, which targets QS signaling pathway. The goal of this work is to develop newer drugs that are effective against Pseudomonas aeruginosa by decreasing QS and acting as anti-biofilm agents. In this investigation, N-(benzo[d]thiazol-2-yl)benzamide/N-(thiazol-2-yl)benzamide derivatives 3a-h were designed and synthesized in good yields. Further, molecular docking analyses revealed that binding affinity values were founded −11.2 to −7.6 kcal/mol that were moderate to good. The physicochemical properties of these prepared compounds were investigated through in-silico method. Molecular dynamic simulation was also used to know better understanding of stability of the protein and ligand complex. Comparing N-(benzo[d]thiazol-2-yl)benzamide 3a to salicylic acid (4.40±0.10) that was utilised as standard for quorum sensing inhibitor, the anti-QS action was found greater for N-(benzo[d]thiazol-2-yl)benzamide 3a (4.67±0.45) than salicylic acid (4.40±0.10). Overall, research results suggested that N-(benzo[d]thiazol-2-yl)benzamide/N-(thiazol-2-yl)benzamide derivatives 3a-h may hold to develop new quorum sensing inhibitors. 相似文献
12.
Two new chiral monochloro-s-triazines (MCT) were synthesized [viz N-(4-chloro-6-piperidinyl-[1,3,5]-triazine-2-yl)-l-leucine amide and N-(4-chloro-6-piperidinyl-[1,3,5]-triazine-2-yl)-l-leucine) (CDR 1 and 2, respectively)] by the nucleophilic displacement of chlorine atoms in s-triazine moiety. One of the Cl atoms was replaced with piperidine, and the second Cl atom in the 6-piperidinyl derivative
was replaced with amino acid amide (viz l-Leu–NH2) and amino acid (l-Leu). These reagents were characterized and used as CDRs for chiral separation of protein and non-protein amino acids, and
were separated on a reversed-phase C18 column. The reaction conditions were optimized for the synthesis of diastereomers using one MCT reagent. The separation method
was validated for limit of detection, linearity, accuracy, precision, and recovery. 相似文献
13.
Bozsó Zsolt Tóth Gergely Murphy Richard F. Lovas Sándor 《International journal of peptide research and therapeutics》2000,7(3):157-163
Summary Coupling of amino acids to 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (Tic) and 1,2,3,4-tetrahydro-7-hydroxyisoquinoline-3-carboxylic
acid (HOTic) is difficult. In model experiments, use of 1-hydroxy-7-azabenzotriazole (HOAt) in combination with eitherN,N-diisopropylcarbodiimide (DIC) orO-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium (HATU) for activation was effective in solving coupling difficulties.
Based on this finding, HOTic was then incorporated into the 20–31 fragment of human epidermal growth factor (hEGF). [Abu20, 31,HOTic22]hEGF(20–31)-NH2 was shown to be a ‘difficult sequence’, but replacement of the Tyr at position 29 with HOTic facilitates the complete dodecapeptide
synthesis. 相似文献
14.
Summary An efficient and generally applicable method for the synthesis of head-to-tail cyclic peptides with HBTU (2-(1H-benzotriazol-1-yl)-1,1,3, 3-tetramethyluronium hexafluorophosphate) has been developed. The utility of this approach was exemplified with the multigram preparation of a potent endothelin receptor-selective (ETA) antagonist BQ-123 (cyclo[-d-Trp-d-Asp-Pro-d-Val-Leu-]). This methodology can be readily applied to the small-and largescale synthesis of other head-to-tail cyclic peptides. 相似文献
15.
A. Meyer J. Schmidt D. Gross E. Jensen A. Rudolph S. Vorkefeld G. Sembdner 《Journal of Plant Growth Regulation》1991,10(1):17-25
The biotransformation of [2-14C](±)9, 10-dihydrojasmonic acid (DJA) was studied in excised shoots of 6-day-old barley seedlings after 72 h. From the ethyl acetate extract, some minor metabolites were isolated and purified by DEAE-Sephadex A-25 chromatography, thin-layer chromatography (TLC), C18-cartridges, and high-performance liquid chromatography (HPLC). The structural identification of these metabolites was performed by gas chromatography-mass spectrometry (GC-MS), circular dichroism (CD), and amino acid analysis, and the following amino acid conjugates were found:N-[(–)9,10-dihydrojasmonoyl]valine,N-[(–)9,10-dihydrojasmonoyl]isoleucine,N-[9,10-dihydrojasmonoyl]leucine,N-[11-hydroxy-9,10-dihydrojasmonoyl]valine,N-[11-hydroxy-9,10-dihydrojasmonoyl]isoleucine,N-[12-hydroxy-9,10-dihydrojasmonoyl]isoleucine; and the cucurbic acid-related compoundsN-{[3-hydroxy-2(4-hydroxypentyl)-cyclopent-1-yl]-acetyl}isoleucine andN-{[3-hydroxy-2(5-hydroxypentyl)-cyclopent-1-yl]-acetyl}isoleucine. The results suggest conjugation with isoleucine and valine, as well as preferential hydroxylation at position C-11 or hydrogenation at position C-6, as being important steps in the metabolism of (±)DJA in barley shoots. 相似文献
16.
《Chemical Speciation and Bioavailability》2013,25(2):127-134
AbstractA simple and rapid procedure is developed for the quantitative flotation of mercury(II) from aqueous solutions. Thiosemicarbazide derivatives such as: 1-(amino-N-phenylmethanethio)-4-(pyridine-2-yl)thio-semicarbazide (H2PPS), N-phenyl-2-(pyridine-2-ylcarbamothioyl) hydrazinecarboxamide (H2PBO), 1-(amino(thioformyl)-N-phenylform)-4-(pyridine-2-yl)thiosemicarbazide (H2APO), and 1-(amino-N-(pyri-dine-3-yl)methanethio)-4-(pyridine-2-yl)thiosemicarbazide (H2PPY) are used as organic chelating agents and oleic acid (HOL) as surfactant. The different parameters affecting the flotation process namely, metal ion, ligands and surfactant concentrations, foreign ions (which are normally present in fresh and saline waters), pH and temperature are examined. Nearly 100% of mercury ions are floated at a metal:ligand ratio of 1: 4, pH ~ 4 and at room temperature (~25°C). The procedure was successfully applied to recover Hg2+ ions spiked into some water samples. A flotation mechanism is suggested based on some physical and chemical studies on the solid complexes isolated from aqueous and floated layers. 相似文献
17.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):733-743
AbstractTo explore the pharmacological and structure–activity relationship of a series of N-substituted-(4-oxo-2-substituted-phenylquinazolin-3-(4H)-yl), substituted benzene sulfonamide derivatives (1–25) were synthesized from substituted anthranilic acids derived amino quinazolines and substituted benzene sulphonamides. All the synthesized compounds were evaluated for their diuretic (by Lipschitz et al. method), antihypertensive activity by non-invasive blood pressure (NIBP) using the tail-cuff method and anti-diabetic potential in rats. Six compounds showing significantly excellent activity were compared with metolazone, prazosin and diazoxide as standards. Compound N-[7-chloro-2-(4-methoxyphenyl)-4-oxoquinazolin-3(4H)-yl]-4 nitrobenzenesulfonamide (20) exhibited most potent of the series. 相似文献
18.
Yoshitami Ohashi Hiroshi Abe Yukio Ito 《Bioscience, biotechnology, and biochemistry》2013,77(10):2277-2282
Acid hydrolysis of the antibiotic YA-56 X (Zorbamycin) and Y belonging to the phleomycin-bleomycin group was carried out and the following constitutional amino acids were isolated from the hydrolyzate of YA–56 X: β-Amino-β-(4-amino-6-carboxy-5-methylpyrimidine-2-yl)- propionic acid, β-aminoalanine, L-erythro-β-hydroxyhistidine and 3 unidentified amino acids. Though the former 3 amino acids were known to be constituents of phleomycins and bleomycins, the latter three were not found in phleomycins and bleomycins. YA–56 Y gave one more unidentified amino acid.Furthermore, isolation of β-alanine and 2-acetylthiazole-4-carboxylic acid from the hydrolyzate indicated the presence of 2-(2-(2-aminoethyl)-Δ2-thiazoline-4-yl)-thiazole-4-carboxylic acid in YA–56 X and Y as in phleomycins. 相似文献
19.
Dimitris Panagiotopoulos John M. Matsoukas Kostas Alexopoulos Anna Zebeki Thomas Mavromoustakos Mahmoud Saifeddine Morley D. Hollenberg 《Letters in Peptide Science》1996,3(4):233-240
Summary Cyclic analogues of the active thrombin receptor peptide SFLLR (TRP42–46) containingd-Phe and/ord-Arg have been prepared by the solid-phase method, purified by reversed-phase HPLC and bioassayed in a rat smooth muscle contractile assay. Cyclization was achieved by forming an amide linkage between the-NH2 and-COOH groups of the two leucine residues located at the N- and C-terminal positions of the linear protected precursor H2N-Leu-Arg(Pmc)-Y-Phe-Leu-OH (Y=Gly,Acp) using 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluoroborate borate (HBTU) or 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU) as coupling reagents andN,N-diisopropylethylamine (DIPEA) in high dilution. Their structure was confirmed by fast by fast atom bombardment mass spectrometry and NMR methods. The cyclic peptides c-fLLrG, c-fLLRG, c-FLLrG and c-fLLrAcp, c-FLLrAcp so synthesized were assessed for their contractile activity in a rat gastric longitudinal muscle bioassay system which has been used previously to evaluate the biological activities of linear thrombin-receptor-derived polypeptides such as SFLLR (P5) and SFLLR-NH2 (P5-NH2). 相似文献
20.
Carlos García-Echeverría 《Letters in Peptide Science》1996,2(6):369-373
Summary In order to minimise the formation of the pyrophosphate derivative of the target peptide when side-chain-unprotected phopshotyrosine is used in solid-phase peptide synthesis, this building block can be incorporated using benzotriazolyloxy-tris-(dimethylamino)phosphonium hexafluorophosphate/1-hydroxybenzotriazole/N-methylmorpholine (1:1:2.3) in the presence of a chaotropic salt (0.4 M LiCl in N-methyl-2-pyrrolidinone).Abbreviations BOP
benzotriazolyloxy-tris-(dimethylamino)phosphonium hexafluorophosphate
- DIEA
diisopropylethylamine
- Fmoc
9-fluorenylmethoxycarbony
- HATU
N-[(dimethylamino)1H-1,2,3-triazolo[4,5-b]pyridin-1-ylmethylene]-N-methylmethan-aminium hexafluorophosphate N-oxide
- HOBt
1-hydroxybenzotriazole
- HPLC
high-performance liquid chromatography
- MALDI-TOF
matrix-assisted laser-desorption ionization time-of-flight mass spectrometry
- NMM
N-methylmorpholine
- NMP
N-methyl-2-pyrrolidinone
- Pmc
2,2,5,7,8-pentamethyl-chroman-6-sulfonyl
- ®
solid support
- TFA
trifluoroacetic acid
- TPTU
2-(2-pyridon-1-yl)-1,1,3,3-tetramethyluroniumfluoroborate. Abbreviations used for amino acids follow the recommendations of the IUPAC-IUB Commission of Biochemical Nomenclature [Eur. J. Biochem., 138 (1984) 9] 相似文献