白介素15

白介素１５王东勇,黄建生,郭明秋（第一军医大学免疫学教研室,广州５１０５１５）关键词白介素１５白介素１５（ＩＬ－１５）是新近发现的一种细胞因子,来源于多种细胞和组织,其ｃＤＮＡ已被分离并在哺乳动物细胞和酵母中表达。现已证明,ＩＬ－１５可以促进淋巴细胞...     

Duplication 15q22»15qter and its phenotypic expression

Summary Two infants with dysmorphism and aortic stenosis were trisomic for the distal part of 15q. Similar to seven other published cases, the facial dysmorphism was characteristic: prominent nose, narrow palpebral fissures, slight anti-mongoloïd slant, low-set ears, long upper lip, and a pronounced philtrum. Laboratory studies failed to demonstrate a gene-dose effect for the enzymes coded by chromosome 15 (PK 3 and MPI) and chromosome 21 (SOD 1).     

白细胞介素—15研究进展

ＩＬ－１５是近年来发现的一种较为重要的免疫因子。它与ＩＬ－２共享ＩＬ－２Ｒβ,γ亚基,因此二者有许多相似的功能。如激活Ｔ细胞和Ｂ细胞,诱导ＮＫ细胞的ＬＡＫ活性等。本文对ＩＬ－１５的基因及蛋白结构,对免疫细胞的作用,信号转导途径,与ＩＬ－２的关系以及在一些疾病中的作用进行了综述。     

白细胞介素－15研究进展

白细胞介素一15是一种最近被正式承认的新细胞因子,该因子cDNA结构和功能及分子结构已阐明,其生物学活性和空间构象与IL-2非常相似但又有差异,IL-15受体至少包括IL-2受体β和γ链亚单位,是否尚有其他未知成分参与IL-15受体的组成和其详细的信号传递途径以及其他未知生物学活性如何尚有待深入研究.     

第15卷关键词索引

第１５卷关键词索引白头鸭１５（３）：１９白蚁１５（２）：８５斑翅山鹑１５（４）：３７半翅目１５（３）：１斑点杂交试验１５（１）：８５斑羚１５（４）：４９斑腿蝗科１５（１）：２９蝙蝠蛾科１５（３）：５比较种群生态学１５（２）：３２Ｂ细胞１５（３）：８１...     

质粒pMM15的构建

以pLJ3和pKO-6质粒DNA为材料,经体外重组构建了质粒pMM15。该质粒带有一个lac UV5启动子片段和一个半乳糖激酶基因。二者之间有单一的EcoRI位点。此外,还有一个Amp~1药物抗性标记。分子量约为3.8kb。该质粒在受体细胞内能够产生有活性的半乳糖激酶。在EcoRI位点插入外源DNA后,则半乳糖激酶基因表达受阻。因而在用于研究外源基因的表达及无性繁殖试验中,可通过遗传互补试验,在麦康凯半乳糖指示剂平板上,根据菌落颜色的改变来检出重组转化子。     

Characterization and Favorable in Vivo Properties of Heterodimeric Soluble IL-15·IL-15Rα Cytokine Compared to IL-15 Monomer

Interleukin-15 (IL-15), a 114-amino acid cytokine related to IL-2, regulates immune homeostasis and the fate of many lymphocyte subsets. We reported that, in the blood of mice and humans, IL-15 is present as a heterodimer associated with soluble IL-15 receptor α (sIL-15Rα). Here, we show efficient production of this noncovalently linked but stable heterodimer in clonal human HEK293 cells and release of the processed IL-15·sIL-15Rα heterodimer in the medium. Purification of the IL-15 and sIL-15Rα polypeptides allowed identification of the proteolytic cleavage site of IL-15Rα and characterization of multiple glycosylation sites. Administration of the IL-15·sIL-15Rα heterodimer reconstituted from purified subunits resulted in sustained plasma IL-15 levels and in robust expansion of NK and T cells in mice, demonstrating pharmacokinetics and in vivo bioactivity superior to single chain IL-15. These identified properties of heterodimeric IL-15 provide a strong rationale for the evaluation of this molecule for clinical applications.     

植物学报 第15卷 总目录

第1期(1973年6月)小麦(T万‘c1’二,lga、)花粉植株的诱导及其形态发生过程的研究朱至清王敬驹孙敬三钱南芬尹光初徐振(1)在人工培养条件下君子兰(Cli衍a二obilis)雄配子体发育过程中营养核与生殖细胞的动态—显微缩时电影记录与一般细胞学观察····.·.·....................……植物抗寒性的细胞学研究一一小麦进人寒冬时期细胞亚显微结构的变化··~····~··…简令成费草伽icoti即。tabac“水)和辣椒(c aPsic“、阴洲“二)花药离体培养的研究········……······……唐佩华(12)荆玉祥张宝田(22)···一····…     

遗传学报第15卷总目录

第1期苏联球茎大麦有利基因引人普通小麦的研究·········”·········································…… ···········································一汪丽泉、朱汉如、戎均康、梁竹青、郑毅仁、管启良(1.)起源于组织培养的釉稻雄性不育体细胞无性系变异···……凌定厚、马镇荣、陈婉瑛、陈梅芳(,)约束选择在小麦育种中的应用研究····”··········································…     

疱疹病毒UL15基因的研究进展

UL15基因在疱疹病毒科中高度保守,编码的pUL15在病毒DNA包装过程中具有重要作用。本文从UL15基因的序列特点,编码蛋白氨基酸的特点,基本功能以及与pUL6、pUL28、pUL33的相互作用等方面进行概述,以期为研究pUL15在疱疹病毒感染和复制过程中的作用提供一定参考。     

动物学报第15卷总目录

第l期阴道毛滴虫的研究v工.几种培养基对牛胎毛滴虫(丁ric加、口二,foet“,) 培养的比较贰脸··】·1·········‘···································……奥硕显、叶英(1)蝶水母变态的研究,····,·······································,···,········,·····……丘书院(1。)红螺(口、,‘“厉“加p。后)的晶柱··········,··,·····...················4·····一榭麟阴(片)同域分布的儿种斧…     

白介素-15与脂质代谢

近些年IL-15对脂肪组织代谢的影响备受关注。IL-15可以抑制机体LPL合成,降低甘油三酯的水平,从而减少脂肪的蓄积。进一步研究发现,IL-15可以通过激活p42/p44 MAPK信号通路抑制脂肪细胞增殖活性,降低STAT5a蛋白表达抑制脂肪细胞分化,同时激活SAPK/JNK通路促使细胞凋亡。IL-15还可以上调Ca2+磷酸酶的活性,阻断脂肪细胞分化。本文将IL-15对脂代谢的影响进行简要概述。     

1％三唑酮+15％乙蒜素WP和15％多菌灵+15％福美双WP对变叶木插穗的影响

以琴叶变叶木(Codiaeum varie gatum var.pictum)为材料,用1%三唑酮 15%乙蒜素可湿性粉剂(三乙)和15%多菌灵 15%福美双可湿性粉剂(多福)分别配制成不同浓度的系列溶液,对插穗浸泡处理3h后进行扦插试验,研究这两种混合杀菌剂对插穗生根的影响。结果表明,三乙和多福处理的最佳插穗平均生根数、平均根长、平均根直径、成活率分别高出对照26.2、15.4mm、0.25mm、60%和9.6、7.4mm、0.36mm、46.67%,与对照比较差异达极显著水平(P!0.01)。三乙和多福浸泡3h的最佳浓度分别为1.819gL-1和1.894gL-1,三乙浸泡优于多福(P!0.01)。     

Different dynamics of IL-15R activation following IL-15 cis- or trans-presentation

Interleukin (IL)-15 is a cytokine critical for the homeostasis and the function of NK cells, NK-T cells, and memory CD8+ T cells. IL-15 signals are delivered through the IL-15Rβ and the common γ (γ(c)) receptor chains. The third receptor chain, IL-15Rα, confers specificity and high affinity for the cytokine. While IL-15 can activate with high affinity the trimeric receptor expressed by a target cell (cis-presentation), IL-15Rα is also known to trans-present IL-15 with high affinity to target cells expressing the IL-15Rβ/γ(c) complex. In order to compare the IL-15 cis- and trans-presentation processes, and using a T cell line expressing both IL-15Rα/β/γ(c) and IL-15Rβ/γ(c), we analyzed cell surface receptor chain down-modulation, cytokine internalization and signaling responses induced either with IL-15 (cis-presentation) or with RLI, a protein resulting from fusion between IL-15 and an extended IL-15Rα sushi domain, that mimics trans-presentation. Whereas IL-15 bound with high affinity to IL-15Rα/β/γ(c), RLI bound with a similar high affinity to IL-15Rβ/γ(c). The kinetics of cell surface IL-15R down-modulation were slower following RLI treatment than after IL-15 treatment, as were the kinetics of RLI internalization, which was slower than that of IL-15. IL-15 and RLI dose-dependently induced the activation of similar signaling pathways. However, the kinetics and duration of these activations were markedly different, RLI-induced signaling, being slower, but more prolonged than that induced by IL-15, although the final proliferative responses at 48?h were similar. These findings collectively indicate that IL-15 cis- and trans-presentation mechanisms lead to different dynamics of receptor activation and signal transduction, with cis-presentation inducing fast and transient responses, and trans-presentation inducing slower, more persistent ones. They provide clues for a better understanding of how IL-15 action is controlled, and how it plays a key role in the coordination between innate and adaptative immunity.     

15年的鄱阳湖记忆

初识鄱阳湖是20世纪80年代末的一个冬天,我第一次去鄱阳湖国家级自然保护区考察学习。一行四人乘坐吉普车,从南昌市出发,由时任保护区管理处主持工作的刘副处长带领,经永修县的三角乡到达保护区的大湖池。记得当时已是下午5点左右,放眼望去,越过高高的大堤便是一望无垠的湖水、南荻     

十二指肠损伤15例诊治体会

目的：提高对十二指肠损伤的诊治水平,减少漏诊,降低病死率.方法：回顾性总结分析十二指肠损伤15例临床资料.结果：15例分别行单纯修补、端端吻合、十二指肠-空肠-Roux-en-Y吻合、憩室化手术,13例痊愈.2例死亡,病死率13.3%.结论：单纯十二指肠损伤修补加十二指肠减压对大多数十二指肠损伤是最简单有效的手术方法.早期诊断、合理处理、防止术中漏诊才能降低病死率.     

白细胞介素—15及其受体

白细胞介素－１５是一种新发现的细胞因子,其生物学性质与ＩＬ－２相似,ＩＬ－１５的受体由ＩＬ－１５Ｒａ,ＩＬ－２Ｒβ,ＩＬ－２Ｒγ组成,其中ＩＬ－２Ｒγ是参与ＩＬ－１５信号传递过程的必要成分。ＩＬ－１５和ＩＬ－２的体内表达及受体分布存在差异预示着ＩＬ－１５在体内具有独特的生物学作用。