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1.
Adam Ahanchédé José E. F. Alfaya L. W. Andersen Didier Azam Ma. Anita M. Bautista Anne‐Laure Besnard Gregorio Bigatti Anthony Bouétard Marie‐Agnès Coutellec Eben‐Ezer B. K. Ewédjè Reiko Fuseya Ricardo GarcÍa‐Jiménez M. Haratian Olivier J. Hardy L.‐E. Holm Casey W. Hoy Eriko Koshimizu V. Loeschcke Violeta López‐Márquez Carlos A. Machado Annie Machordom C. Marchi Andrew P. Michel Claire Micheneau Omprakash Mittapalli Takahiro Nagai Nobuaki Okamoto Ying Pan F. Panitz N. Safaie Takashi Sakamoto B. Sharifnabi En‐Wei Tian Hui Yu 《Molecular ecology resources》2013,13(1):158-159
This article documents the addition of 83 microsatellite marker loci and 96 pairs of single‐nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bembidion lampros, Inimicus japonicus, Lymnaea stagnalis, Panopea abbreviata, Pentadesma butyracea, Sycoscapter hirticola and Thanatephorus cucumeris (anamorph: Rhizoctonia solani). These loci were cross‐tested on the following species: Pentadesma grandifolia and Pentadesma reyndersii. This article also documents the addition of 96 sequencing primer pairs and 88 allele‐specific primers or probes for Plutella xylostella. 相似文献
2.
Molecular Ecology Resources Primer Development Consortium Arias MC Arnoux E Bell JJ Bernadou A Bino G Blatrix R Bourguet D Carrea C Clamens AL Cunha HA d'Alençon E Ding Y Djieto-Lordon C Dubois MP Dumas P Eraud C Faivre B Francisco FO Françoso E Garcia M Gardner JP Garnier S Gimenez S Gold JR Harris DJ He G Hellemans B Hollenbeck CM Jing S Kergoat GJ Liu B McDowell JR McKey D Miller TL Newton E Pagenkopp Lohan KM Papetti C Paterson I Peccoud J Peng X Piatscheck F Ponsard S Reece KS Reisser CM 《Molecular ecology resources》2012,12(3):570-572
This article documents the addition of 473 microsatellite marker loci and 71 pairs of single‐nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Barteria fistulosa, Bombus morio, Galaxias platei, Hematodinium perezi, Macrocentrus cingulum Brischke (a.k.a. M. abdominalis Fab., M. grandii Goidanich or M. gifuensis Ashmead), Micropogonias furnieri, Nerita melanotragus, Nilaparvata lugens Stål, Sciaenops ocellatus, Scomber scombrus, Spodoptera frugiperda and Turdus lherminieri. These loci were cross‐tested on the following species: Barteria dewevrei, Barteria nigritana, Barteria solida, Cynoscion acoupa, Cynoscion jamaicensis, Cynoscion leiarchus, Cynoscion nebulosus, Cynoscion striatus, Cynoscion virescens, Macrodon ancylodon, Menticirrhus americanus, Nilaparvata muiri and Umbrina canosai. This article also documents the addition of 116 sequencing primer pairs for Dicentrarchus labrax. 相似文献
3.
Molecular Ecology Resources Primer Development Consortium Abreu AG Albaina A Alpermann TJ Apkenas VE Bankhead-Dronnet S Bergek S Berumen ML Cho CH Clobert J Coulon A DE Feraudy D Estonba A Hankeln T Hochkirch A Hsu TW Huang TJ Irigoien X Iriondo M Kay KM Kinitz T Kothera L LE Hénanff M Lieutier F Lourdais O Macrini CM Manzano C Martin C Morris VR Nanninga G Pardo MA Plieske J Pointeau S Prestegaard T Quack M Richard M Savage HM Schwarcz KD Shade J Simms EL Solferini VN Stevens VM Veith M Wen MJ 《Molecular ecology resources》2012,12(2):374-376
This article documents the addition of 139 microsatellite marker loci and 90 pairs of single‐nucleotide polymorphism sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Aglaoctenus lagotis, Costus pulverulentus, Costus scaber, Culex pipiens, Dascyllus marginatus, Lupinus nanus Benth, Phloeomyzus passerini, Podarcis muralis, Rhododendron rubropilosum Hayata var. taiwanalpinum and Zoarces viviparus. These loci were cross‐tested on the following species: Culex quinquefasciatus, Rhododendron pseudochrysanthum Hay. ssp. morii (Hay.) Yamazaki and R. pseudochrysanthum Hayata. This article also documents the addition of 48 sequencing primer pairs and 90 allele‐specific primers for Engraulis encrasicolus. 相似文献
4.
M. C. Arias Christiane Atteke S. C. Augusto J. Bailey Pilar Bazaga Luciano B. Beheregaray Laure Benoit Rumsaïs Blatrix Céline Born R. M. Brito Hai‐kui Chen Sara Covarrubias Clara de Vega Champlain Djiéto‐Lordon Marie‐Pierre Dubois F. O. Francisco Cristina García P. H. P. Gonçalves Clementina González Carla Gutiérrez‐Rodríguez Michael P. Hammer Carlos M. Herrera H. Itoh S. Kamimura H. Karaoglu S. Kojima Shou‐Li Li Hannah J. Ling Pável F. Matos‐Maraví Doyle McKey Judicaël Mezui‐M'Eko Juan Francisco Ornelas R. F. Park María I. Pozo Satu Ramula Cristina Rigueiro Jonathan Sandoval‐Castillo L. R. Santiago Miyuki M. Seino Chang‐Bing Song H. Takeshima Anti Vasemägi C. R. Wellings Ji Yan Du Yu‐Zhou Chang‐Rong Zhang Tian‐Yun Zhang 《Molecular ecology resources》2013,13(4):760-762
This article documents the addition of 142 microsatellite marker loci to the Molecular Ecology Resources database. Loci were developed for the following species: Agriophyllum squarrosum, Amazilia cyanocephala, Batillaria attramentaria, Fungal strain CTeY1 (Ascomycota), Gadopsis marmoratus, Juniperus phoenicea subsp. turbinata, Liriomyza sativae, Lupinus polyphyllus, Metschnikowia reukaufii, Puccinia striiformis and Xylocopa grisescens. These loci were cross‐tested on the following species: Amazilia beryllina, Amazilia candida, Amazilia rutila, Amazilia tzacatl, Amazilia violiceps, Amazilia yucatanensis, Campylopterus curvipennis, Cynanthus sordidus, Hylocharis leucotis, Juniperus brevifolia, Juniperus cedrus, Juniperus osteosperma, Juniperus oxycedrus, Juniperus thurifera, Liriomyza bryoniae, Liriomyza chinensis, Liriomyza huidobrensis and Liriomyza trifolii. 相似文献
5.
V. Mohindra R. K. Singh M. Palanichamy A. G. Ponniah K. K. Lal 《Zeitschrift fur angewandte Ichthyologie》2007,23(1):104-109
Genetic variability in three clariid species, Clarias batrachus, C. gariepinus and C. macrocephalus, were investigated by allozyme electrophoresis and mitochondrial DNA (ND 5/6) RFLP markers. The catfish species C. batrachus and C. macrocephalus are native to the Indian subcontinent and Southeast Asia, respectively, while the African catfish C. gariepinus was introduced to Asian countries for culture. Sixteen gene loci from 12 enzyme systems were analyzed. Fixed allelic differences were evident between pairs of species at least at four loci. In mtDNA RFLP analysis, eight composite haplotypes were observed and each species was characterized by a set of haplotypes. The UPGMA phylogenetic tree revealed three distinct clusters: C. batrachus; C. gariepinus (India and Thailand); and C. macrocephalus. 相似文献
6.
Mitochondrial DNA cytochrome b (cyt b) sequence variation among the clariid fishes of Lake Victoria and the Malagarasi wetland Tanzania were studied between August 2003 and February 2005. Seven species were sampled and together with 26 cyt b sequences from GenBank were used to reconstruct phylogenetic relationships in the family Clariidae. The study revealed two clades: one consisting of the big‐head species, Clarias gariepinus and Heterobranchus longifilis, and the other of small‐sized species, Clarias werneri, Clarias alluaudi, Clarias liocephalus and Clariallabes petricola. The study further revealed that the genus Clarias is paraphyletic and that H. longifilis clusters deeply inside the Clarias group. Although H. longifilis is thought to be the oldest in evolutionary age in Tanzania, it is not close to the ancestor of the Clariidae family. The results also showed that C. werneri and C. alluaudi are genetically distinct from each other. 相似文献
7.
Alex C. C. Wilson Blandine Massonnet Jean‐Christophe Simon Nathalie Prunier‐Leterme Lotfali Dolatti Kate S. Llewellyn Christian C. Figueroa Claudio C. Ramirez Roger L. Blackman Arnaud Estoup Paul Sunnucks 《Molecular ecology resources》2004,4(1):104-109
Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross‐species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross‐species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids. 相似文献
8.
Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2012 - 31 May 2012
Molecular Ecology Resources Primer Development Consortium P Abelló W Ai C Altmann G Bernardi O Bonato KM Burchhardt X Chen Z Chen D Cížková C Clouet MA Cubeta V Garcia-Merchan N Gauthier S Gibson K Halačka F Hamdi T Hankeln A Hochkirch T Hrbek AM Jackson C Lin SM Lin E Macpherson J Macrander E Marešová J Mendel M Nowak G Orti F Palero I Papoušek M Pascual T Schmitt BX Semmens JC Streito EW Tian SP Tseng M Veith L Vetešník HY Wang J Weyer S Willis H Yu Z Zhou 《Molecular ecology resources》2012,12(5):972-974
This article documents the addition of 123 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brenthis ino, Cichla orinocensis, Cichla temensis, Epinephelus striatus, Gobio gobio, Liocarcinus depurator, Macrolophus pygmaeus, Monilinia vaccinii-corymbosi, Pelochelys cantorii, Philotrypesis josephi, Romanogobio vladykovi, Takydromus luyeanus and Takydromus viridipunctatus. These loci were cross-tested on the following species: Cichla intermedia, Cichla ocellaris, Cichla pinima, Epinephelus acanthistius, Gobio carpathicus, Gobio obtusirostris, Gobio sp. 1, Gobio volgensis, Macrolophus costalis, Macrolophus melanotoma, Macrolophus pygmaeus, Romanogobio albipinnatus, Romanogobio banaticus, Romanogobio belingi, Romanogobio kesslerii, Romanogobio parvus, Romanogobio pentatrichus, Romanogobio uranoscopus, Takydromus formosanus, Takydromus hsuehshanesis and Takydromus stejnegeri. 相似文献
9.
Mohindra V Singh A Barman AS Tripathi R Sood N Lal KK 《Molecular biology reports》2012,39(5):5921-5931
Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources
in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated
with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained,
out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs
containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function
and 1 for hypothetical protein. Nine ESTs with SSRs and/or SNPs identified in this study were reported to be associated with
diseases in human and animals. These identified loci can be developed into markers in C. batrachus, which can be useful in linkage mapping, comparative genomics studies and for its genetic improvement programmes. 相似文献
10.
Isolation and characterization of microsatellites in the Asian walking catfish Clarias macrocephalus
Microsatellite loci were characterized in the walking catfish, Clarias macrocephalus, random clones from a small genomic library using a (GT)15 probe. Primers for DNA amplifications using polymerase chain reaction (PCR) were designed and synthesized for 23 loci. Twelve loci were polymorphic, with the number of alleles ranging from two to 13 alleles per locus. Developed microsatellite primers should prove useful for population studies and genetic mapping of the walking catfish. 相似文献
11.
TONIA S. SCHWARTZ DANIEL A. WARNER LUCIANO B. BEHEREGARAY MATS OLSSON 《Molecular ecology resources》2007,7(3):528-531
We characterize 15 microsatellite loci from two microsatellite libraries developed from the Australian agamid lizards Amphibolurus muricatus and Ctenophorus pictus. All loci were tested for amplification in four other agamids: Ctenophorus fordi, Ctenophorus decresii, Chlamydosaurus kingii, and Physignathus lesueurii. These loci were highly polymorphic within and across species, with nine to 12 loci amplifying for each species tested. 相似文献
12.
We isolated 18 novel microsatellite loci from the walking catfish (Clarias batrachus), and examined their cross‐amplification in seven additional catfish species from three families. Sixteen of the 18 microsatellites were polymorphic in the source species (allele number: 2–10/locus and expected heterozygosity: 0.30–0.87). Moreover, nine of these 18 primer pairs cross‐amplified specific and polymorphic products from the genome of at least six of the seven other catfish species tested. However, the success rate of cross‐species amplification varied from locus to locus, indicating that cross‐species amplification of microsatellites is locus‐dependent. 相似文献
13.
Yu. F. Kartavtsev V. V. Sviridov N. Hanzawa T. Sasaki 《Russian Journal of Genetics》2002,38(11):1285-1297
Based on a biochemical–genetic approach, heterozygosity and divergence of structural genes at 30 enzyme loci were analyzed in six dace species. In addition, intra- and interspecific divergence of gene expression was analyzed based on a sample of 12 to 15 loci. Mean heterozygosities per individual varied as follows: Tribolodon species, H
obs = 0.007 ± 0.007 and H
exp = 0.007 ± 0.007; T. ezoe, H
obs = 0.045 ± 0.016 and H
exp = 0.067 ± 0.029. Several variants of genetic distances were estimated. Standard Nei's distances (D
N) varied from 0.145 to 0.284 in four dace species studied. As related to Tribolodon dace species, the following genetic distances were obtained for two members of other genera:Pseudaspius leptocephalus, D
N = 0.269; Leuciscus waleckii, D
N = 0.769. Based on the distance matrices, different clustering algorithms were realized. The main feature shared by different dendrograms was a separate position of the cluster joining Far-Eastern dace species, to whichP. leptocephalus and L. waleckii are successively added. Among the species studied, the proportion of loci similar by expression (E) varied from 87 to 100%. The greatest difference was found between landlocked and landlocked ecotypes of T. hakonensis, E = 67%. The following conclusions can be made: (1) Four studied species of the genus Tribolodon are rather well genetically differentiated. Diagnostic loci are available. (2) A nominal dace species, T. species, should be considered the fourth unambigous species of this genus, which is confirmed by its recent zoological acceptance of this species. (3) The origin and divergence of dace species belonging to the genus Tribolodon are relatively late (1 to 3 Myr ago) historical events. (4) Taxonomically, the genus Tribolodon belong to the tribe Pseudaspinini together with P. leptocephalus, which is confirmed by genetic data. (5) Data on heterozygosity and the divergence of structural and regulatory elements of genome, along with the proposed scheme of speciation types, suggest the following speciation modes for the species studied: for four species, adaptive divergence and for two species, genetic transformation. 相似文献
14.
Molecular Ecology Resources Primer Development Consortium Andris M Arias MC Barthel BL Bluhm BH Bried J Canal D Chen XM Cheng P Chiappero MB Coelho MM Collins AB Dash M Davis MC Duarte M Dubois MP Françoso E Galmes MA Gopal K Jarne P Kalbe M Karczmarski L Kim H Martella MB McBride RS Negri V Negro JJ Newell AD Piedade AF Puchulutegui C Raggi L Samonte IE Sarasola JH See DR Seyoum S Silva MC Solaro C Tolley KA Tringali MD Vasemägi A Xu LS Zanón-Martínez JI 《Molecular ecology resources》2012,12(4):779-781
This article documents the addition of 171 microsatellite marker loci and 27 pairs of single nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bombus pauloensis, Cephalorhynchus heavisidii, Cercospora sojina, Harpyhaliaetus coronatus, Hordeum vulgare, Lachnolaimus maximus, Oceanodroma monteiroi, Puccinia striiformis f. sp. tritici, Rhea americana, Salmo salar, Salmo trutta, Schistocephalus solidus, Sousa plumbea and Tursiops aduncus. These loci were cross-tested on the following species: Aquila heliaca, Bulweria bulwerii, Buteo buteo, Buteo swainsoni, Falco rusticolus, Haliaeetus albicilla, Halobaena caerulea, Hieraaetus fasciatus, Oceanodroma castro, Puccinia graminis f. sp. Tritici, Puccinia triticina, Rhea pennata and Schistocephalus pungitii. This article also documents the addition of 27 sequencing primer pairs for Puffinus baroli and Bulweria bulwerii and cross-testing of these loci in Oceanodroma castro, Pelagodroma marina, Pelecanoides georgicus, Pelecanoides urinatrix, Thalassarche chrysostoma and Thalassarche melanophrys. 相似文献
15.
16.
NATALIE M. SCHREY AARON W. SCHREY EDWARD J. HEIST JOHN D. REEVE 《Molecular ecology resources》2007,7(5):857-859
We developed microsatellite loci for the southern pine beetle (Dendroctonus frontalis). Twelve microsatellite loci were identified. Eight loci were polymorphic and sufficiently variable in 62 individuals (expected heterozygosity ranged from 0.707 to 0.880) to investigate population structure. All loci conformed to HWE except Dfr‐14, which showed heterozygote excess, and no two loci deviated from linkage equilibrium. The loci were tested for cross‐species amplification in four species of Dendroctonus (D. valens, D. terebrans, D. brevicomis, and D. ponderosae). Seven loci were polymorphic in at least one of the species tested. 相似文献
17.
Genomic Resources Development Consortium Stephen R. Keller David M. Nelson Cortney Pylant Scott R. Santos Regina Trott 《Molecular ecology resources》2014,14(2):435-436
This article documents the public availability of (i) genomic sequence data and 43 microsatellite loci for the bat species, Lasiurus borealis and Lasiurus cinereus, and (ii) complete mitochondrial and partial nuclear genomes for two jack species, Caranx ignobilis, Caranx melampygus. 相似文献
18.
Two microsatellite‐enriched libraries [(CAGA)n, (TAGA)n] were constructed using pooled DNA from three cyprinid species native to the Sacramento–San Joaquin Delta of California: Sacramento splittail (Pogonichthys macrolepidotus); Sacramento pikeminnow (Ptychocheilus grandis); and tui chub (Siphateles bicolor). Primers were designed for 105 loci and tested for levels of polymorphism in five cyprinid species found in the Delta: Sacramento splittail, Sacramento pikeminnow, tui chub, hitch (Lavinia exilicauda), and Sacramento blackfish (Orthodon microlepidotus). Fifty‐one loci were polymorphic for at least one species and 31 loci were polymorphic for multiple species. The number of polymorphic loci per species ranged from 16 to 26. 相似文献
19.
The control of the maize weevil, Sitophilus zeamais in brown rice was estimated by using a reduviid bug, Amphibolus venator, and a pteromalid wasp, Theocolax elegans. Results showed that the simultaneous release of both species of natural enemies could enhance biological control compared with the release of either species alone. 相似文献
20.
Marthajane Caldwell Michael S. Gaines Colin R. Hughes 《Molecular ecology resources》2002,2(4):393-395
We developed eight polymorphic microsatellite loci that can be used to elucidate population structure and aid management of bottlenose dolphins, Tursiops truncatus, in the northwest Atlantic. These loci averaged nine alleles, with no evidence for null alleles. Their cross‐species utility was examined in Cephalorhynchus commersonii, Delphinus delphis, Delphinapterus leucas, Eschrictius robustus, Globicephala macrorhynchus, Lagenorhynchus obliquidens, Orcinus orca, Steno bredanensis and Stenella clymene. On average, 75% of the loci were polymorphic in these species (range = 50–88%). Our results indicate that these loci will be useful for elucidating population structure of bottlenose dolphin as well as of other cetacean species. 相似文献