EFFECTS OF CANOPY POSITION AND IRRADIANCE ON THE LEAF PHYSIOLOGY AND MORPHOLOGY OF PENTACLETHRA MACROLOBA (MIMOSACEAE)

Pentaclethra macroloba (Willd.) Kuntze (Mimosaceae) is a dominant late-successional tree species in the Atlantic lowland forests of Costa Rica. Leaves of P. macroloba from three heights in the forest canopy were compared with leaves of seedlings grown in controlled environment chambers under four different irradiance levels. Changes in leaf characteristics along the canopy gradient paralleled changes resulting from the light gradient under controlled conditions. The effect of light or canopy position on light-saturated photosynthesis was small, with maximum photosynthesis increasing from 5 to 6.5 μmol m^−-2 s^−-1 from understory to canopy. Both chamber grown and field leaves showed large adjustments in photosynthetic efficiency at low light via reductions in dark respiration rates and increases in apparent quantum yields. Light saturation of all leaves occurred at or below 500 μmol m^−-2 s^−-1. Leaf thickness, specific leaf weight, and stomatal density increased to a greater extent than saturated photosynthesis with higher irradiance during growth or height in the canopy. As a result, there was a poor correspondence between leaf thickness and light-saturated photosynthesis on an area basis. It is concluded that Pentaclethra macroloba possesses the characteristics of a typical shade-tolerant species.     

DIFFERENCES IN LEAF STRUCTURE,CHLOROPHYLL, AND NUTRIENTS FOR THE UNDERSTORY TREE ASIMINA TRILOBA

To investigate differences in leaf structure, chlorophyll and nutrients on terminal branches of the understory tree Asimina triloba, the first (proximal) and the last (distal) leaves to develop in the spring were compared. Proximal leaf expansion was completed before the overstory canopy was fully closed but distal leaf expansion occurred during and after the development of the overstory canopy. Fully expanded proximal leaves were 76% smaller in area, were 18% thicker and had 36% more stomates per m of leaf area when compared to distal leaves. In addition, maximum stomatal conductance to water vapor was greater (150 vs. 120 mmol m^−-2s^−-1) and the minimum PPFD required for maximum conductance was higher (200 vs. 150 μmol m^−-2s^−-1) for the proximal leaves. Chlorophyll content was also greater for proximal leaves, but nitrogen and phosphorus contents were lower throughout the entire summer. Seasonal measurements indicated an increase in chlorophyll a content and reductions in nitrogen content throughout the summer growth period for leaves from both positions. The results suggest that distal and proximal leaves differed physiologically and that the measured differences were related to the changing irradiance environment during leaf development. The time of leaf expansion, as indicated by leaf position on the branch, may be an important consideration when examining the water and photosynthetic relations of understory trees.     

Plant transpiration at high elevations: Theory,field measurements,and comparisons with desert plants

Summary The influence of elevational changes on plant transpiration was evaluated using leaf energy balance equations and well-known elevational changes in the physical parameters that influence water vapor diffusion. Simulated transpirational fluxes for large leaves with low and high stomatal resistances to water vapor diffusion were compared to small leaves with identical stomatal resistances at elevations ranging from sea level to 4 km. The specific influence of various air temperature lapse rates was also tested. Validation of the simulated results was accomplished by comparing actual field measurements taken at a low elevation (300 m) desert site with similar measurements for a high elevation (2,560 m) mountain research site. Close agreement was observed between predicted and measured values of transpiration for the environmental and leaf parameters tested.Substantial increases in solar irradiation and the diffusion coefficient for water vapor in air (D _wv) occurred with increasing elevation, while air and leaf temperatures, the water vapor concentration difference between the leaf and air, longwave irradiation, and the thermal conductivity coefficient for heat in air decreased with increasing elevation. These changes resulted in temperatures for sunlit leaves that were further above air temperature at higher elevations, especially for large leaves. For large leaves with low stomatal resistances, transpirational fluxes for low-elevation desert plants were close to those predicted for high-elevation plants even though the sunlit leaf temperatures of these mountain plants were over 10°C cooler. Simulating conditions with a low air temperature lapse rate (0.003° C m^-1 and 0.004° C m^-1) resulted in predicted transpirational fluxes that were greater than those calculated for the desert site. Transpiration for smaller leaves decreased with elevation for all lapse rates tested (0.003° C m^-1 to 0.010° C m^-1). However, transpirational fluxes at higher elevations were considerably greater than expected for all leaves, especially larger leaves, due to the strong influence of increased solar heating and a greater D _wv. These results are discussed in terms of similarities in leaf structure and plant habit observed among low-elevation desert plants and high-elevation alpine and subalpine plants.     

The Effect of Leaf Water Potential, Leaf Temperature and Light Intensity on Leaf Diffusion Resistance and the Transpiration of Leaves of Malus sylvestris

The relationship between leaf resistance to water vapour diffusion and each of the factors leaf water potential, light intensity and leaf temperature was determined for leaves on seedling apple trees (Malus sylvestris Mill. cv. Granny Smith) in the laboratory. Leaf cuticular resistance was also determined and transpiration was measured on attached leaves for a range of conditions. Leaf resistance was shown to be independent of water potential until potential fell below — 19 bars after which leaf resistance increased rapidly. Exposure of leaves to CO₂-free air extended the range for which resistance was independent of water potential to — 30 bars. The light requirement for minimum leaf resistance was 10 to 20 W m^?2 and at light intensities exceeding these, leaf resistance was unaffected by light intensity. Optimum leaf temperature for minimum diffusion resistance was 23 ± 2°C. The rate of change measured in leaf resistance in leaves given a sudden change in leaf temperature increased as the magnitude of the temperature change increased. For a sudden change of 1°C in leaf temperature, diffusion resistance changed at a rate of 0.01 s cm^?1 min^?1 whilst for a 9°C leaf temperature change, diffusion resistance changed at a rate of 0.1 s cm^?1 min^?1. Cuticular resistance of these leaves was 125 s cm^?1 which is very high compared with resistances for open stomata of 1.5 to 4 s cm^?1 and 30 to 35 s cm^?1 for stomata closed in the dark. Transpiration was measured in attached apple leaves enclosed in a leaf chamber and exposed to a range of conditions of leaf temperature and ambient water vapour density. Peak transpiration of approximately 5 × 10^?6 g cm^?2 s^?1 occurred at a vapour density gradient from the leaf to the air of 12 to 14 g m^?3 after which transpiration declined due presumably to increased stomatal resistance. Leaves in CO₂-free air attained a peak transpiration of 11 × 10^?6 g cm^?2 s^?1 due to lower values of leaf resistance in CO₂ free air. Transpiration then declined in these leaves due to development of an internal leaf resistance (of up to 2 s cm^?1). The internal resistance was masked in leaves at normal CO₂ concentrations by the increase in stomatal resistance.     

COMPARISON OF INTRASPECIFIC VARIATIONS IN THE REPRODUCTION AND PHOTOSYNTHESIS OF AN UNDERSTORY HERB,ARNICA CORDIFOLIA

Intraspecific variations in the reproduction of individual ramets and monospecific patches of the understory herb Arnica cordifolia Hook. were compared with variations in photosynthesis and understory light conditions. Ramets and patches were compared from three microhabitats (open, intermediate, and shaded) that differed in daily integrated irradiance. Individual ramets from open microhabitats (> 12 MJ m⁻² d⁻¹) had 23% more total dry wt and produced twice as many seeds, when compared to ramets from shaded locations (< 5 MJ m⁻² d⁻¹). In addition, monospecific patches from open locations were 63% more dense, and estimates of seed and vegetative patch reproductive effort were 4 and 2 times greater, respectively, when compared to shaded plant patches. For all measurements, ramets and patches from intermediate understory locations (6–10 MJ m⁻² d⁻¹) were intermediate in reproductive capacity between those of open and shaded locations. In addition, A. cordifolia seeds from open microhabitats germinated significantly better (45%) than either intermediate or shaded location seeds under high light and only seeds from shaded microhabitats germinated (14%) in the dark. Compared to shaded location plants, the greater total dry weight and seed production of individual ramets and the greater estimated reproductive effort of patches from open locations corresponded to a greater maximum photosynthetic rate (16.9 μmoles m⁻² s⁻¹) and daily carbon gain (12.2 g m⁻² d⁻¹). Possibly, a greater photosynthetic capacity may make more photosynthetic resources available for reproduction by A. cordifolia plants in open locations. Thus, intraspecific variation in physiology may contribute to intraspecific variation in reproduction.     

RNA Synthesis in Spring and Winter Wheat during Cold Acclimation

The factors responsible for the low transpiration rates of citrus were investigated. Leaf resistance to water vapor exchange by orange seedlings (Citrus sinensis L. cv. Koethen) including a substantial boundary layer resistance, was as low as 1 s cm⁻¹ in humid air. Leaf resistance of well watered plants increased to values as large as 5 s cm⁻¹ when the difference in absolute humidity between leaf and air was increased. Leaf resistance was only slightly influenced by temperature between 20 and 30°C providing the humidity difference between leaf and air was kept constant. Leaf resistance increased when leaf temperature was increased between 20 and 30°C when the absolute humidity external to the leaf was kept constant. Increased humidity differences resulted in greater increases in leaf resistance during initial experiments than when the experiments were repeated with the same leaves indicating acclimation by the plant. It was concluded that the effects of humidity differences on leaf resistance are partially responsible for the low transpiration rates of citrus.     

Vegetation effects on microclimate in lowland tropical forest in Costa Rica

The temperature and atmospheric humidity in a tropical lowland rain forest in Costa Rica were measured in order to assess the microclimate in different forest environments. Two disturbed sites, a single tree fall gap (400 m²) and an 0.5 hectare clearing, were compared for periods up to two years after disturbance. Two locations in primary forest, the canopy and understory, were also monitored. Temperatures were highest in the clearing, intermediate in the canopy and gap which were similar, and lowest in the understory. Vapor pressure deficits (VPD) were highest in the clearing, followed by the canopy, the gap and the understory. With regrowth of the vegetation in the gap and clearing sites, the temperatures and vapor pressure deficits significantly decreased. After 1 year, the microclimate at seedling height in the clearing resembled that of the gap, and after two years the microclimate of the gap was very similar to that of the understory. Seasonal differences in temperature and VPD were small compared to differences caused by changes in the stature of the vegetation.     

An In Situ Leaf and Branch Warming Experiment in the Amazon

Leaves and branches of mature trees, lianas, and gap species were warmed in an Amazonian forest for 4 mo to observe the effect of warming on photosynthesis, stomatal conductance, and transpiration. Electric resistance heaters increased air temperatures near the leaves by approximately 2°C. Sunlit leaf temperatures increased by 2–3°C on average, but during some periods leaf temperatures increased by >5°C. Maximum photosynthesis (A_max) decreased significantly in the warmed leaves vs. the control leaves over the 13‐wk study period with an average decrease in A_max of 1.4 μmol/m²s (19% decrease from a mean A_max of 7.2 μmol/m²s) when measured at 30°C and there were no signs of acclimation to higher temperatures within existing leaves. The decline in A_max was likely due to irreversible temperature damage caused by very high leaf temperatures and not due to C_i limitation of carboxylation. Warming had a larger negative impact on A_max in canopy level tree species than other tested functional groups such as lianas or gap species. Transpiration did not significantly increase in the warmed leaves compared with the control group. This study indicates that increased temperatures due to global warming could potentially decrease future tropical forest carbon uptake by a significant amount. Abstract in Portuguese is available at http://www.blackwell‐synergy.com/loi/btp .     

Leaf δ13C in Pinus resinosa trees and understory plants: variation associated with light and CO2 gradients

 Our objective was to evaluate the relative importance of gradients in light intensity and the isotopic composition of atmospheric CO₂ for variation in leaf carbon isotope ratios within a Pinus resinosa forest. In addition, we measured photosynthetic gas exchange and leaf carbon isotope ratios on four understory species (Dryopteris carthusiana, Epipactus helleborine, Hieracium floribundum, Rhamnus frangula), in order to estimate the consequence of the variation in the understory light microclimate for carbon gain in these plants. During midday, CO₂ concentration was relatively constant at vertical positions ranging from 15 m to 3 m above ground. Only at positions below 3 m was CO₂ concentration significantly elevated above that measured at 15 m. Based on the strong linear relationship between changes in CO₂ concentration and δ¹³C values for air samples collected during a diurnal cycle, we calculated the expected vertical profile for the carbon isotope ratio of atmospheric CO₂ within the forest. These calculations indicated that leaves at 3 m height and above were exposed to CO₂ of approximately the same isotopic composition during daylight periods. There was no significant difference between the daily mean δ¹³C values at 15 m (–7.77‰) and 3 m (–7.89‰), but atmospheric CO₂ was significantly depleted in ¹³C closer to the ground surface, with daily average δ¹³C values of –8.85‰ at 5 cm above ground. The light intensity gradient in the forest was substantial, with average photosynthetically active radiation (PAR) on the forest floor approximately 6% of that received at the top of the canopy. In contrast, there were only minor changes in air temperature, and so it is likely that the leaf-air vapour pressure difference was relatively constant from the top of the canopy to the forest floor. For red pine and elm tree samples, there was a significant correlation between leaf δ¹³C value and the height at which the leaf sample was collected. Leaf tissue sampled near the forest floor, on average, had lower δ¹³C values than samples collected near the top of the canopy. We suggest that the average light intensity gradient through the canopy was the major factor influencing vertical changes in tree leaf δ¹³C values. In addition, there was a wide range of variation (greater than 4‰) among the four understory plant species for average leaf δ¹³C values. Measurements of leaf gas exchange, under natural light conditions and with supplemental light, were used to estimate the influence of the light microclimate on the observed variation in leaf carbon isotope ratios in the understory plants. Our data suggest that one species, Epipactus helleborine, gained a substantial fraction of carbon during sunflecks. Received: 21 March 1996 / Accepted: 13 August 1996     

Effects of irradiance on growth and flowering in the shade plant, cineraria

Effects of total irradiance on growth and flowering were studied in cineraria cv. Cindy Blue grown under warm (mean 21°C) glasshouse conditions. Efficiency of light conversion for leaf and shoot dry weight increase were reduced from 0.08 to 0.02 as the mean daily light integral increased from 0.9 to 4.4 MJ m^-2 day^-1 but no significant difference in leaf area were associated with this. Specific leaf area decreased exponentially from 0.07 to 0.02 m²g¹ over the cumulative irradiance range 23 to 127 MJ m^-2 after the start of treatments and thereafter remained stable. A light integral of 19.2 MJ m^-2 were required for initiation of one leaf in plants grown under a daily integral of 4.4 MJ m^-2 day^-1, as compared with only 5.1 MJ m^-2day^-1 required per leaf in plants grown at less than 0.9 MJ m^-2day^-1. Neither chronological duration of juvenile development nor leaf number below the flower was affected by irradiance. However, as the rate of leaf initiation increased with irradiance up to 2.4 MJ m^-2day^-1 so the rate of progress to flower visibility increased linearly with irradiance over the same range. This rate then remained constant from 2.4 to 4.4 MJ m^-2day^-1. Length of the main flowering shoot decreased and the number of flowering shoots increased as irradiance increased from 0.9 to 2.4 MJ m^-2 day^-1 and then remained unchanged by further increases in irradiance.     

Annual carbon fixation in terrestrial populations of Nostoc commune (Cyanobacteria) from an Antarctic dry valley is driven by temperature regime

Nostoc commune Vaucher (a cyanobacterium) is a very conspicuous terrestrial primary producer in Victoria Land, continental Antarctica. Because polar ecosystems are considered to be especially sensitive to environmental changes, understanding the environmental constraints on net carbon (C) fixation by N. commune is necessary to determine the effects of environmental changes on the ecological functioning of ice‐free areas of the continent. A model describing net C fixation in terrestrial populations of N. commune in an Antarctic dry valley was constructed using field and laboratory measurements in which N. commune colonies were exposed to different combinations of incident irradiance (400–700 nm), temperature, and degree of desiccation. For desiccated N. commune mats with water content ≤ 30% saturation, net C fixation was highly variable between replicates and could not be modelled. However, for colonies at > 30% saturation, rates of net C fixation and dark respiration depended strongly on irradiance and temperature. Net C fixation reached a maximum rate of 21.6 μg C m^{− 2} s^{− 1} at irradiance of approximately 250 μmol m^{− 2} s^{− 1} and the optimum temperature of 20.5 °C. Agreement between predicted short‐term net C fixation and field and laboratory measurements allowed estimation of total seasonal fixation, using previously published environmental data. Annual net C fixation was estimated in the range 14.5–21.0 g C fixed m^{− 2}Nostoc mat, depending on year/season. Estimates for different seasons correlated with thermal time (accumulated hours above 0 °C during the year) rather than irradiance, in contrast to communities in local lacustrine environments, where irradiance is the main driver of primary productivity. In the terrestrial habitat, N. commune appears to compromise between an ability to capitalize on short periods of higher temperature and efficient utilization of lower irradiance at low temperature. The relationship between thermal time and net annual C fixation by N. commune is strongly linear.     

PHOTOSYNTHESIS IN AN EXTREME SHADE ENVIRONMENT: BENTHIC MICROBIAL MATS FROM LAKE HOARE, A PERMANENTLY ICE-COVERED ANTARCTIC LAKE

We investigated the composition of benthic microbial mats in permanently ice-covered Lake Hoare, Antarctica, and their irradiance vs. photosynthetic oxygen exchange relationships. Mats could be subdivided into three distinct depth zones: a seasonally ice-free “moat” zone and two under-ice zones. The upper under-ice zone extended from below the 3.5 m thick ice to approximately 13 m and the lower from below 13 m to 22 m. Moat mats were acclimated to the high irradiance they experienced during summer. They contained photoprotective pigments, predominantly those characteristic of cyanobacteria, and had high compensation and saturating irradiances (E_c and E_k) of 75 and 130 μmol photons·m⁻²·s⁻¹, respectively. The moat mats used light inefficiently. The upper under-ice community contained both cyanobacteria and diatoms. Within this zone, biomass (as pigments) increased with increasing depth, reaching a maximum at 10 m. Phycoerythrin was abundant in this zone, with shade acclimation and efficiency of utilization of incident light increasing with depth to a maximum of 0.06 mol C fixed·mol⁻¹ incident photons under light-limiting conditions. Precipitation of inorganic carbon as calcite was associated with this community, representing up to 50% of the carbon sequestered into the sediment. The lower under-ice zone was characterized by a decline in pigment concentrations with depth and an increasing prevalence of diatoms. Photosynthesis in this community was highly shade acclimated and efficient, with E_c and E_k below 0.5 μmol·m⁻²·s⁻¹ and 2 μmol·m⁻²·s⁻¹, respectively, and maximum yields of 0.04 mol C fixed·mol⁻¹ incident quanta. Carbon uptake in situ by both under-ice and moat mats was estimated at up to 100 and 140 mg·m⁻²·day⁻¹, based on the photosynthesis–irradiance curves, incident irradiance, and light attenuation by ice and the water column.     

Interactive Effects of CO2, Temperature,Irradiance, and Nutrient Limitation on the Growth and Physiology of the Marine Diatom Thalassiosira pseudonana (Coscinodiscophyceae)

The marine diatom Thalassiosira pseudonana was grown in continuous culture systems to study the interactive effects of temperature, irradiance, nutrient limitation, and the partial pressure of CO₂ (pCO₂) on its growth and physiological characteristics. The cells were able to grow at all combinations of low and high irradiance (50 and 300 μmol photons · m⁻² · s⁻¹, respectively, of visible light), low and high pCO₂ (400 and 1,000 μatm, respectively), nutrient limitation (nitrate-limited and nutrient-replete conditions), and temperatures of 10–32°C. Under nutrient-replete conditions, there was no adverse effect of high pCO₂ on growth rates at temperatures of 10–25°C. The response of the cells to high pCO₂ was similar at low and high irradiance. At supraoptimal temperatures of 30°C or higher, high pCO₂ depressed growth rates at both low and high irradiance. Under nitrate-limited conditions, cells were grown at 38 ± 2.4% of their nutrient-saturated rates at the same temperature, irradiance, and pCO₂. Dark respiration rates consistently removed a higher percentage of production under nitrate-limited versus nutrient-replete conditions. The percentages of production lost to dark respiration were positively correlated with temperature under nitrate-limited conditions, but there was no analogous correlation under nutrient-replete conditions. The results suggest that warmer temperatures and associated more intense thermal stratification of ocean surface waters could lower net photosynthetic rates if the stratification leads to a reduction in the relative growth rates of marine phytoplankton, and at truly supraoptimal temperatures there would likely be a synergistic interaction between the stresses from temperature and high pCO₂ (lower pH).     

Phospholipid synthesis and exchange in castor bean endosperm homogenates

Leaves on a bush of Hyptis emoryi Torr. varied in length from less than 1 cm when development occurred in full sunlight (e.g. 40 Mjoules m⁻²) to over 7 cm when the total daily solar irradiance was less than 3 Mjoules m⁻². The 1-cm sun leaves were 3-fold higher than the 7-cm shade leaves in chlorophyll per unit area, mesophyll thickness, and the internal to external leaf area ratio (A^mes/A). The higher A^mes/A caused a 1.2-cm leaf to have a 3-fold lower CO₂ liquid phase resistance than did a 7.1-cm leaf. Large thin shade leaves captured photosynthetically active radiation effectively (less than 7% passed through), but were not adapted to full sunlight. Specifically, when a 6.9-cm leaf was placed at 910 w m⁻² for 30 min, its temperature exceeded that of the air by nearly 8 C. For the common daytime air temperatures above 30 C for this desert shrub, large shade leaves would have temperatures far in excess of that optimum for photosynthesis for H. emoryi, 29 to 32 C.     

Differences in leaf thermoregulation and water use strategies between three co‐occurring Atlantic forest tree species

Given anticipated climate changes, it is crucial to understand controls on leaf temperatures including variation between species in diverse ecosystems. In the first study of leaf energy balance in tropical montane forests, we observed current leaf temperature patterns on 3 tree species in the Atlantic forest, Brazil, over a 10‐day period and assessed whether and why patterns may vary among species. We found large leaf‐to‐air temperature differences (maximum 18.3 °C) and high leaf temperatures (over 35 °C) despite much lower air temperatures (maximum 22 °C). Leaf‐to‐air temperature differences were influenced strongly by radiation, whereas leaf temperatures were also influenced by air temperature. Leaf energy balance modelling informed by our measurements showed that observed differences in leaf temperature between 2 species were due to variation in leaf width and stomatal conductance. The results suggest a trade‐off between water use and leaf thermoregulation; Miconia cabussu has more conservative water use compared with Alchornea triplinervia due to lower transpiration under high vapour pressure deficit, with the consequence of higher leaf temperatures under thermal stress conditions. We highlight the importance of leaf functional traits for leaf thermoregulation and also note that the high radiation levels that occur in montane forests may exacerbate the threat from increasing air temperatures.     

Water use by the desert cucurbit Citrullus colocynthis (L.) Schrad.

Summary The rates of water use and leaf surface conductance of Citrullus colocynthis (Cucurbitacea) were evaluated from measurements of the surface temperature and microenvironment of leaves. At desert sites in Saudi Arabia the transpiration rates reached 0.13–0.17 g m^-2 s^-1 and the leaf temperatures were always close to air temperature. Leaf models (dry) placed in the canopy were considerably warmer than the air. To investigate responses over a wider range of conditions, plants were grown in a controlled environment room. It was found that when conditions were made hotter than those that occurred in the desert, the stomatal conductance increased greatly. Transpiration rate attained 0.6 g m^-2 s^-1 and the leaves were up to seven degrees cooler than the air. The results suggest a finely-tuned control mechanism working like a switch when the leaves experience extreme conditions, and enabling the plant to avoid lethal temperatures.     

Stomatal conductance and transpiration of co-occurring seedlings with varying shade tolerance

Forest ecosystems exert a large influence on the hydrologic cycle through transpiration, with the majority supplied by saplings and adult trees. However, a measurable amount is also supplied by seedlings. The contribution of seedlings is dependent upon species, which is ultimately controlled by microclimate. The objectives of this study were to (1) observe meteorological conditions of two forest microclimates; and (2) assess the intra- and interspecific stomatal conductance and transpiration responses of naturally occurring seedlings of varying shade tolerance. Naturally established seedlings in a deciduous forest understory and an adjacent clearing were monitored throughout the 2008 growing season in southeastern Pennsylvania (39°49′N, 75°43′W). The understory microclimate conditions overall had a lower degree of variability and had consistently lower mean quantum flux densities, air temperature, vapor pressure deficit, volumetric water content, and soil temperature than the clearing plot. Understory seedlings of Fagus grandifolia Ehrh. (American beech) and Prunus serotina L. (black cherry) had significantly lower mean monthly rates of water loss (p = 0.05) than clearing seedlings [P. serotina and Liriodendron tulipifera L. (yellow poplar)]. Additionally, water loss by shade-grown P. serotina seedlings was significantly lower (p = 0.05) than by sun-grown seedlings. Physiological differences, specifically shade tolerance, played an important role in determining the rates of stomatal conductance and transpiration in the seedlings. To a lesser degree, microclimate variability also influenced water loss. The results of this study validate results obtained in previous studies conducted largely under controlled conditions. Field validations are critical to developing better models and forest management strategies.     

Shading by giant kelp canopy can restrict the invasiveness of Undaria pinnatifida (Laminariales,Phaeophyceae)

The spread of non-indigenous and invasive seaweeds has increased worldwide, and their potential effects on native seaweeds have raised concern. Undaria pinnatifida is considered among the most prolific non-indigenous species. This species has expanded rapidly in the Northeast Pacific, overlapping with native communities such as the iconic giant kelp forests (Macrocystis pyrifera). Canopy shading by giant kelp has been argued to be a limiting factor for the presence of U. pinnatifida in the understory, thus its invasiveness capacity. However, its physiological plasticity under light limitation remains unclear. In this work, we compared the physiology and growth of juvenile U. pinnatifida and M. pyrifera sporophytes transplanted to the understory of a giant kelp forest, to juveniles growing outside of the forest. Extreme low light availability compared to that outside (~0.2 and ~4.4 mol photon ⋅ m⁻² ⋅ d⁻¹, respectively) likely caused a “metabolic energy crisis” in U. pinnatifida, thus restricting its photoacclimation plasticity and nitrogen acquisition, ultimately reducing its growth. Despite M. pyrifera juveniles showing photoacclimatory responses (e.g., increases in photosynthetic efficiency and lower compensation irradiance, E_c), their physiological/vegetative status deteriorated similarly to U. pinnatifida, which explains the low recruitment inside the forest. Generally, our results revealed the ecophysiological basis behind the limited growth and survival of juvenile U. pinnatifida sporophytes in the understory.     

Effects of light regime on δ13C,photosynthesis and yield of field-grown banana (Musa sp., Musaceae)*

Banana plants (Musa sp., Musaceae) were grown for 2 years in the field in 12×20m plots under irradiance levels incident upon the canopy of 100, 81, 62 and 32% of sunlight. Time-integrated parameters such as leaf δ¹³C, yield and leaf mass to area ratio were linearly correlated with irradiance level (R²>0.9). In contrast, midday CO₂ assimilation and transpiration efficiency (A/g) decreased significantly and substomatal CO₂ concentrations (c_st) increased significantly only at the lowest irradiance levels (below 81% irradiance). Diurnal gas exchange measurements indicated that the linear response of the long-term parameters may be associated with the significant variations in photosynthetic activity among the irradiance treatments observed only in the early morning hours. The linear fit between yield and irradiance level (per cent of control) had a slope of 0.82 (with apparently constant yield to biomass ratio). These results directly demonstrate the significance of variations in incident light, such as may be associated with increasing cloudiness, on productivity of tropical plants such as the banana. The importance of using time-integrated indicators in general, and the reliability of using ¹³C discrimination in particular, in evaluating the responses of plants to changes in incident irradiance is demonstrated.     

Chilling damage in a changing climate in coastal landscapes of the subtropical zone: a case study from south Florida

Freeze events significantly influence landscape structure and community composition along subtropical coastlines. This is particularly true in south Florida, where such disturbances have historically contributed to patch diversity within the mangrove forest, and have played a part in limiting its inland transgression. With projected increases in mean global temperatures, such instances are likely to become much less frequent in the region, contributing to a reduction in heterogeneity within the mangrove forest itself. To understand the process more clearly, we explored the dynamics of a Dwarf mangrove forest following two chilling events that produced freeze‐like symptoms, i.e., leaf browning, desiccation, and mortality, and interpreted the resulting changes within the context of current winter temperatures and projected future scenarios. Structural effects from a 1996 chilling event were dramatic, with mortality and tissue damage concentrated among individuals comprising the Dwarf forest's low canopy. This disturbance promoted understory plant development and provided an opportunity for Laguncularia racemosa to share dominance with Rhizophora mangle. Mortality due to the less severe 2001 event was greatest in the understory, probably because recovery of the protective canopy following the earlier freeze was still incomplete. Stand dynamics were static over the same period in nearby unimpacted sites. The probability of reaching temperatures as low as those recorded at a nearby meteorological station (≤3 °C) under several warming scenarios was simulated by applying 1° incremental temperature increases to a model developed from a 42‐year temperature record. According to the model, the frequency of similar chilling events decreased from once every 1.9 years at present to once every 3.4 and 32.5 years with 1 and 4 °C warming, respectively. The large decrease in the frequency of these events would eliminate an important mechanism that maintains Dwarf forest structure, and promotes compositional diversity.