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1.
Maria Pilar Aguirre Paul Bloor Ursula Ramírez-Escobar Joaquín Ortego Pedro J. Cordero 《Conservation Genetics》2010,11(3):1119-1121
Eight polymorphic microsatellite markers were developed for the grasshopper Mioscirtus wagneri. Polymorphism at these loci was evaluated in 25 individuals from Central Spain. The number of alleles per locus ranged from
3 to 9 and their observed and expected heterozygosities ranged from 0.28 to 0.80 and 0.25 to 0.84, respectively. All genotypic
frequencies conformed to Hardy–Weinberg equilibrium expectations, with no evidence of genotypic linkage disequilibrium between
any pair of loci. These loci will be highly useful for the study of the population genetic structure and diversity of this
grasshopper species forming highly fragmented populations of great conservation concern. 相似文献
2.
In this study we characterized 10 polymorphic microsatellite markers for the land snail Cylindrus obtusus, an endemism of the Austrian Alps with a distribution in isolated populations above approximately 1,600 m. The microsatellite
loci were analyzed in 44 individuals from two populations. Number of alleles per locus ranged between two and eight. Observed
heterozygosity ranged between 0.00 and 1.00, and expected heterozygosity between 0.09 and 0.72. No significant linkage disequilibrium
was found between pairs of loci. One of the sampled populations (Dachstein) showed no deviation from Hardy–Weinberg equilibrium
and no presence of null alleles, whereas the other one (Schneeberg) did. These diverging results probably reflect differences
in population structure rather than characteristics of the microsatellite loci and underline the usefulness of these markers
for studying genetic diversity, population structure and differentiation in C. obtusus. 相似文献
3.
Li Wei Panyu Hua Weiwei Shao Cassandra M. Miller-Butterworth Shuyi Zhang 《Conservation Genetics》2009,10(3):677-679
We describe the first set of ten microsatellite markers isolated in Pipistrellus abramus. The number of alleles per locus ranged from 7 to 13. The observed and expected heterozygosities values ranged from 0.486
to 0.971 and from 0.752 to 0.876, respectively. Three loci revealed significant departure from Hardy–Weinberg equilibrium
and no linkage disequilibrium was found between loci pairs. These informative microsatellite markers will be a powerful molecular
tool for studying the population genetic structure of P. abramus, as well as other species of this genus. 相似文献
4.
Xin Zhan Hai-Yan Hu Cai-Huan Ke Song-Nian Hu De-Xiang Wang Fei Chen 《Conservation Genetics》2009,10(4):1185-1187
Eleven novel microsatellite markers were isolated from small abalone, Haliotis diversicolor Reeve. These loci were tested on 22 individuals from two different geographic populations. We identified a total of 162 alleles
from the 11 microsatellite loci. All of the loci were highly polymorphic. Polymorphism information content (PIC) is ranging
from 0.7276 to 0.9163. Observed and expected heterozygosities ranged from 0.2727 to 1.0000 and from 0.7738 to 0.9429, respectively.
Three loci deviated significantly from Hardy–Weinberg equilibrium. No pairs of loci displayed linkage disequilibrium. These
polymorphic markers will be used to analyze population structure, genetic diversity and construct a genetic linkage map.
Xin Zhan and Hai-Yan Hu contribute equally to this study. 相似文献
5.
Changwei Shao Songlin Chen Genbo Xu Xiaolin Liao Yongsheng Tian 《Conservation Genetics》2009,10(3):623-625
Chinese sea perch (Lateolabrax maculates) is one of the most important commercial species of mariculture in China. In this study, we constructed a repeat-enriched
genomic DNA library of L. maculates. Eighteen dinucleotide microsatellite markers were characterized by genotyping 32 samples. The number of alleles ranged from
three to nine, and the observed and expected heterozygosities ranged from 0.4516 to 1.0000 and from 0.4045 to 0.8676, respectively.
Significant deviations from Hardy–Weinberg expectations were detected at four loci and linkage disequilibrium between two
loci was significant after applying Bonferroni correction. The 18 highly polymorphic microsatellite markers should provide
sufficient level of genetic diversity to investigate the population structure and evaluate the breeding strategy in L. maculates. 相似文献
6.
Taxus sumatrana (Miq.) de Laub. (Taxaceae) is an endangered conifer with a scattered distribution in central Taiwan. In this study, we described
the development of 12 microsatellite loci in T. sumatrana for genetic studies. These new markers were tested in nine individuals of the rare species. The number of alleles ranged
from 3 to 13 and expected heterozygosity from 0.627 to 0.948. Eleven of twelve loci are significantly deviated from Hardy–Weinberg
expectations due to the heterozygote deficiency. 相似文献
7.
R. C. Garrick C. A. Meadows J. D. Nason A. I. Cognato R. J. Dyer 《Conservation Genetics》2009,10(4):1177-1179
We report eight new co-dominant nuclear markers for population genetics of the bark beetle Araptus attenuatus Wood. Several loci include introns from low-copy genes, and four cross-amplify in one or more related genera. The markers
show moderate levels of polymorphism (2–19 alleles per locus), and no loci showed significant deviations from Hardy–Weinberg
or linkage equilibrium across both of the two populations examined, consistent with Mendelian inheritance patterns. 相似文献
8.
Liang Zhang Xu Liang Zhihe Zhang Fujun Shen Wenping Zhang Kun Wei Zhi Yang Rong Hou Bisong Yue Hiroshi Kamata Ken Okabayashi Shigeo Ohba Hideo Kiba Shigehisa Tsumagari Tsuneo Sato Kiichi Kanayama Injen Pan Toshi Watanabe 《Conservation Genetics》2008,9(3):787-790
Ten polymorphic microsatellite loci were characterized from two genomic DNA-enriched libraries of the red panda (Ailurus fulgens). The number of observed alleles among 35 samples of red pandas ranged from five to 12. Observed and expected heterozygosities
were 0.286–0.971 and 0.443–0.894, and the mean polymorphic information content was 0.712. All loci followed Hardy–Weinberg
expectations except Aifu-14 and Aifu-16, which may due to the presence of inbreeding or null alleles. Three pairs of loci
exhibited significant linkage disequilibrium after Bonferroni correction for multiple comparisons. These microsatellites would
be useful to strengthen population management, genetic diversity exploration, and demographic history speculation of this
species. 相似文献
9.
Tsurng-Juhn Huang Yi-Yen Chen Yen-Ping Li Cheng-Yu Hung Tzen-Yuh Chiang Chang-Hung Chou 《Conservation Genetics》2008,9(5):1389-1391
Pedicularis verticillata L. is a highly valuable herb for traditional Chinese medical treatment. In this report, 11 microsatellite loci from P. verticillata were isolated. The simple sequence repeat (SSR) markers were screened in 23 samples of wild populations of P. verticillata, and eight samples from its sister P. ikomai. The number of alleles ranged from 4 to 13, and value of expected (H
E) and observed (H
0) heterozygosity was 0.62609–0.89662 and 0–0.95652, respectively. All loci were significantly deviated from Hardy–Weinberg
expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed
species. The markers amplified well in the two species are useful for examining genetic diversity and population genetic structure,
which, in turn, can provide information for establishing conservation strategy for these endangered species. 相似文献
10.
The spiny spider crab Maja brachydactyla in an important fishery resource throughout its distribution range (Northeast Atlantic). Here we describe the isolation of
nine microsatellite loci for this species. These new markers were tested in 20 crabs from NW Spain. The number of alleles
ranged from 3 to 20 and expected heterozigosity from 0.57 to 0.95. All loci followed Hardy–Weinberg expectations except mMb33
and mMb307, which could be affected by null alleles. Some of these loci seem to be better fitted by a multi-step substitution
process. 相似文献
11.
We describe the isolation and characterization of 13 polymorphic microsatellite loci in the endangered Monimopetalum chinense (Celastraceae) using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol in this paper. The 13 loci
were tested in 20 individuals from two populations. The number of alleles per locus varied from 2 to 8, with an average of
3.8. The expected and observed heterozygosities ranged from 0.05 to 0.87 and from 0.05 to 0.55, respectively. Two of the 13
loci were biased from Hardy–Weinberg equilibrium. No significant linkage disequilibrium was found among all loci. These polymorphic
SSR markers will be useful in studying population genetics of M. chinense. 相似文献
12.
Changwei Shao Genbo Xu Lei Wang Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(3):717-719
Given the ecological and commercial importance of the summer flounder (Paralichthys dentatus), there is a surprising paucity of information on the molecular genetics of this species. Some studies published to date
are concentrated on the reproduction biology. To address this shortcoming, a microsatellite-enriched genomic DNA library of
P. dentatus was generated and screened by sequencing. Twelve dinucleotide microsatellite loci were characterized by genotyping 24 samples.
The observed number of alleles ranged from three to thirteen with an average of 8.25, while the effective number of alleles
ranged from 2.21 to 8.28 with an average of 5.06. The observed and expected heterozygosities ranged from 0.0833 to 0.9583
and from 0.5594 to 0.8980, respectively. Significant deviations from Hardy–Weinberg expectations were detected at three loci
and linkage disequilibrium between two loci was significant after applying Bonferroni correction. In cross-species amplification,
three species showed at least two polymorphic loci. The 12 highly polymorphic microsatellite markers represent a powerful
molecular tool, which will allow for detailed population genetic analyses on this important marine fish. 相似文献
13.
A microsatellite-enriched genomic DNA library of Navodon septentrionalis was generated and screened by sequencing. Ten dinucleotide microsatellite loci were characterized by genotyping 24 samples.
The observed number of alleles ranged from two to seven with an average of 4.40, while the effective number of alleles ranged
from 1.49 to 5.70 with an average of 3.31. The observed and expected heterozygosities ranged from 0.2917 to 0.9167 and from
0.3369 to 0.8422, respectively. No significant linkage disequilibrium between pairs of loci was found, but one loci significantly
deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci would be useful
for investigating genetic population structure and molecule-assisted breeding for N. septentrionalis. 相似文献
14.
Chiao-Chuan Han Cheng-Sheng Chang Tzen-Yuh Chiang Ping-Han Chung Hung-Du Lin 《Conservation Genetics》2009,10(4):1065-1068
Caridina gracilipes, an atyid shrimp of Taiwan, inhabits the fresh and brackish waters. Its life history can be classified into amphidromous
and landlocked types. Recent human’s transportation has caused concerns of genetic contamination between these long evolved
lineages and ecological instabilities. Molecular markers with high sensitivities are required for detecting the possible introgression
due to human disturbance. In the study, we described the development of 15 polymorphic microsatellite loci in C. gracilipes for genetic and ecological studies. These new markers were tested in 20 individuals of three populations. The number of alleles
ranged from 6 to 17. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.653 to 0.954, averaged at 0.865, and from 0.000 to 0.4, averaged at 0.146, respectively.
All loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. Significant genetic
differentiation among populations was detected based on F
ST values, which were estimated from 0.82 to 0.88.
Chiao-Chuan Han and Cheng-Sheng Chang are contributed equally to this work. 相似文献
15.
Guijing Ren Jingjie Hu Zhenmin Bao Xin Jiang Tianxiang Gao 《Conservation Genetics》2009,10(6):1837-1839
Eleven polymorphic microsatellite markers were developed to delineate population structure of Ammodytes personatus. These markers had between 8 and 27 alleles. Expected heterozygosity ranged from 0.818 to 0.965, whereas the observed heterozygosity
ranged from 0.441 to 0.886. Five of the eleven markers conformed to Hardy–Weinberg equilibrium. We believe the markers will
be useful for genetic diversity study of A. personatus. 相似文献
16.
Selvaraju Kanagarajan Saraladevi Muthusamy Emily Chin-Fun Chen Hsin-Sheng Tsay 《Conservation Genetics》2009,10(5):1487-1490
We developed and characterized 8 polymorphic microsatellite loci or simple sequence repeats from the expressed sequence tags
database of the medicinal fungus, Antrodia cinnamomea. Variability was assessed in a panel of 23 strains collected from various regions of Taiwan. The number of alleles per polymorphic
locus ranged from two to seven loci with an average of 4.375 alleles per locus. The observed and expected heterozygosity values
ranged from 0.130 to 0.783 and from 0.122 to 0.809, respectively. No significant Hardy–Weinberg equilibrium was detected (P < 0.01) at all loci. The EST-SSR markers developed in the present study can be used for strain identification and population
genetic studies in A. cinnamomea. 相似文献
17.
Hung-Du Lin Teh-Wang Lee Fang-Jiao Lin Chung-Jian Lin Tzen-Yuh Chiang 《Conservation Genetics》2008,9(4):945-947
Pararasbora moltrechti (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the central Taiwan. In order to undertake
a conservation program for this species in Taiwan, it is essential to evaluate its genetic diversity and population structure.
For this purpose, we described the development of eight microsatellite loci in P. moltrechti for genetic studies. These new molecular markers were tested in 36 individuals of the rare species. The number of alleles
per locus ranged from 8 to 14. As the expected heterozygosity varied from 0.770 to 0.932, all microsatellite loci, however,
were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency.
Hung-Du Lin and Teh-Wang Lee contributed equally. 相似文献
18.
Chi-Chun Huang Kuo-Hsiang Hung Tsai-Wen Hsu Kuo-Hsiung Wang Chi-Yung Lin Tzen-Yuh Chiang 《Conservation Genetics》2008,9(5):1333-1335
Fatsia polycarpa Hayata (Araliaceae) is an element of evergreen forests at middle elevations in Taiwan, also representing the southern limitation
in distribution of the genus. In this study, we described the development of 11 microsatellite loci from F. polycarpa in Taiwan for genetic studies. These new markers were tested in 20 individuals of 5 populations of the woody species. The
number of alleles ranged from 9 to 18. The expected (H
E
) and observed (H
O
) heterozygosities were 0.80–0.95 and 0.10–0.75, respectively. Six of the 11 microsatellite loci are significantly deviated
from Hardy–Weinberg expectations likely due to the population structure within samples. No linkage disequilibrium were observed
from pairwise comparisons of loci.
Chi-Chun Huang, Kuo-Hsiang Hung, and Tsai-Wen Hsu contributed equally to this article. 相似文献
19.
Chi-Hang Chan Yudi Zhao Mei-Yan Cheung Geoffrey K. Chambers 《Conservation Genetics》2008,9(4):1067-1070
The kakerori (Pomarea dimidiata) is an endangered forest bird in the Cook Islands, South Pacific. We have developed 10 microsatellite markers using kakerori
feathers as the DNA source. Seven of these loci were found to be polymorphic in 42 individuals examined. The number of alleles
per locus in the polymorphic loci varied from 3 to 5. Observed and expected heterozygosity ranges were 0.57–0.74 and 0.50–0.74,
respectively. All loci isolated conformed to Hardy–Weinberg equilibrium expectations. We believe these loci will be useful
in studying kakerori conservation genetics, and our success in developing microsatellite markers from feather samples will
encourage the use of less invasive sample sources in microsatellite isolation studies. 相似文献
20.
Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae) 总被引:1,自引:0,他引:1
Kuo-Hsiang Hung Tzen-Yuh Chiang Chi-Te Chiu Tsai-Wen Hsu Chuan-Wen Ho 《Conservation Genetics》2009,10(5):1377-1380
Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine
microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of
alleles ranged from 3 to 8. The expected (H
E) and observed (H
O) heterozygosities were 0.37–0.78 and 0.05–0.52, respectively. All microsatellite loci are significantly deviated from Hardy–Weinberg
expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed
from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its
closely related species, M. floridulus. 相似文献