Identification and characterization of ten polymorphic microsatellite loci in the red panda Ailurus fulgens |
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| Authors: | Liang Zhang Xu Liang Zhihe Zhang Fujun Shen Wenping Zhang Kun Wei Zhi Yang Rong Hou Bisong Yue Hiroshi Kamata Ken Okabayashi Shigeo Ohba Hideo Kiba Shigehisa Tsumagari Tsuneo Sato Kiichi Kanayama Injen Pan Toshi Watanabe |
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| Institution: | (1) Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, 610081, China;(2) Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Sichuan, 610064, China;(3) Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan |
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| Abstract: | Ten polymorphic microsatellite loci were characterized from two genomic DNA-enriched libraries of the red panda (Ailurus fulgens). The number of observed alleles among 35 samples of red pandas ranged from five to 12. Observed and expected heterozygosities
were 0.286–0.971 and 0.443–0.894, and the mean polymorphic information content was 0.712. All loci followed Hardy–Weinberg
expectations except Aifu-14 and Aifu-16, which may due to the presence of inbreeding or null alleles. Three pairs of loci
exhibited significant linkage disequilibrium after Bonferroni correction for multiple comparisons. These microsatellites would
be useful to strengthen population management, genetic diversity exploration, and demographic history speculation of this
species. |
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| Keywords: | Red Panda Ailurus fulgens Microsatellite (CA)n (CAA)n |
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