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1.
A novel halophilic, filamentous actinobacterium, designated TRM 40136 T, was isolated from a hypersaline habitat in Xinjiang Province, north-west China. The strain is aerobic, Gram-positive, halophilic,
and the optimum NaCl concentration for growth is 10–15% (w/v). The whole-cell sugar pattern consists of xylose, glucose and
arabinose. The predominant menaquinone is MK-6 (51.2%) and the major fatty acids are anteiso-C15:0 (35.2%), anteiso-C17:0
(15.9%) and iso-C15:0 (13.7%). The phospholipid pattern consists of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine
and two unknown phospholipids. The G + C content of the genomic DNA is 68.9 mol%. Phylogenetic analysis showed that strain
TRM 40136 T had 16S rRNA gene sequence similarity of 96.1% with the closest described species Actinopolyspora mortivallis, and it can be distinguished from all species in the genus Actinopolyspora by using these data of polyphasic taxonomy study. On the basis of the polyphasic evidence, the strain TRM 40136 T should be designated as a novel species of the genus Actinopolyspora for which the name Actinopolyspora xinjiangensis sp.nov. is proposed. The type strain is TRM 40136 T (=CCTCC AA 209080 T = KCTC 19656 T). 相似文献
2.
A novel actinomycete strain, designated TRM 40133 T, was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was
determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain
showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133 T and Saccharopolyspora qijiaojingensis YIM 91168 T (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic
phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main
menaquinone was MK-9(H 6) and MK-9(H 4). No mycolic acid was detected. The predominant cellular fatty acids were iso-C 16:0 and anteiso-C 17:0. The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133 T had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133 T therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133 T (=KCTC 19987 T =CCTCC AA 2010012 T). 相似文献
3.
During a screening for novel and useful actinobacteria in desert animal, a new actinomycete was isolated and designated strain TRM63209T. The strain was isolated from in vivo of a Blattella germanica in Tarim University in Alar City, Xinjiang, north-west China. The strain was found to exhibit an inhibitory effect on biofilm formation by Candida albicans ATCC 18,804. The strain was observed to form abundant aerial mycelium, occasionally twisted and which differentiated into spiral spore chains. Spores of TRM63209T were observed to be oval-shaped, with a smooth surface. Strain TRM63209T was found to grow optimally at 28 °C, pH 8 and in the presence of 1% (w/v) NaCl. The whole-cell sugars of strain TRM63209T were rhamnose ribose, xylose, mannose, galactose and glucose, and the principal polarlipids were found to be diphosphatidylglycerol, phos-phatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, phosphatidylinositol and an unknown phospholipid(L). The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The predominant menaquinone was found to be MK-9(H6) (14.64%), MK-9(H2) (19.65%), MK-9(H8) (22.34%), MK-10(H2) (25.37%). The major cellular fatty acids were identified as iso-C16:0, 16:0, anteiso-C15:0, anteiso-C17:0, iso-C15:0 and Sum in Feature 3. Analysis of the 16S rRNA sequence showed that strain TRM63209T exhibits high sequence similarity to Streptomyces bungoensis strain DSM 41781T 98.20%. A multi-locus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) and phylogenomic analysis also illustrated that strain TRM63209T should be assigned to the genus Streptomyces. The DNA G?+?C content of the strain was determined to be 70.2 mol%. Average nucleotide identity (ANI) between strain TRM63209T and S. bungoensis DSM 41781T, Streptomyces phyllanthi PA1-07T, Streptomyces longwoodensis DSM 41677T and Streptomyces caeruleatus NRRL B-24802T were 82.76%, 82.54%, 82.65%, 84.02%, respectively. Digtal DNA-DNA (dDDH) hybridization were 26.30%, 25.10%, 26.20%, 29.50%, respectively. Therefore, it is concluded that strain TRM63209T represents a novel species of the genus Streptomyces, for which the name Streptomyces blattelae is proposed. The type strain is TRM63209T (CCTCC AA 2018093T?=?LMG 31,403?=?TRM63209T). 相似文献
4.
A novel halophilic, filamentous actinomycete, designated TRM 4064 T, was isolated from a hypersaline habitat in Sichuan Province, China. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of strain TRM 4064 T showed that it was most closely related to Actinopolyspora mortivallis (99.1 % sequence similarity). The sequence similarities between strain TRM 4064 T and other Actinopolyspora species with validly-published names were <97.0 %. However, it had relatively low mean values for DNA–DNA relatedness with the A. mortivallis DSM 44261 T (23.2 %). Optimal growth occurred at 37 °C, pH 7.0 and in the presence of 13 % (w/v) NaCl. The whole-cell sugar pattern consists of xylose, glucose, ribose and arabinose. The predominant menaquinones are MK-10(H 4) (38.2 %), MK-9(H 4) (25.1 %), MK-9(H 2) (28.6 %) and MK-8(H 4) (7.3 %). The major fatty acids are anteiso-C 17:0 (36.9 %) and iso-C 17:0 (19.3 %). The diagnostic phospholipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylinositol (PI) and two unknown phospholipids. The G+C content of the genomic DNA of the type strain is 66.3 mol%. Strain TRM 4064 T therefore represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora dayingensis sp. nov. is proposed. The type strain is TRM 4064 T (= KCTC 19979 T = CCTCC AA 2010010 T). 相似文献
5.
A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, non-motile, non-sporulating, catalase-positive,
oxidase-negative, aerobic bacterium, designated strain JSM 070026 T, was isolated from non-saline forest soil in China. Growth occurred with 0–20% (w/v) NaCl (optimum, 2–4%) and at pH 6.0–10.5
(optimum, pH 8.0) and 5–40°C (optimum, 30°C). Good growth also occurred in the presence of 0–28% (w/v) KCl (optimum, 2–5%)
or 0–25% (w/v) MgCl 2·6H 2O (optimum, 1–4%). The peptidoglycan type was A4 α ( l-Lys–Gly– l-Glu). Cell-wall sugars contained mannose and xylose. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. Strain
JSM 070026 T contained menaquinone 8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol
as the major polar lipids. The DNA G + C content of strain JSM 070026 T was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JSM 070026 T was a member of the suborder Micrococcineae and most closely related to Yaniella flava YIM 70178 T (sequence similarity 99.4%) and Yaniella halotolerans YIM 70085 T (97.9%). The three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis, DNA–DNA
relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 070026 T represents a novel species of the genus Yaniella, for which the name Yaniella soli sp. nov. is proposed. The type strain is JSM 070026 T (=DSM 22211 T = KCTC 13527 T). 相似文献
7.
The taxonomic study of two Gram-negative, aerobic, non-pigmented bacteria KMM 9010 T and KMM 9023 T isolated from a sandy sediment sample collected from the Sea of Japan seashore was performed. On the basis of the nearly
complete 16S rRNA gene sequences, strains KMM 9010 T and KMM 9023 T clustered with the Roseobacter lineage (class Alphaproteobacteria) forming a distinct phylogenetic line adjacent to the genus Donghicola. Novel strains shared the highest sequence similarity of 96.4% to each other and lower than 96.1% similarities to other validly
named genera of the class Alphaproteobacteria. In both strains, ubiquinone Q-10 was found to be the major respiratory quinone; phosphatidylcholine, phosphatidylglycerol,
diphosphatidylglycerol, phosphatidic acid, and an unknown aminolipid were the major polar lipids and C 18:1ω7 c and 11-methyl C 18:1ω7 c were predominant fatty acids. The DNA G+C content was 60.5 mol% (KMM 9010 T) and 65.4 mol% (KMM 9023 T). Based on phenotypic properties and phylogenetic evidence, strains KMM 9010 T and KMM 9023 T should be classified as two novel species in a new genus, Poseidonocella gen. nov., with Poseidonocella pacifica sp. nov., the type species with the type strain KMM 9010 T (= NRIC 0794 T = JCM 17310 T), and Poseidonocella sedimentorum sp. nov. as the second species with the type strain KMM 9023 T (= NRIC 0796 T = JCM 17311 T). 相似文献
8.
A novel Gram-positive, aerobic, motile, hemolytic, endospore-forming and rod-shaped bacterium TW25 T was isolated from a dead ark clam during a mass mortality event on the South coast of Korea. The strain grew optimally at
30°C, at pH 8–9, and with 1% (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain TW25 T was associated with the genus Ornithinibacillus and that it was most closely related to the type strain of Ornithinibacillus californiensis (98.5% similarity). The dominant cellular fatty acids were iso-C15:0, anteiso-C15:0 and C16:0. The peptidoglycan amino acid
type was A4 β, containing l-ornithine and d-aspartic acid. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids, two unidentified
aminolipids and two unidentified lipids. The major respiratory quinone was menaquinone-7 (MK-7). The G + C content of genomic
DNA was 36.7 mol%. DNA–DNA hybridization experiments with related strains revealed lower than 11 ± 3% relatedness. Based on
this polyphasic taxonomic study, strain TW25 T represents a novel species in the genus Ornithinibacillus, for which the name Ornithinibacillus scapharcae sp. nov. is proposed. The type strain is TW25 T (=KACC 15116 T = JCM 17314 T). 相似文献
9.
A gram-negative, non-flagellated and ovoid- to rod-shaped bacterial strain, designated GSW-M15 T, was isolated from seawater on the southern coast of South Korea. Strain GSW-M15 T grew optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain GSW-M15 T belonged to the genus Roseovarius. Strain GSW-M15 T exhibited highest 16S rRNA gene sequence similarity values (98.3 and 97.5 %) to Roseovarius halotolerans HJ50 T and Roseovarius pacificus 81-2 T and 92.8-96.2 % sequence similarity values to the type strains of the other Roseovarius species. Strain GSW-M15 T contained Q-10 as the predominant ubiquinone and C 18:1 ω7 c and 11-methyl-C 18:1 ω7 c as the major fatty acids. The major polar lipids detected in strain GSW-M15 T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain GSW-M15 T was 62.9 mol% and its mean DNA–DNA relatedness values with R. halotolerans KCTC 22224 T and R. pacificus LMG 24575 T were 33 and 18 %, respectively. Differential phenotypic properties of strain GSW-M15 T, together with the phylogenetic and genetic distinctiveness, demonstrated that this strain is distinguishable from other Roseovarius species. On the basis of the data presented here, strain GSW-M15 T (=KCTC 23897 T = CCUG 62218 T) represents a novel species of the genus Roseovarius, for which the name Roseovarius litoreus sp. nov. is proposed. 相似文献
10.
A novel bacterium, strain blls-2 T was isolated from Pu’er tea. The isolate was Gram-positive, endospore-forming motile rod that grew at 15∼42°C and pH 6.0∼10.2.
The DNA G+C content was 48.3 mol%, the predominant isoprenoid quinone was MK-7, and the predominant cellular fatty acid was
anteiso-C15:0 (54.2%) followed by C16:0 (15.5%) and iso-C16:0 (8.2%). The polar lipid pattern of blls-2 T was characterized by the presence of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phy-logenetic
analysis based on 16S rRNA gene sequence showed that the strain was affiliated within the Paenibacillaceae. The strain was most closely related to Paenibacillus granivorans A30 T, with a similarity of 97.1%. Based on the phylogenetic and phenotypic characteristics of strain blls-2 T, the isolate is thought to represent a novel taxon in the genus Paenibacillus. The name Paenibacillus camelliae sp. nov. is proposed for the fermented tea isolate; the type strain is blls-2 T (= KCTC 13220 T= CECT 7361 T). 相似文献
11.
A novel actinobacterial strain, designated TRM SA0054 T, was isolated from the roots of Sophora alopecuroides grown in Alar, Xinjiang, north-west China, and characterised by a polyphasic taxonomic approach. Phylogenetic analysis showed that strain TRM SA0054 T has 16S rRNA gene sequence similarity of 98.22% with Streptomyces barkulensis RC 1831 T. Whole cell hydrolysates of strain TRM SA0054 T were found to contain ll-diaminopimelic acid as the diagnostic diamino acid and ribose, glucose and xylose as the major whole cell sugars. The major fatty acids were identified as iso-C 16:0, iso-C 16:1 G, anteiso-C 17:0, anteiso-C 15:0, C 16:0, anteiso-C 17:1 ω9c. The main menaquinones were determined to be MK-8 (H 4), MK-9 (H 6) and MK-9 (H 8). The polar lipids were identified as diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid. The G?+?C content of the genomic DNA was determined to be 73.04%. Strain TRM SA0054 T has a relatively low DNA–DNA relatedness value with Streptomyces barkulensis RC 1831 T as determined by calculating the average nucleotide identity value (ANI?=?84.1%). Based on the phenotypic, chemotaxonomic and phylogenetic data, it is concluded that strain TRM SA0054 T should be designated as a novel species of the genus Streptomyces, for which the name Streptomyces carminius sp. nov. is proposed, with type strain TRM SA0054 T (=?CCTCC AA 2016041 T?=?KCTC39903 T). 相似文献
12.
A Gram-positive, rod-shaped, motile and spore-forming bacterium, designated ZLD-8 T, was isolated from a desert soil sample collected from Xinjiang Province in north-west China, and subjected to a polyphasic
taxonomic analysis. This isolate grew optimally at 30°C and pH 7.0. It grew with 0–4% NaCl (optimum, 0–1%). Comparative 16S
rRNA gene sequence analysis showed that strain ZLD-8 T was closely related to members of the genus Bacillus, exhibiting the highest 16S rRNA gene sequence similarity to Bacillus kribbensis DSM 17871 T (98.0%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.3%. The DNA G + C content of strain ZLD-8 T was 40.1 mol%. The strain contained MK-7 as the predominant menaquinone. The diagnostic diamino acid in the cell-wall peptidoglycan
was meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
The major fatty acids (>5% of total fatty acids) were anteiso-C15:0 (39.56%), iso-C14:0 (25.69%), C16:1 ω7 c alcohol (10.13%) and iso-C15:0 (5.27%). These chemotaxonomic results supported the affiliation of strain ZLD-8 T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation
of strain ZLD-8 T from recognized Bacillus species. On the basis of the polyphasic evidence presented, strain ZLD-8 T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus
deserti sp. nov. is proposed. The type strain is ZLD-8 T (=CCTCC AB 207173 T = KCTC 13246 T). 相似文献
13.
Five strains (LN12, LN14 T, LN15 T, LN16 and LN17 T) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive
enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis
of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to
be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14 T and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14 T (= BCC 42666 T = NBRC 107780 T = CBS 12095 T). Strain LN15 T was assigned to be Candida chumphonensis sp. nov. (type strain LN15 T = BCC 42667 T = NBRC 107781 T = CBS 12096 T). Strain LN17 T represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17 T = BCC 42668 T = NBRC 107782 T = CBS 12097 T). 相似文献
14.
Three strains (LM008 T, LM068 and LM078 T), representing two novel yeast species were isolated from the phylloplane of three plant species by an enrichment technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal spacer region, the three strains were assigned as two novel Candida species. Strain LM008 T was assigned to be Candida sirachaensis sp. nov. (type strain LM008 T = BCC 47628 T = NBRC 108605 T CBS 12094 T) in the Starmerella clade. Two strains (LM068 and LM078 T) represent a single species in the Lodderomyces- Spathaspora clade for which the name Candida sakaeoensis sp. nov. is proposed with the type strain LM078 T = BCC 47632 T = NBRC 108895 T = CBS 12318 T. 相似文献
15.
A novel aerobic mesophilic bacterial strain SYBC-H1 T capable of degrading chitin was isolated and classified in this study. The strain exhibited strong chitinolytic activity
and was a Gram-negative, curved, rod-shaped, and motile bacterium. Growth of this strain was observed between 10 and 41°C
and between pH 3.5 and 9.5. The DNA G + C content of strain SYBC-H1 T was 53.25 mol%. The cellular fatty acids (>5%) were 12:0 iso 3-OH (5.87%), 16:0 (28.16%), and 18:1ω7c (20.48%). Phylogenetic
analysis based on 16S rRNA gene sequence similarity revealed that strain SYBC-H1 T belonged to the family Neisseriaceae, and was distantly related (95.0% similarity) to the genus Chitiniphilus. Its phenotype was unique and genetic and phylogenetic analysis experiments suggested that strain SYBC-H1 T represented the type strain (CGMCC 3438 T, ATCC BAA-2140 T) of a novel genus, for which the name Chitinolyticbacter meiyuanensis SYBC-H1 T gen. nov., sp. nov. was proposed. The highest enzymatic activity of chitinase (9.6 U/ml) was obtained at 72 h in 250 ml shake
flasks. The 16S rRNA gene sequence of SYBC-H1 T has been deposited in GenBank under the accession number GQ981314. 相似文献
16.
Three ascosporogenous yeast strains were isolated from the gut of the passalid beetle, Odontotaenius disjunctus, inhabiting on rotten oak trees. DNA sequence comparison and other taxonomic characteristics identified the strains as a
novel species in the genus Kazachstania. The name Kazachstania
intestinalis sp. nov. (type strain EH085 T = ATCC MYA-4658 T = CBS 11839 T) is proposed for the strains. The yeast is homothallic, producing persistent asci with 1–4 spheroidal ascospores. Molecular
phylogeny from ribosomal RNA gene sequences placed this novel species on the basal lineage of a clade including Kazachstania lodderae, Kazachstania exigua, Kazachstania martiniae, and other related Kazachstania spp., but none of those species was a close sister to K.
intestinalis. 相似文献
17.
A gram-negative, motile, straight to curved rod shaped, pink pigmented bacterium was isolated from a soil sample collected
from the rhizosphere of an Indian medicinal plant, Nerium indicum ( Chuvanna arali) and subjected to a detailed polyphasic taxonomic study. The strain, designated as IMTB-1969 T, matched with most of the phenotypic and chemotaxonomic properties of the genus Pontibacter and represents a novel species. The major fatty acids of the strain were monounsaturated iso/anteiso branched C17 fatty acids
(45.1%) and iso-C15:0 (16.5%). MK-7 was the predominant isoprenoid quinone. According to 16S rRNA gene sequence analysis,
strain IMTB-1969 T was indicated to belonged to the phylum Bacteroidetes and further phylogenetic analysis revealed that the strain IMTB-1969 T belongs to the family Cytophagaceae and genus Pontibacter. The highest 16S rRNA gene sequence similarity was with Pontibacter korlensis CCTCC AB 206081 T (97.2%) and lower sequence similarity was observed with other species in the genus Pontibacter (95.9–94.0%). DNA–DNA relatedness study of the strain IMTB-1969 T confirmed that it represents a novel species. The G+C content of the genomic DNA was 52.2 (±0.5) mol%. The results of physiological
and biochemical tests allowed the genotypic and phenotypic distinction of strain IMTB-1969 T from its closest phylogenetic relatives. The strain IMTB-1969 T should be classified as novel species of the genus Pontibacter, for which the name Pontibacter rhizosphera sp. nov. is proposed. The type strain is IMTB-1969 T (=MTCC 10673 T = DSM 24399 T). 相似文献
18.
During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 63101 T was isolated from the surface-sterilized roots of Artemisia annua L. collected from Xishuangbanna, Yunnan province, south-west China and characterized by using a polyphasic approach. The
strain formed well-differentiated aerial and substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequences showed
that strain YIM 63101 T belongs to the genus Pseudonocardia, with highest similarity to “ Pseudonocardia artemisiae YIM 63587 T” (99.4%). Sequence similarities between strain YIM 63101 T and the other Pseudonocardia species ranged from 97.0 ( Pseudonocardia saturnea IMSNU 20052 T) to 94.0% ( Pseudonocardia compacta IMSNU 20111 T). The chemotaxonomic characteristics, such as cell wall diaminopimelic acid, whole-cell sugars, fatty acid components and
the major menaquinones suggested that the organism belonged to the genus Pseudonocardia. The G + C content of the genomic DNA was 69.4 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic
data, including low DNA–DNA hybridization results, it is proposed that strain YIM 63101 T represents a novel species of the genus Pseudonocardia, named Pseudonocardia bannaensis sp. nov. The type strain is YIM 63101 T (= CCTCC AA 208077 T = DSM 45300 T). 相似文献
19.
An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated
from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23 T, was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic
analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23 T is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas
chromatography were iso-C 15:0, iso-C 17:0 and iso C 17:1 ω9c. The major respiratory quinone was MK-8. The DNA G + C content was 66.9 mol%. DNA–DNA hybridization measurements revealed
low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23 T represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23 T (=DSM 18496 T; LMG 23756 T). 相似文献
20.
A novel actinomycete strain, GW25-5 T, was isolated from a soil sample collected from the Fildes Peninsula, King George Island, West Antarctica. The strain was
characterized by white to grey aerial mycelia, which were differentiated to straight to flexuous spore chains, with rod-shaped
smooth spores. The cell wall of strain GW25-5 T contained LL-diaminopimelic acid (A 2pm) and traces of meso-A 2pm. Whole-cell sugars were galactose and minor amounts of mannose and glucose. The predominant menaquinones were MK-9(H 6) (49%), MK-9(H 8) (24%) and MK-9(H 4) (12%). The phospholipids contained DPG, PE, PI, PIM and PL(s). The major cellular fatty acids were iso-C 16:0 and anteiso-C 15:0. Genomic DNA G+C content of strain GW25-5 T was 70.0 mol%. BLAST result showed that strain GW25-5 has the 16S rRNA gene sequence highest similarity of 97.5% with members
of genus Streptomyces and phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. DNA–DNA relatedness values of strain GW25-5 T with the closest species of Streptomyces
purpureus LMG 19368 T and Streptomyces
beijiangensis YIM 6 T were significantly lower than 70% of the threshold value for the delineation of genomic species. A polyphasic taxonomic investigation
based on a judicious combination of genotypic and phenotypic characteristics revealed that the organism represents a novel
species of the genus Streptomyces. Thus, we propose strain GW25-5 T as the type strain of this novel species, Streptomyces
fildesensis (=CGMCC 4.5735 T = YIM 93602 T = DSM 41987 T = NRRL B 24828 T). 相似文献
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