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Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato 总被引:3,自引:0,他引:3
Hwang Bae Sohn Han Yong Lee Ju Seok Seo Choonkyun Jung Jae Heung Jeon Jeong-Han Kim Yin Won Lee Jong Seob Lee Jong-Joo Cheong Yang Do Choi 《Plant biotechnology reports》2011,5(1):27-34
Jasmonates control diverse plant developmental processes, such as seed germination, flower, fruit and seed development, senescence
and tuberization in potato. To understand the role of methyl jasmonate (MeJA) in potato tuberization, the Arabidopsis
JMT gene encoding jasmonic acid carboxyl methyltransferase was constitutively overexpressed in transgenic potato plants. Increases
in tuber yield and size as well as in vitro tuberization frequency were observed in transgenic plants. These were correlated
with JMT mRNA level––the higher expression level, the higher the tuber yield and size. The levels of jasmonic acid (JA), MeJA and
tuberonic acid (TA) were also higher than those in control plants. Transgenic plants also exhibited higher expression of jasmonate-responsive
genes such as those for allene oxide cyclase (AOC) and proteinase inhibitor II (PINII). These results indicate that JMT overexpression induces jasmonate biosynthesis genes and thus JA and TA pools in transgenic potatoes. This results in enhanced
tuber yield and size in transgenic potato plants. 相似文献
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Rongjia Mao Jing Chen Yuejun Chen Zhigang Guo 《Physiology and Molecular Biology of Plants》2018,24(5):715-727
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van Wees SC Luijendijk M Smoorenburg I van Loon LC Pieterse CM 《Plant molecular biology》1999,41(4):537-549
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Johan Memelink 《Phytochemistry》2009,70(13-14):1560-1570
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Keji Jiang Zhihua Liao Yan Pi Zhuoshi Huang Rong Hou Ying Cao Qing Wang Xiaofen Sun Kexuan Tang 《Molecular Biology》2008,42(3):381-390
Hyoscyamus niger L. is a medicinal plant which produces a class of jasmonate-responsive pharmaceutical secondary metabolites named tropane
alkaloids. As a family of signaling phytohormones, jasmonates play significant roles in the biosynthesis of many plant secondary
metabolites. In the jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important
step. Here we cloned a cDNA from H. niger, named HnAOC (GenBank accession no.: AY708383), which was 1044 bp long, with a 747-bp open reading frame (ORF) encoding a polypeptide
of 248 amino acid residues. Southern blot analysis indicated that it was a multicopy gene. RT-PCR analysis revealed that the
expression of HnAOC was regulated by various stresses and elicitors, with methyl-jasmonate showing the most prominent inducement. The characterization
of HnAOC would be helpful for improving the production of valuable secondary metabolites by regulating the biosynthesis of jasmonates.
The text was submitted by the authors in English. 相似文献
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Ponce DE León I Schmelz EA Gaggero C Castro A Alvarez A Montesano M 《Molecular Plant Pathology》2012,13(8):960-974
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Annelies Delporte Nausicaä Lannoo Gianni Vandenborre Maté Ongenaert Els J.M. Van Damme 《Plant Physiology and Biochemistry》2011,49(8):843-851
NICTABA is a carbohydrate-binding protein (also called lectin) that is expressed in several Nicotiana species after treatment with jasmonates and insect herbivory. Analyses with tobacco lines overexpressing the NICTABA gene as well as lines with reduced lectin expression have shown the entomotoxic effect of NICTABA against Lepidopteran larvae, suggesting a role of the lectin in plant defense. Until now, little is known with respect to the upstream regulatory mechanisms that are controlling the expression of inducible plant lectins. Using Arabidopsis thaliana plants stably expressing a promoter-β-glucuronidase (GUS) fusion construct, it was shown that jasmonate treatment influenced the NICTABA promoter activity. A strong GUS staining pattern was detected in very young tissues (the apical and root meristems, the cotyledons and the first true leaves), but the promoter activity decreased when plants were getting older. NICTABA was also expressed at low concentrations in tobacco roots and expression levels increased after cold treatment. The data presented confirm a jasmonate-dependent response of the promoter sequence of the tobacco lectin gene in Arabidopsis. These new jasmonate-responsive tobacco promoter sequences can be used as new tools in the study of jasmonate signalling related to plant development and defense. 相似文献
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Yan Y Stolz S Chételat A Reymond P Pagni M Dubugnon L Farmer EE 《The Plant cell》2007,19(8):2470-2483
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Jasmonates are naturally occurring signal compounds that regulate plant growth and development, and are involved in plant responses to several environmental stress factors. The mode of action of jasmonates has been investigated traditionally by analysis of the effects of exogenous application of these compounds, including identification of jasmonate-responsive genes and determination of their expression and responsive promoter elements. In addition, jasmonate biosynthesis has been studied by identification of biosynthetic enzymes, use of inhibitors and determination of endogenous jasmonate levels. Recently, several mutants defective in jasmonate biosynthesis and signaling have been isolated and their phenotypes shed new light on the role of jasmonates and jasmonate signaling in plant responses to pathogens, insects and ozone. 相似文献