现代分离技术在抗生素提取中的应用*

详细介绍了膜分离技术在抗生素分离提取中的应用进展,并简单阐明了高效毛细管电泳技术、双水相技术和反胶束萃取技术在抗生素提取中的应用情况。对这些现代分离技术的发展前景作了简要探讨。     

双水相技术在抗生素分离中的应用

简单阐明了双水相技术在抗生素的分离中应用的发展状况,分析了影响双水相系统分离抗生素的各种因素,提出了导致双水相中抗生素非对称分配的主要作用力为疏不作用力,并对双水相技术在抗生素分离中应用的发展方向作了探讨。     

双水相技术在抗生素分离中的应用

简单阐明了双水相技术在抗生素的分离中应用的发展状况,分析了影响双水相系统分离抗生素的各种因素,提出了导致双水相中抗生素非对称分配的主要作用力为疏水作用力,并对双水相技术在抗生素分离中应用的发展方向作了探讨。     

浅议膜分离技术在抗生素提取中的应用

抗生素在药物领域有着重要的作用,提取生产抗生素是药物生产的重要组成部分。因此,通过科学的方式来提取抗生素是药物科学领域的重要步骤。本文概述了通过膜分离技术来提取抗生素,而这里的膜分离技术主要包括纳滤膜、分渗透膜等多种膜分离技术,更好地为我国膜分离技术在抗生素提取上的应用发展出谋划策。     

现代生物工程分离提取技术在海洋生物资源开发上的应用

本文综述了在海洋资源开发上应用的现代生物工程分离技术。介绍了最新的分离提取技术如制备型和生产型色谱技术、超临界萃取技术、膜分离技术、毛细管电泳技术、酶工程技术等。从海洋生物资源中提取有用的生物活性物质,需要符合特定要求的现代生物工程技术。其中柱层析技术和超临界萃取技术是海洋生物活性物质分离提取技术中的主要部分。     

现代生物工程分离提取技术在海洋生物资源开发上的应用

本文综述了在海洋资源开发上应用的现代生物工程分离技术。介绍了最新的分离提取技术如制备型和生产型色谱技术、超临界萃取技术、膜分离技术、毛细管电泳技术、酶工程技术等。从海洋生物资源中提取有用的生物活性物质,需要符合特定要求的现代生物工程技术。其中柱层析技术和超临界萃取技术是海洋生物活性物质分离提取技术中的主要部分。     

试论提取分离纯化技术于制药过程的应用

当前社会飞速发展,科学技术取得了显著的发展成果,并且很好地应用于大众民生。医学临床诊疗过程中,需要用到先进的药物提纯技术,这就是对于医学科技的很好应用。本文将结合现实情况之下提取分离纯化技术在制药过程中的应用,更好地探讨分析整一个的技术应用,阐述其价值。结合蛋白质的提取分离纯化技术,来更好地分析其在制药过程中的应用。     

现代提取分离技术在食药用菌多糖分离纯化中的应用

详细综述了超声波法,酶解法,超滤法,透析法,色谱法等现代提取分离技术在食药用菌多糖分离纯化中的应用,简单阐述了各种技术的优缺点,并对其应用前景作了简要探讨。     

抗生素分离纯化技术研究进展

抗生素分离纯化是抗生素产业中的技术难点和关键环节,对抗生素常用的分离纯化技术及其研究进展进行了概述,以期为抗生素分离领域提供理论指导。最后对新型分离纯化技术进行了总结和展望。     

华东理工大学发酵工业分离提取技术研发中心

<正>简介华东理工大学发酵工业分离提取技术研发中心成立于2010年,是由华东理工大学和中国生物发酵产业协会共建的全国食品发酵行业专项技术中心。中心专业从事轻化工领域的分离提取关键技术、共性技术、技术集成研究,以及装备、介质研制和工程应用,广泛应用于抗生素、有机酸、氨基酸、酶制剂、疫苗及抗     

Separation of lactic acid from fermented broth by reactive extraction

The separation of lactic acid from complex fermentation broth was examined. Liquid–liquid extraction using reversible chemical complexation for reactive extraction was chosen to be the separation method. Over 50% yield of lactic acid was obtained from fermented broth in a single extraction step, when using the tertiary amine as the extractant, 1-dekanol as the diluent and trimethylamine (TMA) as the stripping solution. The effect of complex media on the extraction behaviour has hardly been examined previously.     

Biphasic recognition chiral extraction: A novel method for separation of mandelic acid enantiomers

This article reports a new chiral separation method-biphasic recognition chiral extraction for the separation of mandelic acid enantiomers. Distribution behavior of mandelic acid enantiomers was studied in the extraction system with O,O'-di-benzoyl-(2S,3S)-4-toluoyl-tartaric acid (D-(+)-DTTA) in organic phase and beta-CD derivatives in aqueous phase, and the influence of the types and concentrations of extractants and pH on extraction efficiency was investigated. Hydroxypropyl-beta-cyclodextrin (HP-beta-CD), hydroxyethyl-beta-cyclodextrin (HE-beta-CD), and methyl-beta-cyclodextrin (Me-beta-CD) have stronger recognition abilities for S-mandelic acid than those for R-mandelic acid, among which HP-beta-CD has the strongest ability. D-(+)-DTTA preferentially recognizes R-mandelic acid. pH and the concentrations of extractants have great effects on chiral separation ability. A high enantioseparation efficiency with a maximum enantioselectivity of 1.527 is obtained at pH of 2.7 and the ratio of 2:1 of [D-(+)-DTTA] to [HP-beta-CD]. The obtained results indicate that the biphasic recognition chiral extraction is of stronger chiral separation ability than the monophasic recognition chiral extraction. It may be very helpful to optimize the extraction systems and realize the large-scale production of pure enantiomers.     

Enantioseparation of Racemic Flurbiprofen by Aqueous Two‐Phase Extraction With Binary Chiral Selectors of L‐dioctyl Tartrate and L‐tryptophan

A novel method for chiral separation of flurbiprofen enantiomers was developed using aqueous two‐phase extraction (ATPE) coupled with biphasic recognition chiral extraction (BRCE). An aqueous two‐phase system (ATPS) was used as an extracting solvent which was composed of ethanol (35.0% w/w) and ammonium sulfate (18.0% w/w). The chiral selectors in ATPS for BRCE consideration were L‐dioctyl tartrate and L‐tryptophan, which were screened from amino acids, β‐cyclodextrin derivatives, and L‐tartrate esters. Factors such as the amounts of L‐dioctyl tartrate and L‐tryptophan, pH, flurbiprofen concentration, and the operation temperature were investigated in terms of chiral separation of flurbiprofen enantiomers. The optimum conditions were as follows: L‐dioctyl tartrate, 80 mg; L‐tryptophan, 40 mg; pH, 4.0; flurbiprofen concentration, 0.10 mmol/L; and temperature, 25 °C. The maximum separation factor α for flurbiprofen enantiomers could reach 2.34. The mechanism of chiral separation of flurbiprofen enantiomers is discussed and studied. The results showed that synergistic extraction has been established by L‐dioctyl tartrate and L‐tryptophan, which enantioselectively recognized R‐ and S‐enantiomers in top and bottom phases, respectively. Compared to conventional liquid–liquid extraction, ATPE coupled with BRCE possessed higher separation efficiency and enantioselectivity without the use of any other organic solvents. The proposed method is a potential and powerful alternative to conventional extraction for separation of various enantiomers. Chirality 27:650–657, 2015. © 2015 Wiley Periodicals, Inc.     

Fast analysis using monolithic columns coupled with high-flow on-line extraction and electrospray mass spectrometric detection for the direct and simultaneous quantitation of multiple components in plasma

In this work, monolithic columns were used as a fast separation tool for multiple-component quantitative liquid chromatography-tandem mass spectrometry (LC-MS-MS) assays of drug candidates in biological fluids. A considerably reduced runtime was achieved while maintaining good chromatographic separations. This significantly improved separation speed demanded higher throughput on sample extraction. To this end, monolithic separations were coupled on-line with high-flow extraction, which allowed for the fast extraction and separation of samples containing multiple analytes. An evaluation of this system was performed using a mixture of fenfluramine, temazepam, oxazepam, and tamoxifen in plasma. A total cycle time of 1.2 min was achieved which included both sample extraction and subsequent monolithic column separation via column switching. A total of over 400 plasma samples were analyzed in less than 10 h. The sensitivity and responses were reproducible throughout the run. The system has been routinely used in the authors' laboratory for high-throughput quantitation of compounds in biological fluids in support of drug discovery programs. The assay for samples from a 9-in-1 dog pharmacokinetic study is shown as an example to demonstrate the capability of this system.     

我国生物丁醇分离提取技术研究进展

本文概述了目前国内外生物丁醇生产体系中的分离提取技术,包括液液萃取、气提、吸附、精馏和渗透汽化技术等的应用研究现状,详细阐述了上述几种方法在分离提取生物丁醇方面的优势与不足,对各类方法的分离特性和效果进行了比较,并展望了生物丁醇分离提取技术的发展前景。     

用超临界流体技术萃取分离香茅油的研究

作者用超临界流体循环萃取装置对香茅油在超临界CO_2中的溶解度以及香茅油中主要成份香茅醛、香茅醇和香叶醇的分离特性进行研究。分别得出溶解度、分离特性与萃取压力、温度的关系及其相应的数学关联式。用这些关联式计算的结果符合工程需要,为单离香茅油工业化生产提供了理论基础。     

超临界CO2萃取红景天中红景天苷、苷元酪醇的研究

采用超临界CO₂ 萃取法和乙醇常温浸提法相比较, 研究从红景天中提取红景天苷、苷元酪醇的工艺条件, 结论是:采用超临界CO₂ 萃取法能萃取出红景天生药中红景天苷的1.2%, 提取率不高, 但该方法能萃取出80%的苷元酪醇, 萃取液中苷元酪醇的相对含量可达45.68%;乙醇常温浸提法能将红景天苷、苷元酪醇同时有效萃取, 且得率较高, 但是萃取液中两物质相对含量较低, 进一步分离纯化将有难度。本研究结果表明, 将超临界CO₂ 萃取法和乙醇常温浸提法有效结合, 可实现两物质的有效分离, 推进红景天有效成分的产业化进程。     

Fast, fully automated analysis of voriconazole from serum by LC-LC-ESI-MS-MS with parallel column-switching technique

Voriconazole is a novel broad-spectrum antifungal agent. We developed an on-line LC-LC-MS-MS method for fully automated and direct analysis of voriconazole in raw human serum. After injection of human serum size-selective sample fractionation and analyte extraction was achieved using an extraction column (25 mm x 4 mm) packed with a restricted access material (RAM, LiChrospher ADS C(8), 25 microm). On-line transfer of voriconazole from the extraction column was followed by chromatography separation on a C(18) column. Detection was done by ESI-MS-MS. The total analysis time was 13 min, managed by parallel extraction and chromatographic separation. This LC-MS assay was fully validated. The lower limit of quantification was 0.05 microg/ml. The automated inline extraction of voriconazole described here eliminates the need for difficult and time-consuming sample pre-treatment. Other advantages of the new method are that only a small quantity (5 microl) of serum is needed and that the method is very specific.     

Selective separation of amino acids by reactive extraction

The method of reactive extraction with di-(2-ethylhexyl)phosphoric acid (D2EHPA) for the separation of a range of amino acids is studied. The results obtained on the individual reactive extraction indicated the possibility of the amino acids selective separation as a function of the pH value of aqueous solution and the acidic or basic character of each amino acid. Thus, using multistage extraction, the total separation of the following amino acids groups has been performed: neutral amino acids (l-glycine, l-alanine, l-tryptophan) at pH 5–5.5 (nine extraction stages), basic amino acids (l-lysine, l-arginine) and l-cysteine at pH 4–4.5 (ten extraction stages), l-histidine at pH 3–3.5 (five extraction stages), and acidic amino acids (l-aspartic acid, l-glutamic acid) at pH 2–2.5 (three extraction stages).