共查询到20条相似文献,搜索用时 46 毫秒
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Harrod R Kuo YL Tang Y Yao Y Vassilev A Nakatani Y Giam CZ 《The Journal of biological chemistry》2000,275(16):11852-11857
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Polesskaya A Duquet A Naguibneva I Weise C Vervisch A Bengal E Hucho F Robin P Harel-Bellan A 《The Journal of biological chemistry》2000,275(44):34359-34364
The myogenic protein MyoD requires two nuclear histone acetyltransferases, CREB-binding protein (CBP)/p300 and PCAF, to transactivate muscle promoters. MyoD is acetylated by PCAF in vitro, which seems to increase its affinity for DNA. We here show that MyoD is constitutively acetylated in muscle cells. In vitro, MyoD is acetylated both by CBP/p300 and by PCAF on two lysines located at the boundary of the DNA binding domain. MyoD acetylation by CBP/p300 (as well as by PCAF) increases its activity on a muscle-specific promoter, as assessed by microinjection experiments. MyoD mutants that cannot be acetylated in vitro are not activated in the functional assay. Our results provide direct evidence that MyoD acetylation functionally activates the protein and show that both PCAF and CBP/p300 are candidate enzymes for MyoD acetylation in vivo. 相似文献
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Micol Tillhon Ornella Cazzalini Tiziana Nardo Daniela Necchi Sabrina Sommatis Lucia A. Stivala A. Ivana Scovassi Ennio Prosperi 《DNA Repair》2012,11(10):844-852
Nucleotide excision repair (NER) is an important DNA repair mechanism through which cells remove bulky DNA lesions. Following DNA damage, the histone acetyltransferase (HAT) p300 (also referred to as lysine acetyltransferase or KAT) is known to associate with proliferating cell nuclear antigen (PCNA), a master regulator of DNA replication and repair processes. This interaction, which results in HAT inhibition, may be dissociated by the cell cycle inhibitor p21CDKN1A, thereby restoring p300 activity; however, the role of this protein interplay is still unclear. Here, we report that silencing p300 or its homolog CREB-binding protein (CBP) by RNA interference (RNAi) significantly reduces DNA repair synthesis in human fibroblasts. In addition, we determined whether p300 and CBP may associate with and acetylate specific NER factors such as XPG, the 3′-endonuclease that is involved in the incision/excision step and is known to interact with PCNA. Our results show that p300 and CBP interact with XPG, which has been found to be acetylated in vivo. XPG is acetylated by p300 in vitro, and this reaction is inhibited by PCNA. Knocking down both p300/CBP by RNAi or by chemical inhibition with curcumin greatly reduced XPG acetylation, and a concomitant accumulation of the protein at DNA damage sites was observed. The ability of p21 to bind PCNA was found to regulate the interaction between p300 and XPG, and an abnormal accumulation of XPG at DNA damage sites was also found in p21−/− fibroblasts. These results indicate an additional function of p300/CBP in NER through the acetylation of XPG protein in a PCNA–p21 dependent manner. 相似文献
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Histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein 下载免费PDF全文
Guidez F Howell L Isalan M Cebrat M Alani RM Ivins S Hormaeche I McConnell MJ Pierce S Cole PA Licht J Zelent A 《Molecular and cellular biology》2005,25(13):5552-5566
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In vivo chromatin remodeling events leading to inflammatory gene transcription under diabetic conditions 总被引:5,自引:0,他引:5
Miao F Gonzalo IG Lanting L Natarajan R 《The Journal of biological chemistry》2004,279(17):18091-18097
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Interaction and functional cooperation between the LIM protein FHL2, CBP/p300, and beta-catenin 总被引:1,自引:0,他引:1 下载免费PDF全文
Labalette C Renard CA Neuveut C Buendia MA Wei Y 《Molecular and cellular biology》2004,24(24):10689-10702
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