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Spatial analysis of lipid metabolites and expressed genes reveals tissue‐specific heterogeneity of lipid metabolism in high‐ and low‐oil Brassica napus L. seeds 下载免费PDF全文
Shaoping Lu Drew Sturtevant Mina Aziz Cheng Jin Qing Li Kent D. Chapman Liang Guo 《The Plant journal : for cell and molecular biology》2018,94(6):915-932
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Members of the Pinaceae family have complex chemical defense strategies. Conifer defenses associated with specialized cell types of the bark involve constitutive and inducible accumulation of phenolic compounds in polyphenolic phloem parenchyma cells and oleoresin terpenoids in resin ducts. These defenses can protect trees against insect herbivory and fungal colonization. The phytohormone ethylene has been shown to induce the same anatomical and cellular defense responses that occur following insect feeding, mechanical wounding, or fungal inoculation in Douglas fir (Pseudotsuga menziesii) stems (Hudgins and Franceschi in Plant Physiol 135:2134–2149, 2004). However, very little is known about the genes involved in ethylene formation in conifer defense or about the temporal and spatial patterns of their protein expression. The enzyme 1-aminocyclopropane-1-carboxylate oxidase (ACO) catalyzes the final step in ethylene biosynthesis. We cloned full-length and near full-length ACO cDNAs from three conifer species, Sitka spruce (Picea sitchensis), white spruce (P. glauca), and Douglas fir, each with high similarity to Arabidopsis thaliana ACO proteins. Using an Arabidopsis anti-ACO antibody we determined that ACO is constitutively expressed in Douglas fir stem tissues and is up-regulated by mechanical wounding, consistent with the wound-induced increase of ethylene levels. Immunolocalization showed cytosolic ACO is predominantly present in specialized cell types of the wound-induced bark, specifically in epithelial cells of terpenoid-producing cortical resin ducts, in polyphenolic phloem parenchyma cells, and in ray parenchyma cells.J.W. Hudgins and Steven G. Ralph contributed equally to this work. 相似文献
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Pierre‐François Perroud Fabian B. Haas Manuel Hiss Kristian K. Ullrich Alessandro Alboresi Mojgan Amirebrahimi Kerrie Barry Roberto Bassi Sandrine Bonhomme Haodong Chen Juliet C. Coates Tomomichi Fujita Anouchka Guyon‐Debast Daniel Lang Junyan Lin Anna Lipzen Fabien Nogué Melvin J. Oliver Inés Ponce de León Ralph S. Quatrano Catherine Rameau Bernd Reiss Ralf Reski Mariana Ricca Younousse Saidi Ning Sun Péter Szövényi Avinash Sreedasyam Jane Grimwood Gary Stacey Jeremy Schmutz Stefan A. Rensing 《The Plant journal : for cell and molecular biology》2018,95(1):168-182
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Maria Mar Marquès‐Bueno Ana K. Morao Anne Cayrel Matthieu P. Platre Marie Barberon Erwann Caillieux Vincent Colot Yvon Jaillais François Roudier Grégory Vert 《The Plant journal : for cell and molecular biology》2016,85(2):320-333
Multicellular organisms are composed of many cell types that acquire their specific fate through a precisely controlled pattern of gene expression in time and space dictated in part by cell type‐specific promoter activity. Understanding the contribution of highly specialized cell types in the development of a whole organism requires the ability to isolate or analyze different cell types separately. We have characterized and validated a large collection of root cell type‐specific promoters and have generated cell type‐specific marker lines. These benchmarked promoters can be readily used to evaluate cell type‐specific complementation of mutant phenotypes, or to knockdown gene expression using targeted expression of artificial miRNA. We also generated vectors and characterized transgenic lines for cell type‐specific induction of gene expression and cell type‐specific isolation of nuclei for RNA and chromatin profiling. Vectors and seeds from transgenic Arabidopsis plants will be freely available, and will promote rapid progress in cell type‐specific functional genomics. We demonstrate the power of this promoter set for analysis of complex biological processes by investigating the contribution of root cell types in the IRT1‐dependent root iron uptake. Our findings revealed the complex spatial expression pattern of IRT1 in both root epidermis and phloem companion cells and the requirement for IRT1 to be expressed in both cell types for proper iron homeostasis. 相似文献
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Transposon‐driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution 下载免费PDF全文
Harpreet K Saini Stijn van Dongen Tommaso Leonardi Giovanni Bussotti Jack M Monahan Tania Auchynnikava Angelo Bitetti Juri Rappsilber Robin C Allshire Alena Shkumatava Dónal O'Carroll Anton J Enright 《EMBO reports》2017,18(7):1231-1247
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Phytochromes are red‐ and far red light photoreceptors in higher plants. Rice (Oryza sativa L.) has three phytochromes (phyA, phyB and phyC), which play distinct as well as cooperative roles in light perception. To gain a better understanding of individual phytochrome functions in rice, expression patterns of three phytochrome genes were characterized using promoter‐GUS fusion constructs. The phytochrome genes PHYA and PHYB showed distinct patterns of tissue‐ and developmental stage‐specific expression in rice. The PHYA promoter‐GUS was expressed in all leaf tissues in etiolated seedlings, while its expression was restricted to vascular bundles in expanded leaves of light‐grown seedlings. These observations suggest that light represses the expression of the PHYA gene in all cells except vascular bundle cells in rice seedlings. Red light was effective, but far red light was ineffective in gene repression, and red light‐induced repression was not observed in phyB mutants. These results indicate that phyB is involved in light‐dependent and tissue‐specific repression of the PHYA gene in rice. 相似文献