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Phalaenopsisisagenusofsome 4 0speciesmainlydistributedinthePhilippinesandIndonesia ,withsomerepresenta tivesextendingtomainlandAsiaandAustralia .SixofthemarefoundinChina .InthepresentpapertheseventhChinesespecies,P malipoensisZ .J .LiuetS .C .Chen ,isdesc… 相似文献
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蝴蝶兰的组织培养研究 总被引:17,自引:2,他引:15
探讨蝴蝶兰的组织培养技术和方法。实验表明:改良型M1培养基诱导原球茎有良好的效果,M1 BA5.0mg/L对花梗芽的诱导最合适,Ml BA3.0mg/L对茎尖诱导原球茎最合适。降低分裂素浓度后,原球茎可以分化成完整植株,壮苗阶段以改良型M2 BA0.1mg/L NAA0.5mg/L效果较佳。移栽基质选用水苔,成活率高。 相似文献
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近几年,蝴蝶兰越来越多地出现在各地的花展和花市上。她的花朵大、色彩绚丽、花姿优雅,花期长达l-2个月,素有“兰花皇后”的美誉。蝴蝶兰(PhaL比"opshspp)属兰科植物,是“洋兰”的一个类群。其叶互生,表面有蜡质光泽。花茎修长,花朵形如蝴坡,故而得名。人工栽培蝴蝶兰已有一百多年的历史,但由于蝴蝶兰属单茎性兰,只有一个生长点,又不易通过分株来繁殖,在自然条件下,种子难发芽,所以蝴蝶兰显得弥足珍贵。进入本世纪80年代末,蝴蝶兰可以通过生物技术进行大量复制,可以生产出众多性状一致的幼苗,使得工厂化生产蝴蝶兰成为… 相似文献
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Phalaenopsis malipoensis Z.J.Liu et S.C.Chen,a new species of Orchidaceae from southeastern Yunnan,is described and illustrated.This is a quite distinct species from those known from China and its adjacent regions.It shows a faint resemblance to Phalaenopsis gibbosa Sweet of Laos and Vietnam,but differs by having narrower petals,not zigzag rachis and a large callus on the mid lobe of the lip which is deeply forked with each arm dividing into 2 filiform linear antennae. 相似文献
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蝴蝶兰原球茎液体增殖培养的研究 总被引:1,自引:0,他引:1
以茎尖诱导形成的原球茎为外殖体,采用液体培养方式探讨了不同浓度的激素配比、蔗糖浓度和添加不同量的新鲜液汁对蝴蝶兰原球茎增殖的影响,并比较了液体和固体两种培养方式的原球茎增殖特征.结果表明:不同浓度的外源激素及其配比对蝴蝶兰原球茎增殖影响不明显,但对原球茎生长影响较大,6-BA 2.0 mg/L+Ad 1.0 mg/L+NAA 0.5 mg/L的激素浓度组合比较适合蝴蝶兰原球茎增殖,蔗糖浓度对蝴蝶兰原球茎增殖和生长影响较大,适合蝴蝶兰PLBs增殖的蔗糖浓度为20 g/L,添加10 % ~40 %的新鲜椰汁对蝴蝶兰原球茎增殖生长有明显促进作用;原球茎液体增殖培养的时间不宜超过4周,5周内连续培养增殖曲线呈倒"V"字形;液体增殖培养的原球茎分化成苗的时间相对较晚. 相似文献
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蝴蝶兰EST-SSRs分析 总被引:4,自引:0,他引:4
对蝴蝶兰属EST序列进行了SSR分析。蝴蝶兰属EST总长为4.5Mb,含有609个SSR。SSR出现频率7.65%,平均距离7.39kb,平均长度为22.17bp。单碱基、二碱基和三碱基重复是主要重复类型,分别占EST-SSR总数的21.67%、40.39%和33.50%。A、AG和CCG分别是单碱基、二碱基和三碱基重复中主导重复基元,分别占96.21%、58.54%和32.25%。设计引物及检测的结果表明,蝴蝶兰EST-SSR标记对兰科其他属植物具有一定的通用性。 相似文献
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In the attempt to discover new genes involved in the floral development in monoeotyledonousin species,we have cloned and characterized the homologous PISTALLATA-like (PI-like) gone from Phalaenopsis hybrid cultivar named PhPI9 (Phalaenopsis PI STILLATA # 9).The eDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis.The deduced amino acid sequence of PhPI9 had the typical PI-motif.It also formed a subelade with other monoeot PI-type genes in phylogenetie analysis.Southern analysis showed that PhPI9 was present in the Phalaenopsis orchid genome as a single copy.Furthermore,it was expressed only in the lip of the Phalaenopsis flower and no expression was detected in vegetative organs.Thus,as a B-function MADS-box gone,PhP19 specifies floral organ identity in orchids. 相似文献
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针对蝴蝶兰叶片蛋白质含量少且含有大量色素和酚等干扰物质的特点,通过对总蛋白提取方法、银染方法的改进,以及双向电泳实验条件的比较选择,初步建立一套适用于蝴蝶兰叶片蛋白质组分析的双向电泳技术。 相似文献
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Phylogenetic trees inferred from the internal transcribed spacers 1 and 2 (ITS1 + ITS2) region from nuclear ribosomal DNA (nrDNA) and the intergenic spacer of atpB-rbcL from chloroplast DNA (cpDNA) were used to clarify the phylogenetics and evolutionary trends of the Phalaenopsis sumatrana complex. The P. sumatrana complex includes the two species P. sumatrana and Phalaenopsis corningiana as well as a problem species, Phalaenopsis zebrina, according to the concepts of Sweet (1980) and Christenson (2001). Based on the phylogenetic tree inferred from the ITS sequence, the accessions of P. sumatrana cannot be separated from those of P. corningiana. In contrast, the accessions of P. zebrina can be separated from those of both P. sumatrana and P. corningiana. However, analyses of the sequences of the atpB-rbcL spacer apparently cannot discriminate among these three species of the P. sumatrana complex. An inspection of the morphological characters of the plants of the P. sumatrana complex and the floral fragrances of P. zebrina can be used to separate it from both P. sumatrana and P. corningiana. Based on the molecular data and floral fragrances, P. zebrina perhaps should not be treated as a synonym of P. sumatrana. On the evolutionary trend of the P. sumatrana complex, P. zebrina was suggested to be the relative origin group of the P. sumatrana complex based on the phylogenetic tree and biogeography. 相似文献
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研究了蝴蝶兰(Phalaenopsis sp.)叶片外植体褐变过程中PAL基因表达的变化。结果表明,在整个褐变过程中,外植体的PAL基因表达出现差异,离体培养第3天的表达明显提高,一直到第8天还维持较高表达水平,以后随着外植体褐变的加重,PAL基因表达水平逐渐降低。与对照相比,在Fe盐浓度加倍为55.6 mg L-1培养基中培养的外植体PAL基因表达水平提高发生的时间比对照早,培养第2天就明显增强,随培养天数的延长,一直维持较高的表达水平;其PAL活性也高于对照,两种培养条件下,外植体总酚含量都随着其褐变加重而增加,说明PAL基因表达与蝴蝶兰外植体褐变过程相关。 相似文献