共查询到20条相似文献,搜索用时 15 毫秒
1.
《Molecular & cellular proteomics : MCP》2019,18(6):1085-1095
Highlights
- •Rapamycin and zinc induce moderate but significant mitochondrial proteome changes.
- •The mitochondrial proteins processing system is robust under subtoxic conditions.
- •Rapamycin and zinc perturb the mitochondrial proteins processing system.
- •Rapamycin and zinc perturb the mitochondrial proteins homeostasis.
2.
《Molecular & cellular proteomics : MCP》2018,17(11):2119-2131
Highlights
- •Temporal proteome profiling of lipotoxicity and glucolipotoxicity in β-cells
- •Palmitate induced cholesterol metabolism earlier than fatty acid metabolism
- •Setd8 promotes palmitate + glucose-stimulated INS-1 cell proliferation
- •PA induced apoptosis partially via upregulation of Rhob in INS-1 cells
3.
《Molecular & cellular proteomics : MCP》2019,18(6):1036-1053
Highlights
- •Establishment of a flow system allowing multi-omics analysis of S. aureus biofilms.
- •Biofilm proteome profiling (intracellular and ECM) plus metabolic footprint analysis.
- •Virulence factors and ribosomal proteins stabilize the ECM as moonlighting proteins.
- •They act as electrostatic bridges between anionic cell surfaces, eDNA and metabolites.
4.
Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*
《Molecular & cellular proteomics : MCP》2019,18(5):909-922
Highlights
- •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
- •Label-free quantification determined 2843 phagosomal proteins.
- •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
- •Increased recruitment of antigen cross-presentation molecules to these phagosomes.
5.
《Molecular & cellular proteomics : MCP》2019,18(7):1410-1427
Highlights
- •Quantitative (phoshpo)proteome of primary cell cultures of patient-matched prostate CAF and NPF.
- •Key CAF-associated proteins validated using orthogonal methodologies.
- •LOXL2 inhibitors D-penicillamine and PXS-S2A impaired CAF migration and ECM alignment.
- •Pre-treatment with LOXL2 inhibitors impaired migratory capacity of RWPE-2 cells in co-culture.
6.
《Molecular & cellular proteomics : MCP》2019,18(9):1705-1720
Highlights
- •Quantitative changes in global proteome and ubiquitinome in Huntington's disease.
- •Differential ubiquitination of wild-type and mutant Htt in mice brain.
- •Enriched pathways include vesicle transport and mRNA processing.
- •Correlation between protein and diGly site fold changes.
7.
《Molecular & cellular proteomics : MCP》2018,17(12):2496-2507
Highlights
- •Quantitative (phospho)proteome analysis of antibiotic treatment in E. coli.
- •Largest bacterial phosphorylation catalogue.
- •Specific phosphorylation motifs changes during resistance development.
- •Phosphorylation mediated signaling could be a potential target for drug design.
8.
《Molecular & cellular proteomics : MCP》2019,18(6):1123-1137
Highlights
- •Changes to the proteome of skin fibroblasts subjected to reductive stress have been quantitated.
- •Only a small set of proteins is selectively diminished upon exposure to reductants.
- •Collagens (COL1A2 and COL6A2) emerge as sentinels of reductive stress.
- •Reductive stress triggers receptor-independent Akt phosphorylation at Ser473.
9.
《Molecular & cellular proteomics : MCP》2019,18(8):1511-1525
Highlights
- •First global study on laryngeal cells cultured with cigarette smoke enriched medium.
- •Vocal fold fibroblasts increased production of ECM component hyaluronan.
- •Expression of several fibrillar collagens was reduced.
10.
《Molecular & cellular proteomics : MCP》2019,18(5):936-953
Highlights
- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
11.
《Molecular & cellular proteomics : MCP》2019,18(4):606-621
Highlights
- •In-depth proteome underpins the gland ontogeny and age-specific activity of the HGs.
- •The well-developed acini in the HGs of NBs promote the RJ secretary activities.
- •The enhanced protein and energy metabolism in the HGs boost the stronger RJ secretion of RJBs.
12.
《Molecular & cellular proteomics : MCP》2019,18(9):1864-1879
Highlights
- •Feeding mice a western-style obesogenic diet resulted in dramatic changes in fecal microbial proteome.
- •Basal fecal microbial proteome, but not microbiome, composition was associated with extent of diet-induced obesity.
- •A major feature of fecal microbial proteome of high-responder mice was enriched motility-related proteins.
13.
《Molecular & cellular proteomics : MCP》2018,17(12):2518-2533
Highlights
- •Chromobodies are stabilized by antigen binding in live cells.
- •Monitoring changes of endogenous protein levels in living cells with chromobodies.
- •Broadly applicable system to generate turnover-accelerated chromobodies.
- •Quantification of time- and dose-dependent compound effects.
14.
《Molecular & cellular proteomics : MCP》2019,18(12):2506-2515
Highlights
- •Method for the analysis of response curves from thermal proteome profiling (TPP).
- •NPARC uses nonparametric statistics and provides false discovery-rate (FDR) control.
- •Increased proteome coverage and sensitivity to identify drug-binding proteins.
15.
《Molecular & cellular proteomics : MCP》2019,18(11):2138-2148
Highlights
- •Highly glycosylated matrisome proteins and their binding partners comprise extracellular networks that mediate tissue-specific cellular microenvironments.
- •Elucidation of roles of matrisome molecules in disease mechanisms requires detailed mapping of matrisome glycosylation and other post-translational modifications.
- •We review tissue workup methods for matrisome proteomics, glycomics and glycoproteomics.
- •The combination of proteomics, glycomics and glycoproteomics profiles matrisome protein modifications distinct from those studied by immunohistochemistry.
16.
《Molecular & cellular proteomics : MCP》2019,18(9):1899-1915
Highlights
- •Development of a method for low-abundance small protein analysis in human plasma.
- •Deep proteome profiling demonstrated unbiased identification of diverse factors.
- •Method enabled identification of a novel human plasma protein C5ORF46.
- •Analysis of intermittent fasting response showed changes in iron regulation.
17.
《Molecular & cellular proteomics : MCP》2019,18(10):2108-2120
Highlights
- •Bayesian Beta-Binomial model integrates ion statistics with peptide ratio agreement.
- •Model appropriately interprets information from low signal peptides.
- •Confidence can be assigned even without replicates.
- •Model adds sensitivity to detection of small changes.
18.
19.
《Molecular & cellular proteomics : MCP》2019,18(6):1110-1122
Highlights
- •Comprehensive analysis of inter-individual variation of normal urinary proteome.
- •Significant gender differences were observed.
- •Proteins increased in female urine are enriched in immunological pathways.
- •Estimated reference intervals of proteins as the baseline for biomarker discovery.
20.
《Molecular & cellular proteomics : MCP》2018,17(11):2146-2163
Highlights
- •Construction of threespine stickleback gill assay library using DDA proteomics
- •Population-specific gill proteome signatures of four ecotypes identified by DIA
- •HSP47 and extracellular matrix proteins highly elevated in warm-adapted sticklebacks
- •Inflammasome and proteolytic proteins highly elevated in freshwater sticklebacks