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1.
《Molecular & cellular proteomics : MCP》2019,18(5):968-981
Highlights
- •Quantitative substrate profiling method for characterizing peptidase specificity.
- •Applicable to both purified peptidases and peptidases in complex biological samples.
- •TMT labeling improves throughput, accuracy and reproducibility of the assay.
- •Design of fluorescent probes to monitor peptidase activity based on substrate data.
2.
《Molecular & cellular proteomics : MCP》2019,18(6):1183-1196
Highlights
- •Atlantic salmon O-glycome expanded to 169 structures in three epithelia.
- •Low interindividual variation amongst all populations and geographical regions.
- •Small variations in glycosylation between geographical locations and fish size.
- •Prominent fucosylation in gastrointestinal mucins from Tasmanian fish.
3.
《Molecular & cellular proteomics : MCP》2019,18(5):854-864
Highlights
- •Zero-length chemical cross-linking of APOA1 peptides in HDL.
- •Cross-links match antiparallel isomers of APOA dimers in molecular modeling.
- •Identical MS/MS spectra of native and synthetic cross-linked peptides.
- •First biochemical evidence of LL5/5 and LL5/4 isomers in human HDL.
4.
《Molecular & cellular proteomics : MCP》2019,18(6):1227-1241
Highlights
- •Quantitative microproteomics to study the CNS and PNS of the Twitcher mouse.
- •10plex TMT experiments on corpus callosum, motor cortex and sciatic nerves extracts.
- •More than 400 proteins groups deregulated between Twitcher and wildtype mice.
- •New insights into the molecular mechanisms of Krabbe disease.
5.
《Molecular & cellular proteomics : MCP》2019,18(7):1396-1409
Highlights
- •A panel of HEK293 isogenic cell lines with knockout of GALNT genes.
- •Identification of nonredundant O-glycosylation sites regulated by specific GalNAc-T isoforms.
- •GalNAc-T7 and T10 contribute to follow-up activity in regions of high density O-glycosylation.
- •GalNAc-T11 specifically controls O-glycosylation of specific linker regions in the low-density lipoprotein receptor related proteins.
6.
《Molecular & cellular proteomics : MCP》2019,18(3):594-605
Highlights
- •A new strategy for simultaneous quantification of protein expression and modification.
- •This top-down LC/MS-based method shows high reproducibility and high throughput.
- •Quantification at the intact protein level with results comparable to Western blot.
- •This top-down proteomics method is applicable to different species and tissues.
7.
《Molecular & cellular proteomics : MCP》2019,18(5):936-953
Highlights
- •In-depth proteome profiling of primary human myeloma cells
- •Characteristics of myeloma cells are related to hypoxic bone marrow conditions
- •Myeloma cells show specific immune evasion strategies
- •Metabolic adaptations involve tumor and stroma cells
8.
《Molecular & cellular proteomics : MCP》2019,18(5):876-891
Highlights
- •Three novel Conodipines P1-3 in the injected venom of Conus purpurascens.
- •Conodipines P1-3 have consensus catalytic characteristics of sPLA2.
- •We determined multiple modification sites in Conodipines P1-3.
- •Evaluated the activity of Conohyal-P1 by a MS-based method.
9.
《Molecular & cellular proteomics : MCP》2019,18(6):1110-1122
Highlights
- •Comprehensive analysis of inter-individual variation of normal urinary proteome.
- •Significant gender differences were observed.
- •Proteins increased in female urine are enriched in immunological pathways.
- •Estimated reference intervals of proteins as the baseline for biomarker discovery.
10.
《Molecular & cellular proteomics : MCP》2019,18(6):1171-1182
Highlights
- •First report on the quantitative proteomic profiling of Drosophila lymph glands.
- •Comparative proteomic analysis under conditions of perturbed blood cell homeostasis.
- •Resource for identifying new regulators of insect and vertebrate hematopoiesis.
11.
《Molecular & cellular proteomics : MCP》2019,18(10):2089-2098
Highlights
- •Cathepsin-L is introduced as a novel protease for HX-MS studies.
- •Cathepsin-L improves resolution of traditionally challenging histone tails.
- •Cathepsin-L can be readily combined with pepsin for improved protein coverage.
- •In-solution dynamics of the H3.1 and H4 monomers reveal extensive EX1 kinetics.
12.
《Molecular & cellular proteomics : MCP》2019,18(6):1085-1095
Highlights
- •Rapamycin and zinc induce moderate but significant mitochondrial proteome changes.
- •The mitochondrial proteins processing system is robust under subtoxic conditions.
- •Rapamycin and zinc perturb the mitochondrial proteins processing system.
- •Rapamycin and zinc perturb the mitochondrial proteins homeostasis.
13.
《Molecular & cellular proteomics : MCP》2019,18(8):1630-1650
Highlights
- •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
- •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
- •Druggability, outcomes, and immune signatures related to kinase-substrates.
- •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.
14.
Posttranslational Modifications Drive Protein Stability to Control the Dynamic Beer Brewing Proteome
《Molecular & cellular proteomics : MCP》2019,18(9):1721-1731
Highlights
- •SWATH-MS profiles protein abundance and modification during mashing in beer brewing.
- •Proteolysis due to barley proteases leads to extreme proteoform diversity.
- •Sequence-specific proteolytic clipping of proteins controls their stability.
15.
《Molecular & cellular proteomics : MCP》2019,18(3):461-476
Highlights
- •Genital samples were analyzed using a high-throughput bead-based affinity assay.
- •Proteins were validated by tandem mass spectrometry with concordant results.
- •Genital epithelial proteins were increased in HIV-serodiscordant women.
16.
《Molecular & cellular proteomics : MCP》2019,18(4):657-668
Highlights
- •Global proteomic remodeling alters antibiotic susceptibility in K. pneumoniae.
- •Drug specific transport, sugar utilization, central metabolism, capsule synthesis.
- •Common pathways of reactive oxygen scavenging, turnover of misfolded proteins.
- •Integrated adjustments and unique drug-specific features for drug combinations.
17.
18.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
Highlights
- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.
19.
《Molecular & cellular proteomics : MCP》2019,18(6):1157-1170
Highlights
- •Auxin responsive proteins in Arabidopsis roots were identified from 3,514 detected proteins.
- •All six auxin receptors are stable in response to hormone via novel MRM assays.
- •The >100 differentially expressed proteins exhibit dynamic and transient responses to auxin.
- •Phenotypic screening of the top responsive proteins uncovered several novel root mutants.
20.
Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*
《Molecular & cellular proteomics : MCP》2019,18(5):909-922
Highlights
- •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
- •Label-free quantification determined 2843 phagosomal proteins.
- •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
- •Increased recruitment of antigen cross-presentation molecules to these phagosomes.