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1.
Development and mapping of microsatellite (SSR) markers in wheat 总被引:46,自引:9,他引:37
Song QJ Shi JR Singh S Fickus EW Costa JM Lewis J Gill BS Ward R Cregan PB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(3):550-560
Microsatellite DNA markers are consistently found to be more informative than other classes of markers in hexaploid wheat. The objectives of this research were to develop new primers flanking wheat microsatellites and to position the associated loci on the wheat genome map by genetic linkage mapping in the ITMI W7984 × Opata85 recombinant inbred line (RIL) population and/or by physical mapping with cytogenetic stocks. We observed that the efficiency of marker development could be increased in wheat by creating libraries from sheared rather than enzyme-digested DNA fragments for microsatellite screening, by focusing on microsatellites with the [ATT/TAA]n motif, and by adding an untemplated G-C clamp to the 5-end of primers. A total of 540 microsatellite-flanking primer pairs were developed, tested, and annotated from random genomic libraries. Primer pairs and associated loci were assigned identifiers prefixed with BARC (the acronym for the USDA-ARS Beltsville Agricultural Research Center) or Xbarc, respectively. A subset of 315 primer sets was used to map 347 loci. One hundred and twenty-five loci were localized by physical mapping alone. Of the 222 loci mapped with the ITMI population, 126 were also physically mapped. Considering all mapped loci, 126, 125, and 96 mapped to the A, B, and D genomes, respectively. Twenty-three of the new loci were positioned in gaps larger than 10 cM in the map based on pre-existing markers, and 14 mapped to the ends of chromosomes. The length of the linkage map was extended by 80.7 cM. Map positions were consistent for 111 of the 126 loci positioned by both genetic and physical mapping. The majority of the 15 discrepancies between genetic and physical mapping involved chromosome group 5.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
2.
Xin Chen Zhiqiang Xia Yuhua Fu Cheng Lu Wenquan Wang 《Plant Molecular Biology Reporter》2010,28(4):676-683
A genetic linkage map of cassava has been generated with total of 355 molecular markers, 231 amplified fragment length polymorphisms
(AFLPs), 41 simple sequence repeats (SSRs), 48 sequence-related amplified polymorphisms (SRAPs), and 35 expressed sequence
tag (EST)-SSRs segregating from an F1 population of an intraspecific cross between SC6, as female parent, and Mianbao, as male parent. The genetic map consisted
of 18 linkage groups and spanned a 1,707.9 cM genetic distance, with the average marker interval being 4.81 cM. Thirty-five
EST-SSR markers that were developed by our laboratory were mapped onto this map. The genetic map generated in this study will
enrich the information of structural genomics in cassava, facilitating to identify quantitative trait loci (QTL) of interest
and to serve as a useful complement for construction of an integrated map in the future. 相似文献
3.
Fuli Liu Xiuliang Wang Jidong Liu Wandong Fu Delin Duan Yingxia Yang 《Journal of phycology》2009,45(5):1228-1233
To establish a molecular‐marker‐assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F2 cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P ≤ 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular‐marker‐assisted breeding for Laminaria. 相似文献
4.
Fernandez-Silva I Eduardo I Blanca J Esteras C Picó B Nuez F Arús P Garcia-Mas J Monforte AJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,118(1):139-150
We report the development of 158 primer pairs flanking SSR motifs in genomic (gSSR) and EST (EST-SSR) melon sequences, all
yielding polymorphic bands in melon germplasm, except one that was polymorphic only in Cucurbita species. A similar polymorphism level was found among EST-SSRs and gSSRs, between dimeric and trimeric EST-SSRs, and between
EST-SSRs placed in the open reading frame or any of the 5′- or 3′-untranslated regions. Correlation between SSR length and
polymorphism was only found for dinucleotide EST-SSRs located within the untranslated regions, but not for trinucleotide EST-SSRs.
Transferability of EST-SSRs to Cucurbita species was assayed and 12.7% of the primer pairs amplified at least in one species, although only 5.4% were polymorphic.
A set of 14 double haploid lines from the cross between the cultivar “Piel de Sapo” and the accession PI161375 were selected
for the bin mapping approach in melon. One hundred and twenty-one SSR markers were newly mapped. The position of 46 SSR loci
was also verified by genotyping the complete population. A final bin-map was constructed including 80 RFLPs, 212 SSRs, 3 SNPs
and the Nsv locus, distributed in 122 bins with an average bin length of 10.2 cM and a maximum bin length of 33 cM. Map density
was 4.2 cM/marker or 5.9 cM/SSR.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
5.
A high‐density,SNP‐based consensus map of tetraploid wheat as a bridge to integrate durum and bread wheat genomics and breeding 下载免费PDF全文
Marco Maccaferri Andrea Ricci Silvio Salvi Sara Giulia Milner Enrico Noli Pier Luigi Martelli Rita Casadio Eduard Akhunov Simone Scalabrin Vera Vendramin Karim Ammar Antonio Blanco Francesca Desiderio Assaf Distelfeld Jorge Dubcovsky Tzion Fahima Justin Faris Abraham Korol Andrea Massi Anna Maria Mastrangelo Michele Morgante Curtis Pozniak Amidou N'Diaye Steven Xu Roberto Tuberosa 《Plant biotechnology journal》2015,13(5):648-663
6.
High-density genetic map of durum wheat × wild emmer wheat based on SSR and DArT markers 总被引:1,自引:1,他引:0
Peleg Z Saranga Y Suprunova T Ronin Y Röder MS Kilian A Korol AB Fahima T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(1):103-115
A genetic linkage map of tetraploid wheat was constructed based on a cross between durum wheat [Triticum turgidum ssp. durum (Desf.) MacKey] cultivar Langdon and wild emmer wheat [T. turgidum ssp. dicoccoides (K?rn.) Thell.] accession G18-16. One hundred and fifty-two single-seed descent derived F(6) recombinant inbred lines (RILs) were analyzed with a total of 690 loci, including 197 microsatellite and 493 DArT markers. Linkage analysis defined 14 linkage groups. Most markers were mapped to the B-genome (60%), with an average of 57 markers per chromosome and the remaining 40% mapped to the A-genome, with an average of 39 markers per chromosome. To construct a stabilized (skeleton) map, markers interfering with map stability were removed. The skeleton map consisted of 307 markers with a total length of 2,317 cM and average distance of 7.5 cM between adjacent markers. The length of individual chromosomes ranged between 112 cM for chromosome 4B to 217 cM for chromosome 3B. A fraction (30.1%) of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on chromosomes 1A, 1BL, 2BS, 3B, and 4B. DArT markers showed high proportion of clustering, which may be indicative of gene-rich regions. Three hundred and fifty-two new DArT markers were mapped for the first time on the current map. This map provides a useful groundwork for further genetic analyses of important quantitative traits, positional cloning, and marker-assisted selection, as well as for genome comparative genomics and genome organization studies in wheat and other cereals. 相似文献
7.
8.
Sishen Li Jizeng Jia Xianyun Wei Xiaocun Zhang Linzhi Li Haimei Chen Yuding Fan Haiyan Sun Xinhua Zhao Tiandong Lei Yunfong Xu Fangshan Jiang Honggang Wang Lihui Li 《Molecular breeding : new strategies in plant improvement》2007,20(2):167-178
A new genetic linkage map was constructed based on recombinant inbred lines (RILs) derived from the cross between the Chinese
winter wheat (Triticum aestivum L.) varieties, Chuang 35050 and Shannong 483 (ChSh). The map included 381 loci on all the wheat chromosomes, which were composed
of 167 SSR, 94 EST-SSR, 76 ISSR, 26 SRAP, 15 TRAP, and 3 Glu loci. This map covered 3636.7 cM with 1327.7 cM (36.5%), 1485.5 cM (40.9%), and 823.5 cM (22.6%) for A, B, and D genome,
respectively, and contained 13 linkage gaps. Using the RILs and the map, we detected 46 putative QTLs on 12 chromosomes for
grain yield (GY) per m2, thousand-kernel weight (TKW), spike number (SN) per m2, kernel number per spike (KNS), sterile spikelet number per spike (SSS), fertile spikelet number per spike (FSS), and total
spikelet number per spike (TSS) in four environments. Each QTL explained 4.42–70.25% phenotypic variation. Four QTL cluster
regions were detected on chromosomes 1D, 2A, 6B, and 7D. The most important QTL cluster was located on chromosome 7D near
the markers of Xwmc31, Xgdm67, and Xgwm428, in which 8 QTLs for TKW, SN, SSS and FSS were observed with very high contributions (27.53–67.63%). 相似文献
9.
C. Du G. E. Hart 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):645-653
Triticum turgidum L. var ‘durum’ cv ‘Langdon’-T. t. var ‘dicoccoides’ chromosome 6A and 6B recombinant substitution lines (RSLs) and a F2 population derived from a ‘Langdon’-T. t. var ‘dicoccoides’ disomic chromosome 6A substitution lineבLangdon’ cross were analyzed with the objective of markedly increasing
the number of markers assigned to and the resolution of previously constructed 6A and 6B linkage maps. Fifty-seven markers
were added to the 6A RSL-population map, which now consists of 73 markers that span 111 cM, and 40 markers were added to the
6B RSL-population map, which now consists of 56 markers that span 123 cM. With the exception of 2 6B loci, all of the loci
on the two RSL-population maps were ordered at a LOD score ≥3.0. Thirty-seven orthologous markers were mapped in the two chromosomes
and colinearity between them is strongly indicated. The 6A RSL-population map and the F2-population map are highly similar, indicating that the former population, which consists of 66 lines, can be reliably used
for mapping, as was previously demonstrated for the 6B RSL population. In the absence of selection and genetic drift, the
lines in a RSL population, except at loci in the substituted/recombined chromosome, should be near-isogenic. An unexpected
finding was that at least 26 and possibly 29 of the RFLPs detected in the RSL populations (18% of the markers analyzed) are
not located in the substituted/recombined chromosomes. Linkage analysis of the markers disclosed that at least 19 of them
are located in six or seven segments that span approximately 10 cM and 17 cM of the genetic lengths of 6B and 6A, respectively,
in the 6A and 6B RSL populations, respectively, a finding that suggests that 40 or more alien segments spanning 8–15% of the
genetic length of the 13 unsubstituted chromosomes are present in both of the RSL populations. Alien alleles are fixed in
many RSLs for most of the markers, in most cases at a frequency consistent with theoretical expectations. Highly distorted
segregation favoring the alien allele was detected for all of the markers in 2 of the segments, however. Nine of the markers
were among those mapped in the substituted/recombined chromosomes; the linkage data obtained for the other 10 was sufficient
to assign them to approximate map positions.
Received: 12 June 1997 / Accepted: 6 October 1997 相似文献
10.
Frelichowski JE Palmer MB Main D Tomkins JP Cantrell RG Stelly DM Yu J Kohel RJ Ulloa M 《Molecular genetics and genomics : MGG》2006,275(5):479-491
Fine mapping and positional cloning will eventually improve with the anchoring of additional markers derived from genomic
clones such as BACs. From 2,603 new BAC-end genomic sequences from Gossypium hirsutum Acala ‘Maxxa’, 1,316 PCR primer pairs (designated as MUSB) were designed to flank microsatellite or simple sequence repeat
motif sequences. Most (1164 or 88%) MUSB primer pairs successfully amplified DNA from three species of cotton with an average
of three amplicons per marker and 365 markers (21%) were polymorphic between G.
hirsutum and G. barbadense. An interspecific RIL population developed from the above two entries was used to map 433 marker loci and 46 linkage groups
with a genetic distance of 2,126.3 cM covering approximately 45% of the cotton genome and an average distance between two
loci of 4.9 cM. Based on genome-specific chromosomes identified in G. hirsutum tetraploid (A and D), 56.9% of the coverage was located on the A subgenome while 39.7% was assigned to the D subgenome in
the genetic map, suggesting that the A subgenome may be more polymorphic and recombinationally active than originally thought.
The linkage groups were assigned to 23 of the 26 chromosomes. This is the first genetic map in which the linkage groups A01
and A02/D03 have been assigned to specific chromosomes. In addition the MUSB-derived markers from BAC-end sequences markers
allows fine genetic and QTL mapping of important traits and for the first time provides reconciliation of the genetic and
physical maps. Limited QTL analyses suggested that loci on chromosomes 2, 3, 12, 15 and 18 may affect variation in fiber quality
traits. The original BAC clones containing the newly mapped MUSB that tag the QTLs provide critical DNA regions for the discovery
of gene sequences involved in biological processes such as fiber development and pest resistance in cotton.
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. 相似文献
11.
Blanco A Simeone R Cenci A Gadaleta A Tanzarella OA Porceddu E Salvi S Tuberosa R Figliuolo G Spagnoletti P Röder MS Korzun V 《Cellular & molecular biology letters》2004,9(3):529-541
A set of recombinant inbred lines (RIL) derived from a cross between the cultivar Messapia of durum wheat (Triticum turgidum var. durum) and the accession MG4343 of T. turgidum var. dicoccoides was analysed to increase the number of assigned markers and the resolution of the previously constructed genetic linkage map. An updated map of the durum wheat genome consisting of 458 loci was constructed. These loci include 261 Restriction Fragment Length Polymorphisms (RFLPs), 91 microsatellites (Simple Sequence Repeats, SSRs), 87 Amplified Fragment Length Polymorphisms (AFLPs), two ribosomal genes, and nine biochemical (seven seed storage proteins and two isozymes) and eight morphological markers. The loci were mapped on all 14 chromosomes of the A and B genomes, and covered a total distance of 3038.4 cM with an average distance of 6.7 cM between adjacent markers. The molecular markers were evenly distributed between the A and the B genomes (240 and 218 markers, respectively). An additional forty loci (8.8%) could not be assigned to a specific linkage group. A fraction (16.4%) of the markers significantly deviated from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on the 1B, 2A, 2B, 3A, 4A, 7A and 7B chromosomes. The genetic lengths of the chromosomes range from 148.8 cM (chromosome 6B) to 318.0 cM (chromosome 2B) and approximately concur with their physical lengths. Chromosome 2B has the largest number of markers (47), while the chromosomes with the fewest markers are 3A and 6B (23). There are two gaps larger than 40 cM on chromosomes 2A and 3B. The durum wheat map was compared with the published maps of bread and durum wheats; the order of most common RFLP and SSR markers on the 14 chromosomes of the A and B genomes were nearly identical. A core-map can be extracted from the high-density Messapia x dicoccoides map and a subset of uniformly distributed markers can be used to detect and map quantitative trait loci. 相似文献
12.
Ulloa M Meredith WR Shappley ZW Kahler AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):200-208
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 相似文献
13.
Cassava (Manihot esculenta) is an economically important crop that is grown in tropical and sub-tropical regions. Use of molecular technology for genetic
improvement of cassava has been limited by the lack of a large set of DNA markers and a genetic map. Therefore, the aims here
were to develop additional simple sequence repeat (SSR) markers from the public expressed sequence tags (ESTs), and to construct
a genetic linkage map. In this study, we designed 425 EST-SSR markers from sequences obtained from the cassava EST database
in GenBank, and integrated them with 667 SSR markers from a microsatellite-enriched genomic sequence received from the International
Center for Tropical Agriculture (CIAT). Of these, 107 EST-SSR and 500 genomic SSR primer pairs showed polymorphic patterns
when screened in two cassava varieties, Hauy Bong 60 and Hanatee, which were used as female and male parental lines, respectively.
Within the 107 and 500 primer pairs, 81 and 226 EST-SSR and SSR primer pairs were successfully genotyped with 100 samples
of F1 progeny, respectively. The results showed 20 linkage groups consisting of 211 markers—56 EST-SSR and 155 SSR markers—spanning
1,178 cM, with an average distance between markers of 5.6 cM and about 11 markers per linkage group. These novel EST-SSR markers
provided genic PCR-based co-dominant markers that were useful, reliable and economical. The EST-SSRs were used together with
SSR markers to construct the cassava genetic linkage map which will be useful for the identification of quantitative trait
loci controlling the traits of interest in cassava breeding programs. 相似文献
14.
Pacific white shrimp (Litopenaeus vannamei) is the leading species farmed in the Western Hemisphere and an economically important aquaculture species in China. In this
project, a genetic linkage map was constructed using amplified fragment length polymorphism (AFLP) and microsatellite markers.
One hundred and eight select AFLP primer combinations and 30 polymorphic microsatellite markers produced 2071 markers that
were polymorphic in either of the parents and segregated in the progeny. Of these segregating markers, 319 were mapped to
45 linkage groups of the female framework map, covering a total of 4134.4 cM; and 267 markers were assigned to 45 linkage
groups of the male map, covering a total of 3220.9 cM. High recombination rates were found in both parental maps. A sex-linked
microsatellite marker was mapped on the female map with 6.6 cM to sex and a LOD of 17.8, two other microsatellite markers
were also linked with both 8.6 cM to sex and LOD score of 14.3 and 16.4. The genetic maps presented here will serve as a basis
for the construction of a high-resolution genetic map, quantitative trait loci (QTLs) detection, marker-assisted selection
(MAS) and comparative genome mapping. 相似文献
15.
SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum
Kim C Zhang D Auckland SA Rainville LK Jakob K Kronmiller B Sacks EJ Deuter M Paterson AH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(7):1325-1338
We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental
species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived
SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication.
While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to
improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum. 相似文献
16.
A genetic linkage map of durum wheat 总被引:20,自引:6,他引:14
A. Blanco M. P. Bellomo A. Cenci C. De Giovanni R. D’Ovidio E. Iacono B. Laddomada M. A. Pagnotta E. Porceddu A. Sciancalepore R. Simeone O. A. Tanzarella 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):721-728
A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from
a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR
(polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the
Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total
of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and
the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%)
of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were
found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using
several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving
chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful
tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for
studies of genome organisation in small grain cereal species.
Received: 5 January 1998 / Accepted: 31 March 1998 相似文献
17.
Harsh Raman Jessica Dalton‐Morgan Simon Diffey Rosy Raman Salman Alamery David Edwards Jacqueline Batley 《Plant biotechnology journal》2014,12(7):851-860
An Illumina Infinium array comprising 5306 single nucleotide polymorphism (SNP) markers was used to genotype 175 individuals of a doubled haploid population derived from a cross between Skipton and Ag‐Spectrum, two Australian cultivars of rapeseed (Brassica napus L.). A genetic linkage map based on 613 SNP and 228 non‐SNP (DArT, SSR, SRAP and candidate gene markers) covering 2514.8 cM was constructed and further utilized to identify loci associated with flowering time and resistance to blackleg, a disease caused by the fungus Leptosphaeria maculans. Comparison between genetic map positions of SNP markers and the sequenced Brassica rapa (A) and Brassica oleracea (C) genome scaffolds showed several genomic rearrangements in the B. napus genome. A major locus controlling resistance to L. maculans was identified at both seedling and adult plant stages on chromosome A07. QTL analyses revealed that up to 40.2% of genetic variation for flowering time was accounted for by loci having quantitative effects. Comparative mapping showed Arabidopsis and Brassica flowering genes such as Phytochrome A/D, Flowering Locus C and agamous‐Like MADS box gene AGL1 map within marker intervals associated with flowering time in a DH population from Skipton/Ag‐Spectrum. Genomic regions associated with flowering time and resistance to L. maculans had several SNP markers mapped within 10 cM. Our results suggest that SNP markers will be suitable for various applications such as trait introgression, comparative mapping and high‐resolution mapping of loci in B. napus. 相似文献
18.
James W. Borrone J. Steven Brown Cecile L. Tondo Margarita Mauro-Herrera David N. Kuhn Helen A. Violi Robert T. Sautter Raymond J. Schnell 《Tree Genetics & Genomes》2009,5(4):553-560
Recent enhancement of the pool of known molecular markers for avocado has allowed the construction of the first moderately
dense genetic map for this species. Over 300 SSR markers have been characterized and 163 of these were used to construct a
map from the reciprocal cross of two Florida cultivars 'Simmonds' and 'Tonnage'. One hundred thirty-five primer pairs amplified
163 usable loci with 20 primer pairs amplifying more than one locus. 'Tonnage' was heterozygous for 152 (93%) loci, whereas
'Simmonds' was heterozygous for 64 (39%). Null alleles were identified at several loci. Linkage maps were produced for both
reciprocal crosses and combined to generate a composite linkage map for the F1 population of 715 individuals. The composite map contains 12 linkage groups. Linkage groups ranged in size from 157.3 cM
(LG2) to 2.4 cM (LG12) and the number of loci mapped per group ranged from 29 (LG1) to two (LG12). The total map length was
1,087.4 cM. Only seven markers were observed to have segregation distortion (α ≤ 0.05) across both sub-composite (reciprocal) maps. Phenotypic data from traits of horticultural interest are currently
being collected on this population with the ultimate goal of identifying useful quantitative trait loci and the development
of a marker-assisted selection program. 相似文献
19.
Kroemer JA Coates BS Nusawardani T Rider SD Fraser LM Hellmich RL 《Molecular genetics and genomics : MGG》2011,286(1):37-56
Males are homogametic (ZZ) and females are heterogametic (WZ) with respect to the sex chromosomes in many species of butterflies
and moths (insect order Lepidoptera). Genes on the Z chromosome influence traits involved in larval development, environmental
adaptation, and reproductive isolation. To facilitate the investigation of these traits across Lepidoptera, we developed 43
degenerate primer pairs to PCR amplify orthologs of 43 Bombyx mori Z chromosome-linked genes. Of the 34 orthologs that amplified by PCR in Ostrinia nubilalis, 6 co-segregated with the Z chromosome anchor markers kettin (ket) and lactate dehydrogenase (ldh), and produced a consensus genetic linkage map of ~89 cM in combination with 5 AFLP markers. The O. nubilalis and B. mori Z chromosomes are comparatively co-linear, although potential gene inversions alter terminal gene orders and a translocation
event disrupted synteny at one chromosome end. Compared to B. mori orthologs, O. nubilalis Z chromosome-linked genes showed conservation of tissue-specific and growth-stage-specific expression, although some genes
exhibited species-specific expression across developmental stages or tissues. The O. nubilalis Z chromosome linkage map provides new tools for isolating quantitative trait loci (QTL) involved in sex-linked traits that
drive speciation and it exposes genome rearrangements as a possible mechanism for differential gene regulation in Lepidoptera. 相似文献
20.
Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.) 总被引:1,自引:0,他引:1
Zheng-Sheng Zhang Mei-Chun Hu Jian Zhang Da-Jun Liu Jing Zheng Ke Zhang Wei Wang Qun Wan 《Molecular breeding : new strategies in plant improvement》2009,24(1):49-61
To facilitate marker assisted selection, there is an urgent need to construct a saturated genetic map of upland cotton (Gossypium hirsutum L.). Four types of markers including SSR, SRAP, morphological marker, and intron targeted intron–exon splice junction (IT-ISJ)
marker were used to construct a linkage map with 270 F2:7 recombinant inbred lines derived from an upland cotton cross (T586 × Yumian 1). A total of 7,508 SSR, 740 IT-ISJ and 384
SRAP primer pairs/combinations were used to screen for polymorphism between the two mapping parents, and the average polymorphisms
of three types of molecular markers represented 6.8, 6.6 and 7.0%, respectively. The polymorphic primer pairs/combinations
and morphological markers were used to genotype 270 recombinant inbred lines, and a map including 604 loci (509 SSR, 58 IT-ISJ,
29 SRAP and 8 morphological loci) and 60 linkage groups was constructed. The map spanned 3,140.9 cM with an average interval
of 5.2 cM between two markers, approximately accounting for 70.6% of the cotton genome. Fifty-four of 60 linkage groups were
ordered into 26 chromosomes. Multiple QTL mapping was used to identify QTL for fiber quality traits in five environments,
and thirteen QTL were detected. These QTL included four for fiber length (FL), two for fiber strength (FS), two for fiber fineness (FF), three for fiber length uniformity (FU), and two for fiber elongation (FE), respectively. Each QTL explained between 7.4 and 43.1% of phenotypic variance. Five out of thirteen QTL (FL1 and FU1 on chromosome 6, FL2, FU2 and FF1 on chromosome7) were detected in five environments, and they explained more than 20% of the phenotypic variance. Eleven QTL
were distributed on A genome, while the other two on D genome. 相似文献