| 大豆胰蛋白酶抑制剂对棉铃虫幼虫消化生理和生长发育的影响 |
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| 引用本文： | 王琛柱,项秀芬.大豆胰蛋白酶抑制剂对棉铃虫幼虫消化生理和生长发育的影响[J].昆虫学报,1995,38(3):272-277. |
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| 作者姓名： | 王琛柱 项秀芬 |
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| 作者单位： | 中国科学院动物研究所北京 |
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| 摘 要： | 本研究根据棉铃虫Helicotverpa ormigera(Hubner)幼虫中肠蛋白酶在离体条件下对蛋白酶抑制剂的反应，选择具有较强抑制作用的大豆胰蛋白酶抑制剂，以0.21-4.2%(干重)的浓度配入幼虫人工饲料，测定了幼虫短期和长期取食这些饲料引起的中肠类胰蛋白酶、类胰凝乳蛋白酶和总蛋白酶活力的变化和生长抑制效应。短期取食抑制剂的幼虫，中肠弱碱性类胰蛋白酶活力显著增高，在4.2%。浓度下比对照高出21%；强碱性类胰蛋白酶、类胰凝乳蛋白酶和总蛋白酶活力显著降低，生长发育受到明显抑制。长期取食低浓度(0.84%)抑制剂的幼虫，弱碱性类胰蛋白酶和类胰凝乳蛋白酶活力显著增高，强碱性类胰蛋白酶活力显著降低。总蛋白酶活力变化不显著；长期取食高浓度(4.2%)抑制剂的幼虫，强碱性类胰蛋白酶和总蛋白酶活力显著降低，其它酶活力变化不显著。抑制剂随浓度的增高对幼虫生长的抑制作用加强，但浓度高于0.84%后，抑制强度的变化减小。据此作者认为，蛋白酶抑制剂对昆虫抗营养效应在于它对蛋白酶的激活和抑制作用，从而导致各种蛋白酶间的协调性破坏，昆虫消化过程受阻，影响生长发育。
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| 关 键 词： | 大豆胰蛋白酶抑制剂 棉铃虫 蛋白酶 |
EFFECT OF SOYBEAN TRYPSIN INHIBITOR ON THE GROWTH AND DIGESTIVE PHYSIOLOGY OF HELICOVERPA ARMIGERA LARVAE |
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| Wang Chenzhu,Xiang Xiufen,Zhang Shufang,Qin Junde.EFFECT OF SOYBEAN TRYPSIN INHIBITOR ON THE GROWTH AND DIGESTIVE PHYSIOLOGY OF HELICOVERPA ARMIGERA LARVAE[J].Acta Entomologica Sinica,1995,38(3):272-277. |
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| Authors: | Wang Chenzhu Xiang Xiufen Zhang Shufang Qin Junde |
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| Abstract: | Based on the in vitro effect on protease activity of larval midgut,soybean trypsin inhibitor (STI) was suggested as the potential resistant agent to cotton bollworm, Helicoverpa armigera.It was incorPOrated in the larval artificial diet at 0.84-4.2% (dry weight) to determine its acute and chronic effects on the growth and in vivo midgut protease activity of cotton bollworm. Ingestion of 4.2%STI in short term resulted in reduced larval growth rate, increased low alkaline trypsin-like enzyme activity, decreased high alkaline trypsin-like enzyme activity.decreased chymotrypsin-like enzyme activity, and decreased total proteolysis. When the larvae chronically ingested 0.84% STI, low alkaline trypsin-like and chymotrypsin-like enzyme activities were significantly elevated, high alkaline trypsin-like enzyme activity was markedly declined, and total proteolysis had no significant change.When the larvae chronically ingested 4.2% STI, high alkaline trypsin-like enzyme activity and total proteolysis were significantly decreased. but there was no significant elevation of low alkaline trypsin-like and chymotrypsin-like enzyme activities. Retarding effect of STI on growth of the larvae was significant but not proportional to the dosage of STI. Thus, the mechanism of anti-nutritional action of proteinase inhibitor seems to be complicated, and pernicious hyperproduction of some protease may be only one aspect of the action. Influence of cooperative proteases activity may still be the basis in mode of action. |
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| Keywords: | Soybean trypsin inhibitor Helicoverpa armigera protease |
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