Synthesis of wax esters by a cell-free system from barley (Hordeum vulgare L.) |
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Authors: | Pinarosa Avato |
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Institution: | (1) Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark;(2) Present address: Dipartimento di Chimica Organica, Università di Pavia, Viale Taramelli 10, I-27100 Pavia, Italy |
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Abstract: | Experimental evidence for a membranebound microsomal ester synthetase from Bonus barley primary leaves is reported. The results are consistent with at least two mechanisms for the synthesis of barley wax esters: an acyl-CoA-fattyalcohol-transacylase-type reaction and an apparent direct esterification of alcohols with fatty acids. Biosynthesis of wax esters was not specific with regard to the chain length of the tested alcohols. The microsomal preparation readily catalyzed the esterification of C16-, C18-, C22- or C24-labelled alcohols with fatty acids of endogenous origin. Exogenous long-chain alcohols were exclusively incorporated into the alkyl moieties of the esters. Addition of ATP, CoA and-or free fatty acids was not effective in stimulating or depressing the esterifying activity of the microsomal fraction. Partial solubilization of the ester synthetase was obtained using phosphate-buffered saline.Abbreviations P
pellet
- PBS
phosphate-buffered saline
- S
supernatant
- SDS
sodium dodecyl sulphate |
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Keywords: | Alcohol (long chain) Ester synthetase Hordeum (wax esters) Wax ester biosynthesis |
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