Enhanced mannanase production by submerged culture of Aspergillus niger NCH-189 using defatted copra based media |
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| Institution: | 1. Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;2. Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University (UNESP), Cristóvão Colombo, 2265 São José do Rio Preto, Brazil;3. Fungal Genetics and Biotechnology, Department of Microbiology, University of Helsinki, Viikinkaari 9, 00790 Helsinki, Finland;1. Microarray Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Luang, Pathum Thani, Thailand;2. Food Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Luang, Pathum Thani, Thailand |
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| Abstract: | Copra and other mannans including locust bean gum, guar gum, konjac flour, copra and defatted copra were used to produce extracellular mannanase by shaken flask cultures of Aspergillus niger NCH-189 in this study. The best carbon source for mannanase production was defatted copra, which provided more nitrogen source and mannan content. The peak mannanase activity at 28 U ml−1 was obtained on the day 3 at 30 °C, which was four times of those obtained from other carbon sources. Presence of oil in copra depressed the mannanase production of the fungus and the amount should be less than 3% (w/w). The copra suspension could be sequentially treated by boiling and refrigeration, followed by using n-hexane to remove copra oil. |
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