Effect of cadmium on lipid composition of Aureobasidium pullulans grown with added extracellular polysaccharides |
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| Institution: | 1. Laboratoire de Chimie Physique UMR 8000, Univ. Paris-Sud, CNRS, Université Paris Saclay, 91405 Orsay Cedex, France;2. Synchrotron SOLEIL, Campus Paris-Saclay, 91192 Gif-sur-Yvette Cedex, France;1. Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, P.R. China;2. University of Chinese Academy of Sciences, Beijing 100049, P.R. China;3. Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of Environment and Ecology, Xiamen University, Xiamen 361102, P.R. China;4. Division of Life Science, The Hong Kong University of Science and Technology (HKUST), Clearwater Bay, Kowloon, Hong Kong;1. College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China;2. Department of Biology, Shantou University, Shantou 515063, China;3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 266003 Qingdao, China |
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| Abstract: | Effect of cadmium on the growth and lipid composition in three Aureobasidium pullulans strains grown in the presence and absence of extracellular polysaccharides was studied. Strains tolerated up to 1 mM Cd2+ in medium, but addition of either pectin or pullulan to medium increased tolerance to up to 2 mM Cd2+. While cadmium enhanced lipid accumulation notably only in strains of CCY 27-1-98 and CCY 27-1-111, strain CCY 27-1-115 displayed considerably high levels of sterols. Cadmium increased fatty acid unsaturation rapidly in phosphatidylethanolamine in all strains, owing to elevated levels of linoleic acid in this lipid fraction. On the other hand, Cd2+ ions significantly lowered conversion of oleic acid to linoleic acid in phosphatidylcholine only in the strain CCY 27-1-115. Nevertheless, addition of either pullulan or pectin amplified the ratio of C18:C16 fatty acids and the degree of fatty acid unsaturation in all membrane lipids as a result of induced biotransformation of stearate to oleate and linoleate. It was concluded that more saturated membranes with preferred C18 fatty acyl chains and high sterol amounts in the strain CCY 27-1-115 might be connected with its lower capacity to form pullulan as a protective metabolite against heavy metals compared with the other two strains. |
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