MicroRNA‐10b regulates the renewal of spermatogonial stem cells through Kruppel‐like factor 4 |
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| Authors: | Jiang Li Xiang Liu Xiaopeng Hu Geng G Tian Wenzhi Ma Xiuying Pei Yanrong Wang Ji Wu |
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| Institution: | 1. Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, China;2. Bio‐X Institutes, Shanghai Jiao Tong University, Shanghai, China;3. Shanghai Key Laboratory of Reproductive Medicine, Shanghai, China |
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| Abstract: | MicroRNAs (miRs) are functionally important in spermatogenesis, which is the self‐renewal or differentiation of spermatogonial stem cells (SSCs). Here, we report a novel role for miR‐10b in regulating the self‐renewal of mouse SSCs. We showed that miR‐10b was highly expressed in mouse SSCs in vitro and enhanced SSC proliferation. Knockdown of miR‐10b significantly increased the apoptosis of SSCs compared with controls. Kruppel‐like factor 4 was found to be a target gene of miR‐10b in the enhancement of SSC proliferation. These findings further our understanding of the self‐renewal and differentiation of SSCs and provide a basis for the diagnosis, treatment, and prevention of male infertility. |
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| Keywords: | KLF4 miR10b self‐renewal spermatogonial stem cells |
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