Exploration of Phytoconstituents from Mussaenda roxburghii and Studies of Their Antibiofilm Effect |
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| Authors: | Arpita Sarkar Antu Das Padmani Sandhu Biswanath Dinda Yusuf Akhter Surajit Bhattacharjee Utpal Ch. De |
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| Affiliation: | 1. Department of Molecular Biology & Bioinformatics, Tripura University, Suryamaninagar, Tripura, India;2. Centre for Computational Biology and Bioinformatics, School of Life Sciences, Central University of Himachal Pradesh, Shahpur, Kangra District, Himachal Pradesh, India;3. Department of Chemistry, Tripura University, Suryamaninagar, Tripura, India |
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| Abstract: | In the context of ethno botanical importance with no phytochemical investigations, Mussaenda roxburghii have been investigated to explore it's phytoconstituents and studies of their antibiofilm activity. Four compounds have been isolated from the aerial parts of this plant and were characterized as 2α,3β,19α,23‐tetrahydroxyurs‐12‐en‐28‐oic acid ( 1 ), β‐sitosterol glucoside ( 4 ), lupeol palmitate ( 5 ), and myoinositol ( 6 ). All these compounds were tested for antibacterial and antibiofilm activity against Pseudomonas aeruginosa. Compound 1 exhibited three times more antibiofilm activity with minimum inhibitory concentration (MIC) at 0.74 mm compared to that of streptomycin. Molecular docking studies exhibited a very high binding affinity of 1 with P. aeruginosa quorum sensing proteins and motility associated proteins viz. LasR and PilB, PilY1, PilT, respectively. Compound 1 was also found to be non‐cytotoxic against sheep RBC and murine peritoneal macrophages at selected sub‐MIC doses. |
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| Keywords: |
Mussaenda roxburghii
23‐Hydroxytormentic acid Antibacterial activity Antibiofilm Triterpene acid Pentacyclic triterpene |
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