首页 | 本学科首页   官方微博 | 高级检索  
     


NDRG1 is important to maintain the integrity of airway epithelial barrier through claudin‐9 expression
Authors:Yasuhiro Gon  Shuichiro Maruoka  Hiroyuki Kishi  Yutaka Kozu  Kuroda Kazumichi  Yasuyuki Nomura  Ikuko Takeshita  Takeshi Oshima  Shu Hashimoto
Affiliation:1. Division of Respiratory Medicine, Nihon University School of Medicine, Itabashiku, Tokyo 173‐8610, Japan;2. Division of Otolaryngology, Nihon University School of Medicine, Itabashiku, Tokyo 173‐8610, Japan;3. Division of Microbiology, Nihon University School of Medicine, Itabashiku, Tokyo 173‐8610, Japan
Abstract:Impairment of epithelial barrier integrity caused by environmental triggers is associated with the pathogenesis of airway inflammation. Using human airway epithelial cells, we attempted to identify molecule(s) that promote airway epithelial barrier integrity. Microarray analyses were conducted using the Affimetrix human whole genome gene chip, and we identified the N‐myc downstream‐regulated gene 1 (NDRG1) gene, which was induced during the development of the epithelial cell barrier. Immunohistochemical analysis revealed strong NDRG1 expression in ciliated epithelial cells in nasal tissues sampled from patients with chronic rhinosinusitis (CRS), and the low expression of NDRG1 was observed in goblet cells or damaged epithelial cells. NDRG1 gene knockdown with its specific siRNA decreased the transepithelial electrical resistance and increased the dextran permeability. Immunocytochemistry revealed that NDRG1 knockdown disrupted tight junctions of airway epithelial cells. Next, we analyzed the effects of NDRG1 knockdown on the expression of tight and adhesion junction molecules. NDRG1 knockdown significantly decreased only claudin‐9 expression, but did not decrease other claudin family molecules, such as E‐cadherin, and ZO‐1, ‐2, or ‐3. Knockdown of claudin‐9 markedly impaired the barrier function in airway epithelial cells. These results suggest that NDRG1 is important for the barrier integrity in airway epithelial cells.
Keywords:barrier function  chronic rhinosinusitis  claudin‐9  NDRG1  tight junction
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号