罗汉果组织培养中愈伤组织和腋生枝的形成

用罗汉果茎段为外植体,培养在MS+BA1.0+IBA0.1毫克/升培养基上,诱导愈伤组织和芽形成。观察了罗汉果茎段愈伤组织的生长以及腋生枝的形成。罗汉果茎段培养5天后,潜伏腋芽开始萌动和生长。培养10天后罗汉果茎段的基部一端开始膨大。培养20天后产生大量白色疏松的愈伤组织,这时腋生枝已经长成3—6厘米长。培养30天后基部的愈伤组织中有少量瘤状小突起,但再分化形成芽的频率极低。结果表明,罗汉果茎段组培形成的苗均是从腋芽产生的。

THE CALLUS AND AXILLARY SHOOT FORMATION OF LUOHANGUO IN VITRO

The stem explants of Luohanguo (Siraitia grosvenorii) were cultured on MS basal medium supplemented with 1.0mg/l BA and 0.1mg/l IBA for induction of callus and axillary shoot formation. The callus appearance and axillary shoot formation were observated. After 5 days of culture, the concealed bud in the leaf axil began to sprout and grow. After 10 days of culture, the base of the stem began to expand. After 20 days of culture, great many white loose callus appeared in the base of the stem, at the same time axillary shoot grew up some centimetres. After 30 daysof culture, at the base of callus there were light green verrucous granules, but frequency of bud formation by their redifferentiation was lower. It was sugested thatthe shoot formation of Luohanguo in vitro proceeded from axillary buds.