hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells

hSHIP a human SH2-containing inositol-5-phosphatase,acts as a negative regulator of proliferation and survival in hematopoietic cells.Therefore.hSHIP may play a crucial role in suppression of cervical cancer HeLa cells.In this study,pcDNA3.1-hSHIP-GFP plasmid was constructed and transfected into HeLa cells with Lipofectamine2000, stably transfected HeLa cells were established and their responses were investigated by Flow cytometry,MTT, tumorigenicity in nude mice,RT-PCR and ELISA assays.The results showed that the expression of hSHIP significantly induced S-phase arrest.cell growth inhibition.and down-regulation of Aktl/2 mRNA and p-Akt in HeLa cells.Our study supports an important role for hSHIP in suppression of cervical caucer HeLa cells.which may prove to be a novel therapeutic option for non-hematopoietic cancels.