| Novel pleiotropic loci controlling panicle architecture across environments in japonica rice （Oryza sativa L.） |
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| 作者姓名： | Yuan Guo Delin Hong |
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| 作者单位： | [1]State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China [2]Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China |
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| 摘 要： | To identify quantitative trait loci (QTLs) controlling panicle architecture in japonica rice, a genetic map was constructed based on simple sequence repeat (SSR) markers and 254 recombinant inbred lines (RILs) derived from a cross between cultivars Xiushui 79 and C Bao. Seven panicle traits were investigated under three environments. Single marker analysis indicated that a total of 27 SSR markers were highly associated with panicle traits in all the three environments. Percentage of phenotypic variation explained by single locus varied from 2% to 35%. Based on the mixed linear model, a total of 40 additive QTLs for seven panicle traits were detected by composite interval mapping, explaining 1.2%-35% of phenotypic variation. Among the 9 QTLs with more than 10% of explained phenotypic variation, two QTLs were for the number of primary branches per panicle (NPB), two for panicle length (PL), two for spikelet density (SD), one for the number of secondary branches per panicle (NSB), one for secondary branch distribution density (SBD), and one for the number of spikelets per panicle (NS), respectively. qPLSD-9-1 and qPLSD-9-2 were novel pleiotropic loci, showing effects on PL and SD simultaneously. qPLSD-9-1 explained 34.7% of the phenotypic variation for PL and 25.4% of the phenotypic variation for SD, respec- tively. qPLSD-9-2 explained 34.9% and 24.4% of the phenotypic variation for PL and SD, respectively. The C Bao alleles at the both QTLs showed positive effects on PL, and the Xiushui 79 alleles at the both QTLs showed positive effects on SD. Genetic variation of panicle traits are mainly attributed to additive effects. QTL × environment interactions were not significant for additive QTLs and additive × additive QTL pairs.
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| 关 键 词： | 控制体系结构 基因位点 环境 水稻 稻穗 QTLs 表型变异 单标记分析 |
| 收稿时间： | 21 November 2009 |
Novel pleiotropic loci controlling panicle architecture across environments in japonica rice(Oryza sativa L.) |
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| Yuan Guo,Delin Hong.Novel pleiotropic loci controlling panicle architecture across environments in japonica rice(Oryza sativa L.)[J].Journal of Genetics and Genomics,2010,37(8):533-544. |
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| Authors: | Yuan Guo Delin Hong |
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| Institution: | 1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;1. Graduate School of Agriculture, Hokkaido University, Sapporo, Japan;2. International Rice Research Institute, DAPO 7777, Metro Manila, Philippines;3. Philippine Rice Research Institute, Muñoz, Nueva Ecija, Philippines;4. Pangasinan State University Sta. Maria, Pangasinan, Philippines;5. Institute for Sustainable Agro-Ecosystem Services, University of Tokyo, Nishitokyo, Japan;1. Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan;2. Department of Bioscience, Fukui Prefectural University, Yoshida-gun, Fukui 910-1195, Japan;1. State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, Zhejiang, China;2. State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | To identify quantitative trait loci(QTLs)controlling panicle architecture in japonica rice,a genetic map was constructed based on simple sequence repeat(SSR)markers and 254 recombinant inbred lines(RILs)derived from a cross between cultivars Xiushui 79 and C Bao.Seven panicle traits were investigated under three environments.Single marker analysis indicated that a total of 27 SSR markers were highly associated with panicle traits in all the three environments.Percentage of phenotypic variation explained by single locus varied from 2% to 35%.Based on the mixed linear model,a total of 40 additive QTLs for seven panicle traits were detected by composite interval mapping,explaining 1.2%-35% of phenotypic variation.Among the 9 QTLs with more than 10% of explained phenotypic variation,two QTLs were for the number of primary branches per panicle(NPB),two for panicle length(PL),two for spikelet density(SD),one for the number of secondary branches per panicle(NSB),one for secondary branch distribution density(SBD),and one for the number of spikelets per panicle(NS),respectively,qPLSD-9-1 and qPLSD-9-2 were novel pleiotropic loci,showing effects on PL and SD simultaneously,qPLSD-9-1 explained 34.7% of the phenotypic variation for PL and 25.4% of the phenotypic variation for SD,respectively,qPLSD-9-2 explained 34.9% and 24.4% of the phenotypic variation for PL and SD,respectively.The C Bao alleles at the both QTLs showed positive effects on PL,and the Xiushui 79 alleles at the both QTLs showed positive effects on SD.Genetic variation of panicle traits are mainly attributed to additive effects.QTL × environment interactions were not significant for additive QTLs and additive × additive QTL pairs. |
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| Keywords: | japonica rice simple sequence repeat(SSR) panicle traits quantitative trait locus(QTL) pleiotropic locus |
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