Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes |
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| Authors: | Fang Kong Cailin Ge Xiaoping Fang Rod J.Snowdon Youping Wang |
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| Affiliation: | [1]College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China [2]Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China [3]Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany |
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| Abstract: | The diploid species Brassica rapa(genome AA)and B.oleracea(genome CC)were compared by fuU-seale proteome analyses of seedling.A total of 28.2% of the proteins was common to both species,indicating the existence of a basal or ubiquitous proteome.How-ever,a number of discriminating proteins(32.0%)and specific proteins(39.8%)of the Brassica A and C genomes,respectively,were identified,which could represent potentially species-specific functions.Based on these A or C genome-specific proteins,a number of PCR-based markers to distinguish B.rapa and B.oleracea species were also developed. |
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| Keywords: | Brassica rapa Brassica oleracea proteomics molecular marker |
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