The effect of repeated tryptophan administration on body weight, food intake, brain lipid peroxidation and serotonin immunoreactivity in mice |
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Authors: | Şule Coşkun Ph.D. Çiğdem Özer Bilge Gönül Gülnur Take Deniz Erdoğan |
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Affiliation: | (1) Department of Biology, Art and Science Faculty, Gazi University, Ankara, Turkey;(2) Department of Physiology, Faculty of Medicine, Gazi University, Ankara, Turkey;(3) Department of Histology, Faculty of Medicine, Gazi University, Ankara, Turkey;(4) Science and Art Faculty, Department of Biology, Physiology, Teknikokullar, Gazi University, Ankara, Turkey |
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Abstract: | Tryptophan as a circulating precursor of serotonin (5-HT) may suppress food intake and body weight. Tryptophan administration can enhance the generation of reactive oxygen species (ROS) by inducing oxidative pathway in vivo and in vitro. We have examined the effect of repeated tryptophan administration on food consumption, body weight, brain lipid peroxidation and 5-HT immunoreactivity. Tryptophan was given at the dose of 100 mg/kg/24 hr in 0.2 ml saline solution i.p. for 7 days to mice. Control mice received 0.9% NaCL solution at the same manner and volume. Body weights were recorded at the beginning and end of the experiments. Thiobarbituric acid reactive substance (TBARS), the last product of lipid peroxidation, was measured spectrophotometrically. Brain 5-HT levels were determined by the immunohistochemical method. Our findings indicate that the tryptophan suppresses food intake significantly in mice. Body weight decreased and brain TBARS levels increased significantly by repeated tryptophan treatment. Immunohistochemical detection showed that 5-HT levels increased by tryptophan administration. There is a link between increased 5-HT level and oxidative stress by tryptophan administration on brain tissue. Tryptophan at repeated doses should be exercised carefully in clinical practice. |
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Keywords: | brain lipid peroxidation food intake glutathione serotonin immunoreactivity tryptophan |
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