Enhanced production of bioethanol from waste of beer fermentation broth at high temperature through consecutive batch strategy by simultaneous saccharification and fermentation |
| |
Authors: | Waleed Ahmad Khattak Taous Khan Jung Hwan Ha Mazhar Ul-Islam Min-Kyung Kang Joong Kon Park |
| |
Institution: | 1. Department of Chemical Engineering, Kyungpook National University, Daegu 702-701, South Korea;2. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan |
| |
Abstract: | Malt hydrolyzing enzymes and yeast glycolytic and fermentation enzymes in the waste from beer fermentation broth (WBFB) were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). A new ‘one-pot consecutive batch strategy’ was developed for efficient bio-ethanol production by simultaneous saccharification and fermentation (SSF) using WBFB without additional enzymes, microbial cells, or carbohydrates. Bio-ethanol production was conducted in batches using WBFB supernatant in the first phase at 25–67 °C and 50 rpm, followed by the addition of 3% WBFB solid residue to the existing culture broth in the second phase at 67 °C. The ethanol production increased from 50 to 102.5 g/L when bare supernatant was used in the first phase, and then to 219 g ethanol/L in the second phase. The amount of ethanol obtained using this strategy was almost equal to that obtained using the original WBFB containing 25% solid residue at 33 °C, and more than double that obtained when bare supernatant was used. Microscopic and gel electrophoresis studies revealed yeast cell wall degradation and secretion of cellular material into the surrounding medium. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) supported the existence of enzymes in WBFB involved in bioethanol production at elevated temperatures. The results of this study will provide insight for the development of new strategies for biofuel production. |
| |
Keywords: | Bio-ethanol One-pot consecutive batch strategy Simultaneous saccharification and fermentation Cellular matrix Glycolytic and fermentation enzymes Waste from beer fermentation broth |
本文献已被 ScienceDirect 等数据库收录! |