| 同柱串联凝胶过凝从重组人肿瘤坏死因子中脱除PEG和盐 |
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| 引用本文： | 谷振宇,冯晓黎,等.同柱串联凝胶过凝从重组人肿瘤坏死因子中脱除PEG和盐[J].生物工程学报,2001,17(4):471-473. |
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| 作者姓名： | 谷振宇 冯晓黎 |
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| 作者单位： | 谷振宇（中国科学院化冶所生化工程国家重点实验室,北京,100080）
冯晓黎（中国科学院化冶所生化工程国家重点实验室,北京,100080）
苏志国（中国科学院化冶所生化工程国家重点实验室,北京,100080） |
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| 基金项目： | 国家杰出青年科学基金资助(29736180). |
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| 摘 要： |
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| 关 键 词： | 肿瘤坏死因子 破碎 双水相萃取 联合操作 串联凝胶过滤 PEG 脱除 脱盐 分离 rhTNF－α |
| 文章编号： | 1000-3061(2001)04-0471-03 |
| 修稿时间： | 2000年12月26 |
Removal of PEG and salt from recombinant human tumor necrosis factor-alpha(rhTNF-alpha) with serial operation of gel filtration chromatography] |
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| Z Y Gu,X L Feng,Z G Su.Removal of PEG and salt from recombinant human tumor necrosis factor-alpha(rhTNF-alpha) with serial operation of gel filtration chromatography][J].Chinese Journal of Biotechnology,2001,17(4):471-473. |
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| Authors: | Z Y Gu X L Feng Z G Su |
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| Institution: | State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, CAS 100080, China. |
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| Abstract: | A serial gel filtration operation with the combination of G25 and G100 in one column was developed to remove salt and PEG from upper phase rich in recombinant human tumor necrosis factor alpha after simultaneous cell disruption and two-phase aqueous extraction. Buffer exchanging and primary purification were also achieved at same time. The purification factor of 4.4 was obtained with an activity recovery of 97% by one single step of serial operation. |
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