Adsorption equilibrium and dynamics of lactase/CM-Sephadex system |
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Authors: | Şebnem Harsa Yekta Göksungur Ulgar Güvenç |
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Affiliation: | (1) Izmir Institute of Technology, Anafartalar cad. No:904, 35220 Basmane, Izmir, Turkey;(2) Dept. of Food Eng., Faculty of Engineering, Ege University, 35100 Bornova, Izmir, Turkey |
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Abstract: | Summary Partitioning behaviour and adsorption isotherms of lactase/CM-Sephadex system at equilibrium were investigated together with the adsorption kinetics in this study. Maximum adsorption was obtained at the pH values between 5.5–6.0. Adsorption isotherm was a close fit to the Langmuir model.Nomenclature a specific mass transfer area - Dm molecular diffusion coefficient (m2/sec) - e1, e2 charge of the protein and the gel - k apparent mass transfer coefficient (s-1) - ka global mass transfer coefficient - f partition coefficient - Kp dissociation constant for adsorbent-adsorbate complex, (mg/mL solvent) - p equilibrium concentration of free enzyme, (mg free enzyme/mL solution) - q equilibrium concentration of adsorbed enzyme, (mg ads./mL gel) - qm maximum adsorption capacity, (mg ads./ml gel) - Re particle Reynolds number - Sh Sherwood number - Vg/V gel volume (mL)/bulk solvent volume (mL) - Z dimensionless extent of adsorption - Kp/Po , model parameter - (/) +1 , model parameter - Vg qm / V Po , model parameter |
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