Leucyl-transfer ribonucleic acid synthetase in senescing tobacco leaves |
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| Authors: | Ilana Nathan and Amos Richmond |
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| Affiliation: | Department of Biology, University of the Negev, Beer-Sheva, Israel |
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| Abstract: | 1. The activity of leucyl-tRNA synthetase obtained from tobacco leaves declined by 50% over a period of 4 days senescence induced by detachment. In addition tRNA(Leu) from senescing leaves was charged to a lesser extent than tRNA(Leu) extracted from mature leaves immediately after detachment. 2. tRNA(Leu) was charged with a synthetase preparation from either mature or senescent leaves and chromatographed on an RPC 3 column. The elution profile showed that the marked decline in specific activity of leucyl-tRNA synthetase in senescent leaves was not associated with a loss of acylation of any isoacceptor of tRNA(Leu). |
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