A spectrophotometric determination of cyanate using reaction with 2-aminobenzoic acid |
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| Authors: | M Guilloton F Karst |
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| Affiliation: | 1. Bozok University, Medical Faculty, Department of Obstetric and Gynecology, Adnan Menderes Bulvarı No:190, Yozgat, Turkey;2. Bozok University, Medical Faculty, Department of Surgery, Yozgat, Turkey;3. Taksim Training and Education Hospital, Department of Pathology, Istanbul, Turkey;4. Siirt Kurtalan State Hospital, Department of Surgery, Siirt, Turkey;5. Ondokuz Mayıs University, Medical Faculty, Department of Surgery, Samsun, Turkey;1. IACYS-Unidad de Química Verde y Desarrollo Sostenible, Departamento de Química Orgánica e Inorgánica, Facultad de Ciencias, Universidad de Extremadura, 06006 Badajoz, Spain;2. BioLab Instituto Universitario de Bio-Orgánica Antonio González (IUBO-AG), Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain;1. Department of Biomolecular Sciences, University of Urbino Carlo Bo, Italy;2. Department of Earth, Life Sciences & Environment, University of Urbino Carlo Bo, Italy;3. Department of Base Sciences and Foundations, Chemistry Section, University of Urbino Carlo Bo, Italy;4. Department of Science and Engineering of Matter, of Environment and Urban Planning, Polytechnic University of Marche, Ancona, Italy |
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| Abstract: | A specific method has been devised for the assay of cyanate, based on the reaction with 2-aminobenzoic acid. Cyclization of the product in 6 N HCl results in the formation of 2,4(1H,3H)-quinazolinedione. Cyanate content of the samples can be measured by their absorbances at 310 nm. Alternatively, the second derivatives of the spectra can be recorded; the peak-to-peak height between the first maximum (330 nm) and the first minimum (317 nm) was shown to be proportional to the cyanate content. This method is suitable for the estimation of cyanate in aqueous solutions in the concentration range 0.01 to 2 mM. When added to blood plasma, cyanate could be detected down to 0.1 mM. |
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