Decolorization of melanin by lignin peroxidase from<Emphasis Type="Italic">Phanerochaete chrysosporium</Emphasis> |
| |
Authors: | Sung?Hwan?Woo Jeung?Suk?Cho Baek?Seok?Lee Email author" target="_blank">Eun?Ki?KimEmail author |
| |
Institution: | (1) Department of Biological Engineering, Inha University, 402-751 Incheon, Korea |
| |
Abstract: | Melanin was decolorized by lignin peroxidase fromPhanerochaete chrysosporium. This decolorization reaction showed a Michaelis-Mentens type relationship between the decolorization rate and concentration
of two substrates: melanin and hydrogen peroxide. Kinetic constants of the decolorization reaction were 0.1 OD475/min (V
max) and 99.7 mg/L (K
m) for melanin and 0.08 OD475/min (V
max) and 504.9 μM (K
m) for hydrogen peroxide, respectively. Depletion of hydrogen peroxide interrupted the decolorization reaction, indicating
the essential requirement of hydrogen peroxide. Pulsewise feeding of hydrogen peroxide continued the decolorizing reaction
catalyzed by lignin peroxidase. These results indicate that enzymatic decolorization of melanin has applications in the development
of new cosmetic whitening agents. |
| |
Keywords: | Phanerochaete chrysosporium melanin decolorization lignin peroxidase hydrogen peroxide |
本文献已被 SpringerLink 等数据库收录! |