Isolation of organelles from carrot cell suspension cultures |
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Authors: | H -D Gregor |
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Institution: | (1) Present address: Institut für Pflanzenphysiologie und Zellbiologie, Freie Universität Berlin, Berlin |
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Abstract: | Summary Organelles isolated from carrot cell suspension cultures by density gradient centrifugation and identified by their specific marker enzymes were found at the following mean densities on the sucrose gradient: microbodies 1.25 g/cm3 (catalase), mitochondria 1.18 (fumarase), endoplasmic reticulum 1.09 g/cm3 (NADH-cytochrome c reductase). Further enzyme assays were done for characterization of microbodies from carrot cultures.This work was supported by the Deutsche Forschungsgemeinschaft. |
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