Isolation of secondary metabolites of Aspergillus ochraceus by HPLC |
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Authors: | Ivonne Delgadillo |
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Affiliation: | 1. Departamento de Quimica, Universidade de Aveiro, 3800, Aveiro, Portugal
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Abstract: | A method is described for the isolation and purification of ochratoxin A, ochratoxin B, ochratoxin ß mellein, 4-hydroxymellein and penicillic acid produced byAspergillus ochraceus in a synthetic liquid medium. Ochratoxin α, which was not found in the culture medium, was obtained by acid hydrolysis of ochratoxin A. A high pressure liquid Chromatograph equipped with Lichrosorb 100 and Lichrosorb RP-18 columns and UV and/or Refractive Index detectors was used. |
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