The interaction of albumin and concanavalin A with normal and sickle human erythrocytes. |
| |
| Authors: | S J Rehfeld |
| |
| Institution: | Department of Clinical Pathology and Laboratory Medicine, University of California, San Francisco, California 94143 USA;Contribution No. 71, Center for Thermochemical Studies, Brigham Young University, Provo, Utah 84602, USA |
| |
| Abstract: | The interaction of human albumin and concanavalin A with normal and sickle human red blood cells previously washed in phosphate buffer at pH = 7.4 was studied by titration calorimetry. The amount of albumin bound to normal cells was (6.8 ± 2.2) × 105 molecules/cell. An equilibrium constant of 5 × 1010 and an enthalpy change of ?(280 ± 90) kcal/mol albumin was determined for albumin interaction with normal cells. The amount of albumin bound to sickle cells was (12.4 ± 1.0) × 105 molecules/cell and the enthalpy change for albumin interaction with sickle cells was ?(390 ± 140) kcal/mol. Normal cells bound (5.7 ± 2.4) × 105 concanavalin A molecules/cell with an enthalpy change of ?(840 ± 200) kcal/mol concanavalin. All experiments were conducted at 25°C. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
