Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly |
| |
Authors: | Carazo-Salas R E Gruss O J Mattaj I W Karsenti E |
| |
Institution: | European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. carazosa@embl-heidelberg.de |
| |
Abstract: | It was recently reported that GTP-bound Ran induces microtubule and pseudo-spindle assembly in mitotic egg extracts in the absence of chromosomes and centrosomes, and that chromosomes induce the assembly of spindle microtubules in these extracts through generation of Ran-GTP. Here we examine the effects of Ran-GTP on microtubule nucleation and dynamics and show that Ran-GTP has independent effects on both the nucleation activity of centrosomes and the stability of centrosomal microtubules. We also show that inhibition of Ran-GTP production, even in the presence of duplicated centrosomes and kinetochores, prevents assembly of a bipolar spindle in M-phase extracts. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|