Histidine:pyruvate transamination and phyenylalanine:pyruvate transamination may be catalyzed by the same enzyme. |
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Authors: | J C Shih R H Chiu Y L Chan |
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Affiliation: | School of Pharmacy University of Southern California Los Angeles, California 90033 USA |
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Abstract: | Some properties of histidine:pyruvate transaminase (HPT) and phenylalanine:pyruvate transaminase (PPT) in the cytosol of rat liver were studied. HPT and PPT activity could not be separated by DEAE-Sephadex A-50 or hydroxylapatite column chromatography, and the ratio of activity remained constant during these purification procedures. The two enzyme activities also showed similar heat stability and responses to glucagon injection. Based on these findings, we suggest that a single enzyme may specifically catalyze histidine:pyruvate and phenylalanine:pyruvate transamination. |
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