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Isolation and characterization of fourteen microsatellite loci for striped field mouse (Apodemus agrarius)
Authors:Hua Wu  Xiang-Jiang Zhan  Li Yan  Shao-Ying Liu  Ming Li  Jin-Chu Hu  Fu-Wen Wei
Affiliation:(1) Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, People’s Republic of China;(2) Sichuan Academy of Forestry, Chengdu, 610066, Sichuan, People’s Republic of China;(3) Institute of Rare Animals and Plants, China West Normal University, Nanchong, 637002, Sichuan, People’s Republic of China
Abstract:We describe the isolation and characterization of 14 microsatellite loci in the striped field mouse from genomic DNA-enriched libraries in this paper. The 14 new loci were tested in 24 individuals from four populations in Southwest China. These loci were highly polymorphic with numbers of alleles per locus ranging from 6 to 14 and expected heterozygosities from 0.789 to 0.925. All loci followed Hardy–Weinberg expectations except SFM4, SFM11 and SFM13 loci. No significant linkage association was found among all loci. The 14 novel polymorphic microsatellite loci will be useful in studying phylogeography and population genetics of the striped field mouse.
Keywords:Striped field mouse (Apodemus agrarius)  Microsatellites  Heterozygosity  Phylogeography  Population genetics
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