Mitochondrial carbonic anhydrase in osteoclasts and two different epithelial acid-secreting cells |
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Authors: | Eeva-Kaisa Karhukorpi Päivi Lakkakorpi Nicholas Carter Susan Dodgson and Kalervo Väänänen |
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Institution: | (1) Department of Anatomy, University of Oulu, Kajaanintie 52A, SF-90220 Oulu, Finland;(2) Department of Child Health, St. Georges Hospital, Medical School, University of London, SW 17 ORE, UK;(3) Department of Physiology, University of Pennsylvania, Philadelphia, USA |
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Abstract: | Summary Acid secreting cells are rich in mitochondria and contain high levels of cytoplasmic carbonic anhydrase II. We have studied the ultrastructural distribution of a mitochondrial isoenzyme, carbonic anhydrase V, in two different acid-secreting epithelial cells, gastric parietal cells and kidney intercalated cells as well as in osteoclasts, which are the main bone resorbing cells. The mitochondria differ in carbonic anhydrase V content in these three acid-producing cells: gastric parietal cell mitochondria show strong immunolabelling for this isoenzyme, osteoclast mitochondria faint labelling and kidney intercalated cell mitochondria no labelling. The immunolabelling was located in the mitochondrial matrix, often in close contact with the inner mitochondrial membrane. These results show that mitochondrial carbonic anhydrase levels are not related to acid-transporting activity. |
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