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An automatic enzymatic determination of glycogen in a continuous-flow system without interference of free glucose. |
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| Authors: | B Eissle G Heinzel |
| Institution: | Department of Biochemistry Dr. K. Thomae GmbH Biberach/Riß Federal Republic of Germany |
| Abstract: | An autoanalyzer system for enzymatic determination of glycogen is described. Free glucose is eliminated by hexokinase/glucose-6-phosphate dehydrogenase. Glycogen is hydrolyzed by amylo-1,6-glycosidase at pH 4,8, and the resulting glucose is dialyzed and determined by the hexokinase/glucose-6-phosphate dehydrogenase reaction. |
| Keywords: | Address reprint requests to this author Public Health Research Institute 455 First Avenue New York N Y 10016 |
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