Identification of macrophage-related candidate genes in lupus nephritis using bioinformatics analysis |
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| Affiliation: | 1. Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky;2. Robley Rex Veterans Affairs Medical Center, Louisville, Kentucky;1. Organ Transplantation Center, Sichuan Academy of Medical Sciences and Sichuan Provincial People''s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, Sichuan province, China;2. Organ Transplantation translational medicine Key laboratory of Sichuan province,Chengdu, Sichuan 610072, China;3. Women and Children Health Care Center of Luoyang, Luoyang 471000, Henan province, China;4. People''s Hospital of Changshou Chongqing, Chongqing 401220, China;5. North Sichuan Medical College, Nanchong 637100, Sichuan province, China;6. Human Islet Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston 02114, MA, USA |
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| Abstract: | Lupus nephritis (LN) is a chronic autoimmune disorder. Here we try to identify the candidate genes in macrophages related to LN. We performed a systematic search in the Gene Expression Omnibus (GEO) database for microarray in human mononuclear cells and mouse macrophages of LN. The results of clustering and venn analysis of different GEO datasets showed that 8 genes were up-regulated and 2 genes down-regulated in samples from both human and mouse LN. The data from gene network and GO analysis revealed that CD38 and CCL2 were localized in the core of the network. Immunofluorescence staining showed that CD38 expression was markedly increased in macrophages from kidneys with LN. Our study identifies the gene expression profile for macrophages and demonstrated the induction of CCL2 and CD38 in macrophages from patients with LN. However, regarding the limited patient number included in this study, the results are preliminary and more studies are still needed to further decipher the macrophage-related candidate genes for the pathogenesis of LN. |
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