| 影响苏云金芽孢杆菌基因在转基因植物中表达的因素 |
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| 引用本文： | 卢美贞,崔海瑞,姚艳玲,忻雅.影响苏云金芽孢杆菌基因在转基因植物中表达的因素[J].细胞生物学杂志,2005,27(5):509-513. |
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| 作者姓名： | 卢美贞 崔海瑞 姚艳玲 忻雅 |
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| 作者单位： | 浙江大学原子核农业科学研究所,杭州310029 |
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| 基金项目： | 国家自然科学基金资助项目（No.30270273） |
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| 摘 要： | 苏云金芽孢杆菌（Bacillus thuringiensis，Bt）杀虫晶体蛋白基因是植物抗虫基因工程中应用最广泛的基因资源。影响Bt基因在转基因植物中表达的因素繁多，阐明这些因素的效应对于获得Bt基因在受体植物中的稳定高效表达具有重要意义。现对Bt基因表达的主要影响因子，如Bt基因表达单元、植物发育、外部环境条件、受体植物遗传背景、整合位点及Bt基因沉默现象等进行了综述。
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| 关 键 词： | 苏云金芽孢杆菌 转基因植物 基因表达 抗虫基因 整合位点 |
| 收稿时间： | 2004-11-03 |
| 修稿时间： | 2005-05-17 |
Factors Affecting Expression of Bacillus thuringiensis Genes in Transgenic Plants |
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| Mei-Zhen Lu, Hai-Rui Cui, Yan-Ling Yao, Ya Xin.Factors Affecting Expression of Bacillus thuringiensis Genes in Transgenic Plants[J].Chinese Journal of Cell Biology,2005,27(5):509-513. |
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| Authors: | Mei-Zhen Lu Hai-Rui Cui Yan-Ling Yao Ya Xin |
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| Institution: | Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | The insecticidal crystal protein gene from Bacillus thuringiensis (Bt) is the most widely used resource in plant engineering for insect resistance to date. There are many factors affecting Bt gene expression, elucidating their effects is important to acquire stable and high expression of Bt gene in transgenic plants. Effects of main factors on Bt gene expression, such as the expression cassette of Bt gene, plant development, external environ- mental conditions, genetic background of receptors and integration site in plant genome, and Bt gene silence were reviewed in the paper. |
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| Keywords: | transgenic plants Bt gene expression affecting factors |
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