矮泰引-3中半矮秆基因的分子定位

矮泰引-3的矮生性状受两对独立遗传的半矮秆基因控制，利用SSR标记将这两个矮秆基因分别定位到第1和第4染色体上。等位性测交的结果表明，位于第1染色体上的矮秆基因与sd1是等位的，所以仍然称其为sd1；而位于第4染色体上的矮秆基因是一个新基因，暂命名为sdt2。利用SSR标记将sd1定位于RM297、RM302和RM212的同一侧，而与OSR3共分离，它们之间的位置关系可能是RM297-RM302-RM212-OSR3-sd1，遗传距离分别为4．7cM、0cM、0．8cM和0cM，这与sd1在第1染色体长臂上的确切位置是基本一致的。利用已有的SSR标记和拓展的SSR标记将sdt2定位于SSR332、RM1305和RM5633、RM307、RM401之间，它们的排列位置可能是SSR332-RM1305-sdt2-RM5633-RM307-RM401，它们之间的遗传距离分别为11．6cM、3．8cM、0．4cM、0cM和0．4cM。

Molecular Mapping of Two Semidwarf Genes in an indica Rice Variety Aitaiyin3 (Oryza sativa L.)

The rice dwarf variety Aitaiyin3 is derived from a semidwarf cultivar Taiyin1.Genetic analysis indicated that the dwarf phenotype in Aitaiyin3 is involved in two recessive loci.Using bulk analysis with SSR markers,the two loci were located on the chromosome 1 and chromosome 4,respectively.Moreover,the allelism test showed that the locus on the chromosome 1 is allelic to the semidwarf gene sd1,while the other one is a new locus,here was named as sdt2.Further molecular mapping showed that sd1 was linked with SSR markers in the order of RM297-RM302-RM212-OSR3-sd1 with genetic distances of 4.7 cM,0 cM,0.8 cM and 0 cM,respectively.The linkage relationship of sdt2 with five SSR markers was SSR332-RM1305-sdt2-RM5633-RM307-RM401 with genetic distances of 11.6 cM,3.8 cM,0.4 cM,0 cM and 0.4 cM,respectively.